Polymorphism of TLR1, TLR4, and SLC11A1 genes in populations of different cattle breeds of Ukrainian selection

Kulibaba, R. O.; Liashenko, Yuriy; Иващенко, О. А. Берилло В. А. Хайленко А. Т.

doi:10.15407/agrisp8.03.025

Cited by 3 publications

(7 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The number of animals, homozygous for allele G, as compared to homozygotes AA was high 90 against 0. By this index, meat Charolais cattle was similar to the Ukrainian Gray cattle of dual-purpose cows, which did not have animals with genotype AA either, although AG and GG percentages were substantially different (Kulibaba et al, 2021). For TLR4, our fi ndings were completely opposite to those of TLR1.…”

Section: Discussionmentioning

confidence: 55%

“…This study is a continuation of our previous investigations which focused on polymorphism of genes TLR1, TLR4, and SLC11A1 in populations of Ukrainian Black-and-White dairy breed, Ukrainian Red-and-White dairy breed, Ukrainian Gray cattle (Kulibaba et al, 2021;Ivashchenko & Kulibaba, 2022). In this case, our focus is on studying beef cattle to address the question: Do the observed patterns of allele and genotype frequencies at identifi ed polymorphic loci align with general trends, or do they characterize cattle breeds based on their productivity direction (meat or dairy)?…”

Section: Introductionmentioning

confidence: 64%

“…The most prevalent complex genotype was 7400 СС /7808 АА , up to 75 % of the total number of animals in the population. As it was discovered in our previous studies (Kulibaba et al, 2021), incomplete activity of PstI may lead to possible mistakes in the genotyping of animals indicating the need for strict compliance with the restriction procedure (primarily due to the large number of fragments).…”

Section: Resultsmentioning

confidence: 93%

“…This locus showed the highest value of the effective number of alleles (1.98) among the 5 genes studied, which actually is the maximal level of polymorphism for two-allele systems. Negative values of the fi xation index were also noted for other cattle breeds reared in Ukraine (Kulibaba et al, 2021), but they did not reach such extreme values. The number of animals, homozygous for allele G, as compared to homozygotes AA was high 90 against 0.…”

Section: Discussionmentioning

confidence: 90%

“…The results obtained are in full agreement with other data obtained for dairy and dual purpose cattle. The locus was monomorphic also in cattle breeds of dairy (Ukrainian Black-and-White dairy breed, Ukrainian Red-and-White dairy breed) and combined (Ukrainian Gray cattle) directions of productivity (Kulibaba et al, 2021;Okuni et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

Gene polymorphism in a population of Charolais beef cattle using DNA-markers

Kulibaba,

Liashenko,

Kulibaba

et al. 2024

Agric. sci. pract.

View full text Add to dashboard Cite

Aim. To study the polymorphism of genes, associated with resistance to different diseases (TLR1, TLR4, SLC11A1, TLR1, TLR4, IFNGR2, SLC11A1, TNFα, and MBL1) in the population of Charolais beef cattle. Methods. The studies were conducted using the methods of polymerase chain reaction and the restriction fragment length polymorphism (PCR-RFLP); the artificially created restriction site-PCR (ACRS-PCR) was used to study the polymorphism of gene MBL1. Results. In the population (n = 100) of Charolais cattle, genes TLR1, IFNGR2, SLC11A1, TNFα, and MBL1 were polymorphic, while TLR4 was found to be monomorphic by three mutant variants (8732G>A, 8834G>C, and 2021C>T). BclI-polymorphism of the first exon was used to determine the excess of heterozygous animals (81.8 %); there were no animals homozygous by allele A. By polymorphism of locus SLC11A1 for SNP6 (7808A>T), we deter- mined complete domination of the frequency of allele A over T (0.985 vs 0.015), no animals, homozygous by allele TT, were found. As for SNP5 7400C>G, there were no animals homozygous for allele G; the ratio of alleles C and G was 0.79 and 0.21, respectively. By locus IFNGR2 (1008A>G), we determined the prevalence of the frequency of al- lele A over G (0.745 vs 0.255); the animals with genotype AA were the most common. As for gene TNFα (–824A>G), we determined a two-fold prevalence of the frequency of allele G over A due to a higher number of animals with geno- type GG (39 %). Some 50 % of the animals were heterozygous for this gene.By locus MBL1,we found the prevalence of the frequency of allele G (StyI-) as compared to A (StyI+) due to a considerable number of homozygous animals GG (64 %). The percentage of homozygous animals was 37.8 %. Conclusions. General characteristics of genetic variability of the Charolais beef cattle population were determined for the loci TLR1, TLR4, IFNGR2, SLC11A1, TNFα, and MBL1. As for SNP6 7808A>T of locus SLC11A1, we have determined the final stage of allele A fixation, by the results of which the gene will become completely monomorphic by this mutation. The results of the analysis of the ratio between the frequencies of alleles and genotypes by the revealed polymorphic markers demonstrated that it is unreasonable to use animals with different allelic variants of genes TLR1, IFNGR2, SLC11A1 in further studies on the search for associations with the manifestation of economically valuable traits in Charolais beef cows of the experimental population due to insufficient number of animals with different genotypes. For loci TNFα and MBL1, we found enough animals with different genotypes to conduct further association studies. The deviation from the genetic Hardy-Weinberg equilibrium was observed for the loci TLR1, SLC11A1 (7400C>G), and MBL1 in the population.

show abstract

Section: Discussionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 64%

Section: Resultsmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Gene polymorphism in a population of Charolais beef cattle using DNA-markers

Kulibaba,

Liashenko,

Kulibaba

et al. 2024

Agric. sci. pract.

View full text Add to dashboard Cite

show abstract

Characterization and Computational Investigation of Polymorphisms in the TIR Domain of the TLR4 Protein in Holsteins Raised in Turkey

Atalay

2023

Journal of Research in Veterinary Medicine

View full text Add to dashboard Cite

Increasing environmental temperatures as a result of climate change adversely affect livestock welfare and health. Moreover, temperatures increase the distribution and survival of parasites and infectious agents. Livestock diseases that cause significant economic losses are a worldwide concern. Toll like receptor 4 (TLR4) is an ideal marker gene candidate, due to the key role it plays in initiating the immune response against pathogens. In this study, the toll-interleukin-1 receptor (TIR) domain polymorphisms were investigated in the Turkish Holstein cattle by DNA sequencing. The effects of polymorphisms on the protein structure and function were evaluated by computational tools (I-Mutant Suite, Project Hope and PyMOL). The rs8193069 C>T polymorphism was detected in the TIR domain. The SNP causes threonine to isoleucine substitution at position 674 of the TLR4 protein which is the second amino acid of the TIR domain. The I-Mutant Suite predicted that Thr674Ile substitution can result in decreased protein stability (DDG= -0.40 Kcal/mol). Project Hope results showed that mutant and wild-type amino acids have different properties and that this can disturb the TIR domain. The Thr674Ile polymorphism observed in the TIR domain of TLR4 protein in Turkish Holstein cattle might affect the function of the TLR4 by causing physicochemical changes.

show abstract

Productivity of Cows of the Ukrainian Red-Spotted Dairy Breed with Different Genotypes According to the TLR1, SLC11A1 and CSN2 Loci

Иващенко¹,

Kulibaba²

2022

UBSRAS

View full text Add to dashboard Cite

The use of methods of marker-associated selection opens up new perspectives in the context of maximum realization of the productive potential of animals, which makes the issue of the application of modern scientific approaches in animal husbandry particularly relevant. Considering this, the analysis of the productive qualities of cattle with different genotypes according to the set of candidate genes is one of the most urgent tasks of animal genetics and breeding. Therefore, the purpose of this study is to analyze the productive qualities of cows of the Ukrainian red-spotted dairy breed with different genotypes according to the TLR1, SLC11A1 and CSN2 loci. Genotyping of cattle individuals was performed using the PCR-RFLP method for TLR1 and SLC11A1 and AS-PCR for CSN2. The analysis of productive qualities of animals was carried out by comparing the parameters of three lactations for each group of animals according to the indicators of average milk yield for 305 days of lactation (kg), protein content in milk (%) and milk fat content (%). The study was carried out on the basis of previously obtained results of individual typing of individuals according to research loci. According to the results of studies conducted in the experimental population of cows for the toll-like receptor 1 (TLR1) locus for the first and third lactations, the dominant values of the yield index are characteristic of homozygotes with the GG genotype, while for the second lactation the highest values of yield are observed for homozygous individuals for allele A. At the same time, a probable difference between the indicators of individuals with different genotypes was not established.No significant difference between the groups of cows with the studied genotypes in the parameters of fat and protein content in milk by the TLR1 locus was also found. SLC11A1 is characterized by a characteristic difference from the previous gene, as two mutations forming haplotypes – CC-AA, СG-AA and CG-AT out of nine potentially possible variants were found in this locus. According to indicators of milk productivity, animals with the СG-AA haplotype had higher values of milk compared to other haplotypes during all three lactations (the difference is probable). The greatest differences were observed with the CG-AT haplotype. According to the indicators of the content of milk protein and fat, no probable differences between individuals with different haplotypes were established. The variability of signs for all lactations was at a low level. As for the beta-casein locus, according to the results of the conducted research, no significant difference in the indicators of milk productivity of cows with different genotypes was found. The highest confidence values are characteristic of individuals with the A1A1 genotype, however, the difference with individuals with other genotypes is unlikely. In any case, the A2 allele, according to the value of the indicators of homozygous individuals (genotype A2A2), does not have a negative effect on the parameters of milk productivity of animals

show abstract

Polymorphism of TLR1, TLR4, and SLC11A1 genes in populations of different cattle breeds of Ukrainian selection

Cited by 3 publications

References 29 publications

Gene polymorphism in a population of Charolais beef cattle using DNA-markers

Gene polymorphism in a population of Charolais beef cattle using DNA-markers

Characterization and Computational Investigation of Polymorphisms in the TIR Domain of the TLR4 Protein in Holsteins Raised in Turkey

Productivity of Cows of the Ukrainian Red-Spotted Dairy Breed with Different Genotypes According to the TLR1, SLC11A1 and CSN2 Loci

Contact Info

Product

Resources

About