Polymorphism rs11200638 enhanced HtrA1 responsiveness and expression are associated with age-related macular degeneration

He, Fenglan; Li, Xiaohong; Cai, Suping; Lu, Lihua; Zhang, Tong; Yang, Ming; Fan, Ning; Wang, Xizhen; Liu, Xuyang

doi:10.1038/s41433-021-01706-8

Cited by 7 publications

(5 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ARPE-19 cells were incubated with different concentration of LPS (0, 0.1 µg/mL, 0.5 µg/mL, 1 µg/mL), and the HTRA1 protein secreted into the medium was detected by western blot. Consistent with the results of other studies [4,11], the level of HTRA1 protein increased with the addition of LPS. The induction effect on HTRA1 expression of LPS increased with the concentration of LPS and saturated at 1 µg/mL.…”

Section: Lps Induces Htra1 Expression In Arpe-19 Cellssupporting

confidence: 92%

“…The effect of HTRA1 on the growth is controversial. Several studies reported HTRA1 increases proliferation of ARPE-19 cells and regulated cell migration [5,8], but some research found that HTRA1 overexpression resulted in significant apoptotic changes and inhibited cell growth [4]. To determine the effect of HTRA1 on the proliferation of ARPE-19 cells, Edu was used to detect cell proliferative capacity.…”

Section: Elevated Htra1 Level Inhibits Proliferation Of Arpe-19 Cellsmentioning

confidence: 99%

“…Through genome-wide association analysis it is found that complement factor H (CFH) and HTRA1 gene serves as two major genes related to the occurrence and development of AMD. More than half of AMD patients have singlenucleotide polymorphisms of these two genes [3,4]. At the same time, a considerable number of studies have proposed that overexpression of HTRA1 protein in retinal pigment epithelial (RPE) cells can induce the occurrence of AMD [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lipopolysaccharide Activated NF-kB Signaling by Regulating HTRA1 Expression in Human Retinal Pigment Epithelial Cells

Pan

Liu

et al. 2023

Molecules

View full text Add to dashboard Cite

Inflammation and elevated expression of high temperature requirement A serine peptidase 1 (HTRA1) are known high risk factors for age-related macular degeneration (AMD). However, the specific mechanism that HTRA1 causes AMD and the relationship between HTRA1 and inflammation remains unclear. We found that lipopolysaccharide (LPS) induced inflammation enhanced the expression of HTRA1, NF-κB, and p-p65 in ARPE-19 cells. Overexpression of HTRA1 up-regulated NF-κB expression, and on the other hand knockdown of HTRA1 down-regulated the expression of NF-κB. Moreover, NF-κB siRNA has no significant effect on the expression of HTRA1, suggesting HTRA1 works upstream of NF-κB. These results demonstrated that HTRA1 plays a pivotal role in inflammation, explaining possible mechanism of overexpressed HTRA1-induced AMD. Celastrol, a very common anti-inflammatory and antioxidant drug, was found to suppress inflammation by inhibiting phosphorylation of p65 protein efficaciously in RPE cells, which may be applied to the therapy of age-related macular degeneration.

show abstract

Section: Lps Induces Htra1 Expression In Arpe-19 Cellssupporting

confidence: 92%

Section: Elevated Htra1 Level Inhibits Proliferation Of Arpe-19 Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lipopolysaccharide Activated NF-kB Signaling by Regulating HTRA1 Expression in Human Retinal Pigment Epithelial Cells

Pan

Liu

et al. 2023

Molecules

View full text Add to dashboard Cite

show abstract

Section: The Influence Of Genetic Factors On the Development Of Amdmentioning

confidence: 99%

“…Studies confirmed that single nucleotide polymorphisms (SNPs) in the promoter region of HtrA1 strongly correlates with the development of AMD [ 189 , 191 , 235 ]. This polymorphism increased the expression of HtrA1, but further influence on the development of AMD is unclear [ 189 ]. Studies conducted in Chinese population showed, that polymorphisms in these genes ( rs11200638 and rs3793917 ) play role in progression of AMD [ 209 ].…”

Section: The Influence Of Genetic Factors On the Development Of Amdmentioning

confidence: 99%

Immunogenetic and Environmental Factors in Age-Related Macular Disease

Brodzka,

Baszyński,

Rektor

et al. 2024

IJMS

View full text Add to dashboard Cite

Age-related macular degeneration (AMD) is a chronic disease, which often develops in older people, but this is not the rule. AMD pathogenesis changes include the anatomical and functional complex. As a result of damage, it occurs, in the retina and macula, among other areas. These changes may lead to partial or total loss of vision. This disease can occur in two clinical forms, i.e., dry (progression is slowly and gradually) and exudative (wet, progression is acute and severe), which usually started as dry form. A coexistence of both forms is possible. AMD etiology is not fully understood. Extensive genetic studies have shown that this disease is multifactorial and that genetic determinants, along with environmental and metabolic-functional factors, are important risk factors. This article reviews the impact of heavy metals, macro- and microelements, and genetic factors on the development of AMD. We present the current state of knowledge about the influence of environmental factors and genetic determinants on the progression of AMD in the confrontation with our own research conducted on the Polish population from Kuyavian-Pomeranian and Lubusz Regions. Our research is concentrated on showing how polluted environments of large agglomerations affects the development of AMD. In addition to confirming heavy metal accumulation, the growth of risk of acute phase factors and polymorphism in the genetic material in AMD development, it will also help in the detection of new markers of this disease. This will lead to a better understanding of the etiology of AMD and will help to establish prevention and early treatment.

show abstract

Relationship between CCL2 gene 2518A/G (rs1024611) polymorphism and age-related macular degeneration susceptibility: meta-analysis and trial sequential analysis

Tian,

Xu,

Wen

et al. 2024

Int Ophthalmol

View full text Add to dashboard Cite

Polymorphism rs11200638 enhanced HtrA1 responsiveness and expression are associated with age-related macular degeneration

Cited by 7 publications

References 37 publications

Lipopolysaccharide Activated NF-kB Signaling by Regulating HTRA1 Expression in Human Retinal Pigment Epithelial Cells

Lipopolysaccharide Activated NF-kB Signaling by Regulating HTRA1 Expression in Human Retinal Pigment Epithelial Cells

Immunogenetic and Environmental Factors in Age-Related Macular Disease

Relationship between CCL2 gene 2518A/G (rs1024611) polymorphism and age-related macular degeneration susceptibility: meta-analysis and trial sequential analysis

Contact Info

Product

Resources

About