Polymorphism within TATA-box of bovine lactoferrin gene and its association with performance traits in Holstein cattle

Zabolewicz, Tadeusz; Brym, Paweł; Oleński, Kamil; Suchocki, Tomasz; Malewski, Tadeusz; Szyda, Joanna; Kamiński, Stanisław

doi:10.1016/j.livsci.2012.07.020

Cited by 4 publications

(9 citation statements)

References 35 publications

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“…Since lactoferrin is involved in response to bacterial infection (Chaneton et al 2008), it is more interesting to show the overall effect of LTF polymorphism faced by health status of mammary gland than to trace its changes in the following stages of lactation. As we proved earlier, using the EMSA technique (Zabolewicz et al 2012), a promoter sequence with the A allele was more efficiently bound by the nuclear proteins isolated from healthy mammary tissues than a promoter containing the C allele. This observation is in accordance with results published by Bahar et al (2011) who demonstrated that promoter constructs, including allele A, exhibited 1.44 -fold higher basal transcriptional activity.…”

Section: Discussionsupporting

confidence: 60%

“…From 79 SNPs (Single Nucleotide Polymorphisms) identified in the bovine LTF gene , Li et al 2004, Daly et al 2006, O'Halloran et al 2009, Huang et al 2010, Bahar et al 2011, substitution of A/C in position -28 within the TATA -box seems to have the most probable influence on LTF gene expression in the mammary gland. As reported by O'Halloran et al (2010), Bahar et al (2011) and our group (Zabolewicz et al 2012) this SNP located in the key regulatory motif responsible for initiation of the gene transcription, affects the transcription complex and is associated with routine traits recorded in Holstein cattle such as SCC. So far, however, little is known about the effect of A/C point mutation within the TATA box on the LTF concentration, considering the health status of the udder.…”

mentioning

confidence: 53%

“…The results of many studies confirmed that LTF gene may be considered as QTL for milk performance traits and natural resistance to mastitis (Seyfert et al 1996, Li et al 2004, Daly et al 2006, Kamiński et al 2006, Huang et al 2010, Sender et al 2010, Bahar et al 2011, Zabolewicz et al 2012, Wojdak-Maksymiec et al 2013). …”

Section: Discussionmentioning

confidence: 85%

“…The -28 A/C polymorphism within the TATA-box of the LTF promoter was identified by the PCR-SSCP method published by our group earlier (Zabolewicz et al 2012). …”

Section: Snp Detectionmentioning

confidence: 99%

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Association of polymorphism within LTF gene promoter with lactoferrin concentration in milk of Holstein cows

Zabolewicz¹,

Barcewicz²,

Brym³

et al. 2014

Polish Journal of Veterinary Sciences

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This study analyzed the association between single nucleotide polymorphism (A/C) in position -28 located in the TATA box of LTF gene and the lactoferrin concentration in bovine milk secreted by healthy and infected udders. Out of 241, 69 cows were selected into the experimental group and were divided into 3 groups according to mean value of somatic cell count (SCC): I <180 000 cells/mL, II: 180 000 -350 000 cells/mL and III > 350 000 cells/mL. In each SCC group, three LTF genotypes: AA, AC and CC were identified by PCR-SSCP method. A total of 604 milk samples were collected monthly and lactoferrin concentration was measured by ELISA. The 1-way ANOVA within SCC groups was performed to estimate association of -28 A/C genotypes with mean lactoferrin concentration per lactation. In the group of healthy cows (<180 000 cells/mL) LTF concentration in milk cows with the AA genotype (107.58 ± 17.92 μg/mL) was significantly higher than in homozygotes CC (52.09 ± 19.01 μg/mL). Unexpectedly, in cows with elevated SCC (>350 000 cells/mL) we observed a significant opposite relationship (207.21 ± 28.50 in CC vs 115.0 ± 28.6 μg/mL in AA). We hypothesized that a promoter with allele C, which cannot be recognized as a TATA sequence is becoming more accessible for other transcription factors, which may induce alternative LTF gene expression. We assume that our results demonstrate a very interesting effect of differential gene expression depending on polymorphism in a key regulatory motif (TATA box) and also on the health status of mammary tissues.

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Section: Discussionsupporting

confidence: 60%

mentioning

confidence: 53%

Section: Discussionmentioning

confidence: 85%

“…The -28 A/C polymorphism within the TATA-box of the LTF promoter was identified by the PCR-SSCP method published by our group earlier (Zabolewicz et al 2012). …”

Section: Snp Detectionmentioning

confidence: 99%

See 2 more Smart Citations

Association of polymorphism within LTF gene promoter with lactoferrin concentration in milk of Holstein cows

Zabolewicz¹,

Barcewicz²,

Brym³

et al. 2014

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

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“…ABCG2, DGAT1 CASB, IGF2 and GHR genes are among these exceptions, but not without inconsistency in the results (Komisarek et al, 2004, Bagnicka et al, 2010, Olsen et al, 2007, Olenski et al, 2012. It has been shown that genes, connected originally to health and development traits, like IL8, IL8R, CCL2, CCR2, can be associated with dairy performance.…”

Section: Introductionmentioning

confidence: 99%

Association between lactoferrin single nucleotide polymorphisms and milk production traits in Polish Holstein cattle

et al. 2014

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Bovine lactoferrin exhibits strong potential for further applications as a mastitis resistance marker. Since selection for mastitis resistance should not interfere with dairy performance, we investigated the association between bovine lactoferrin gene polymorphism and production traits in Polish Holsteins. The associations between four SNPs, localized in the 5'-flanking region and in exons 4 and 9 of the lactoferrin gene, and dairy performance were examined. SNPs were associated with almost all test-day milk performance traits. Significant associations were found between lactoferrin genotypes and the estimated breeding values for those traits. To find out whether the discrepancies between the lactoferrin gene SNP's influence on phenotype (test-day milk performance) and on estimated breeding values originate from the impact of other factors, we explored the genotype by environment interaction. Substantial impacts of SCC, lactation stage and parity were found. This paper suggests that the genotype by environment interaction may significantly change associations between genes and traits. It is important to include similar analyses to the studies on disease markers before using them in the selection.

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Genetic variability detected at the lactoferrin locus (LTF) in the Italian Mediterranean river buffalo

et al. 2016

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Lactoferrin (LTF) is multi-functional protein belonging to the whey protein fractions of the milk. The gene LTF encoding for such protein is considered a potential candidate for body measurement, milk composition and yield. This study reports on the genetic variability at LTF locus in the Italian Mediterranean river buffalo and its possible association with milk yield. Eleven polymorphic sites were found in the DNA fragment spanning the exons 15-16. In particular, the intron 15 was extremely polymorphic with 9 SNPs detected, whereas the remaining 2 SNPs were exonic mutations (g.88G>A at the exon 15 and g.1351G>A at the exon 16) and both synonymous. The genotyping of the informative samples evidenced 3 haplotypes, whose frequencies were 0.6; 0.3 and 0.1 respectively, whereas the analysis of the exonic SNPs showed a perfect condition of linkage disequilibrium (g.88A/g.1351G and g.88G/g.1351A). The association study carried out by using the SNP g.88G>A showed that buffalo LTF gene has no statistically significant influence on daily milk yield. This study adds knowledge to the genetic variability of a species less investigated than the other ruminant species, that may serve as a useful tool for large-scale screening of buffalo populations

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Polymorphism within TATA-box of bovine lactoferrin gene and its association with performance traits in Holstein cattle

Cited by 4 publications

References 35 publications

Association of polymorphism within LTF gene promoter with lactoferrin concentration in milk of Holstein cows

Association of polymorphism within LTF gene promoter with lactoferrin concentration in milk of Holstein cows

Association between lactoferrin single nucleotide polymorphisms and milk production traits in Polish Holstein cattle

Genetic variability detected at the lactoferrin locus (LTF) in the Italian Mediterranean river buffalo

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