Polymorphisms and Evolution of Solanum bulbocastanum Genes for Broad-Spectrum Resistance to Phytophthora infestans

Fadina, O. A.; Бекетова, М. П.; Кузнецова, М. А.; Рогозина, Е. В.; Хавкин, Э. Е.

doi:10.1134/s1021443719060062

Cited by 4 publications

(6 citation statements)

References 24 publications

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“…The marker of this gene, Rpi-blb1-821, and the marker of its orthologue, Rpi-sto1-890, which are located widely apart on the gene sequence, were tightly linked in S. bulbocastanum accessions and two Longipedicellata accessions ( Tables S2 and S9 ). The sequences of Rpi-blb1/Rpi-sto1 amplicons corresponding to these markers (the NCBI Genbank accessions KP317986–KP317990) were 100% identical to the prototype genes AY426259 and EU884421 [ 53 ]. In this study, we also discovered both markers, Rpi-blb1-821 and Rpi-sto1-890, in the S. cardiophyllum accession k-24203-435 ( Table S3 ).…”

Section: Resultsmentioning

confidence: 99%

“…In this study, we also discovered both markers, Rpi-blb1-821 and Rpi-sto1-890, in the S. cardiophyllum accession k-24203-435 ( Table S3 ). The fragments cloned from this accession were 99% identical to the prototype genes and differed by several SNPs (see also [ 53 ]). The function of these fragments has not yet been established.…”

Section: Resultsmentioning

confidence: 99%

“…The VIR accessions of Petota species have been repeatedly assessed for LB resistance, and new sources of LB resistance were identified [ 20 , 53 , 77 , 78 , 79 ]. Leaf LB resistance is characteristic of the North American species from series Demissa ( S. demissum , S. guerreroense Corr.…”

Section: General Discussion and Conclusionmentioning

confidence: 99%

“…The gene Rpi-blb1 was firmly established as the marker of genome B in the series Bulbocastana and Longipedicellata rather than Pinnatisecta [ 3 , 53 ]. There are, however, several exceptions.…”

Section: General Discussion and Conclusionmentioning

confidence: 99%

“…In addition to S. cardiophyllum , a full-length homolog of the Rpi-blb1 gene was found in the genome of S. pinnatisectum [ 75 ], its sequence (CP047564) is 84% identical to Rpi-blb1 . Fadina et al [ 53 ] did not find the SCAR markers of the Rpi-blb1/Rpi-sto1 genes in the accessions of Demissa and Pinnatisecta (with exception of a single S. cardiophyllum accession k-24207, GLKS2152, 420). The markers of Rpi-blb1/Rpi-sto1 were also absent from resistant S. verrucosum accessions comprising the RBver homolog [ 96 ].…”

Section: General Discussion and Conclusionmentioning

confidence: 99%

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Diversity of Late Blight Resistance Genes in the VIR Potato Collection

Рогозина

Gurina

Chalaya

et al. 2023

Plants

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Late blight (LB) caused by the oomycete Phytophthora infestans (Mont.) de Bary is the greatest threat to potato production worldwide. Current potato breeding for LB resistance heavily depends on the introduction of new genes for resistance to P. infestans (Rpi genes). Such genes have been discovered in highly diverse wild, primitive, and cultivated species of tuber-bearing potatoes (Solanum L. section Petota Dumort.) and introgressed into the elite potato cultivars by hybridization and transgenic complementation. Unfortunately, even the most resistant potato varieties have been overcome by LB due to the arrival of new pathogen strains and their rapid evolution. Therefore, novel sources for germplasm enhancement comprising the broad-spectrum Rpi genes are in high demand with breeders who aim to provide durable LB resistance. The Genbank of the N.I. Vavilov Institute of Plant Genetic Resources (VIR) in St. Petersburg harbors one of the world’s largest collections of potato and potato relatives. In this study, LB resistance was evaluated in a core selection representing 20 species of seven Petota series according to the Hawkes (1990) classification: Bulbocastana (Rydb.) Hawkes, Demissa Buk., Longipedicellata Buk., Maglia Bitt., Pinnatisecta (Rydb.) Hawkes, Tuberosa (Rydb.) Hawkes (wild and cultivated species), and Yungasensa Corr. LB resistance was assessed in 96 accessions representing 18 species in the laboratory test with detached leaves using a highly virulent and aggressive isolate of P. infestans. The Petota species notably differed in their LB resistance: S. bulbocastanum Dun., S. demissum Lindl., S. cardiophyllum Lindl., and S. berthaultii Hawkes stood out at a high frequency of resistant accessions (7–9 points on a 9-point scale). Well-established specific SCAR markers of ten Rpi genes—Rpi-R1, Rpi-R2/Rpi-blb3, Rpi-R3a, Rpi-R3b, Rpi-R8, Rpi-blb1/Rpi-sto1, Rpi-blb2, and Rpi-vnt1—were used to mine 117 accessions representing 20 species from seven Petota series. In particular, our evidence confirmed the diverse Rpi gene location in two American continents. The structural homologs of the Rpi-R2, Rpi-R3a, Rpi-R3b, and Rpi-R8 genes were found in the North American species other than S. demissum, the species that was the original source of these genes for early potato breeding, and in some cases, in the South American Tuberosa species. The Rpi-blb1/Rpi-sto1 orthologs from S. bulbocastanum and S. stoloniferum Schlechtd et Bché were restricted to genome B in the Mesoamerican series Bulbocastana, Pinnatisecta, and Longipedicellata. The structural homologs of the Rpi-vnt1 gene that were initially identified in the South American species S. venturii Hawkes and Hjert. were reported, for the first time, in the North American series of Petota species.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: General Discussion and Conclusionmentioning

confidence: 99%

Section: General Discussion and Conclusionmentioning

confidence: 99%

Section: General Discussion and Conclusionmentioning

confidence: 99%

See 3 more Smart Citations

Diversity of Late Blight Resistance Genes in the VIR Potato Collection

Рогозина

Gurina

Chalaya

et al. 2023

Plants

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Specifics of Transfer of DNA Markers of Wild Allotetraploid Potato Species Solanum stoloniferum to Backcross Progenies Depending on Their Subgenomic Location and Used Schemes of Introgression

Yermishin,

Levy,

Ageeva

et al. 2023

Russ J Genet

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Peculiarities of Transfer of DNA Markers of Wild Allotetraploid Potato Species <i>Solanum stoloniferum</i> to Backcross Progenies Depending on Their Subgenomic Location and Used Schemes of Introgression

Yermishin,

Levy,

Ageeva

et al. 2023

Genetika

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Genomic difference between wild allotetraploid potato species Solanum stoloniferum Schltdl et Bouchet (genome AABB) and S. tuberosum L. (AAAA) is one of the factors hampering its use in breeding. However, there is practically no information on valuable genes of this species located in the subgenome B and on the way of their transfer into the genome A of cultivated potatoes. The objectives of this research were to identify subgenomic location of a set of S. stoloniferum genes using an original approach based on a difference of inheritance of DNA markers of the genes in backcross BC1 of chromosome doubled triploid hybrids (6x, AAAABB) to 4х S. tuberosum dependent on belonging to A or B subgenome; to study their inheritance in BC2 and BC3 in the framework of four introgression schemes and marker assisted selection of the gene Rpi-sto1. The markers of late blight (LB) resistance genes Rpi-sto1, R3b, R2, potato virus Y (PVY) resistance genes Rysto, Ryadg were located on the subgenome B and the marker of PVY resistance gene Rychc – on the subgenome A. We observed an appearance of unexpected sporadic hybrids free of markers in BC1 that may be explained by rare cases of homeological recombination of A and B subgenome chromosomes. The segregation of the markers in BC2 (close to 1 : 1) matched in general to that expected in the case of random transfer of the corresponding chromosomes of the subgenome B. Some promising for breeding hybrids have been selected in BC3 having the marker of the gene Rpi-sto1.

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Polymorphisms and Evolution of Solanum bulbocastanum Genes for Broad-Spectrum Resistance to Phytophthora infestans

Cited by 4 publications

References 24 publications

Diversity of Late Blight Resistance Genes in the VIR Potato Collection

Diversity of Late Blight Resistance Genes in the VIR Potato Collection

Specifics of Transfer of DNA Markers of Wild Allotetraploid Potato Species Solanum stoloniferum to Backcross Progenies Depending on Their Subgenomic Location and Used Schemes of Introgression

Peculiarities of Transfer of DNA Markers of Wild Allotetraploid Potato Species <i>Solanum stoloniferum</i> to Backcross Progenies Depending on Their Subgenomic Location and Used Schemes of Introgression

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