Polymorphisms and Functional Analysis of the Intact Human Papillomavirus16 E2 Gene

Ekalaksananan, Tipaya; Jungpol, Watcharapol; Prasitthimay, Chuthamas; Wongjampa, Weerayut; Kongyingyoes, Bunkerd; Pientong, Chamsai

doi:10.7314/apjcp.2014.15.23.10255

Cited by 4 publications

(2 citation statements)

References 35 publications

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“…In a great proportion of HPV-infected patients, HPV E2 restrains E6 and E7 gene transcription, which aids the regulation of cellular proliferation (28). Cleavage of the E2 gene increased HPV E6 and E7 gene expression, which disrupts the cell cycle and leads to aberrant cellular proliferation (29)(30)(31). The HPV E6 and E7 genes usually integrate into the host cell genome and require longer incubation periods for the viral DNA replication and recombination to produce a variety of genetic changes in the viral and human genome (32,33).…”

Section: Discussionmentioning

confidence: 99%

Integration sites of human papillomavirus 18 in esophageal cancer samples

Yan

Sun

et al. 2018

Oncol Lett

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Abstract. To investigate the association between human papillomavirus (HPV) infection and esophageal cancer, genomic DNA was isolated from 189 samples obtained from patients with esophageal carcinoma, and HPV DNA was identified using the polymerase chain reaction (PCR) with the following specific primers: My09/11 for HPV L1 and HPV18 E6 for HPV18. The HPV18 gene products were sequenced to identify the HPV genotype and the HPV18 integration site was verified using PCR amplification of papillomavirus oncogene transcripts. HPV18 oncogene transcript products were ligated into a pMD-18T plasmid vector and sequenced to confirm the physical location of HPV18 integration. Of the 189 samples, 168 were positive for HPV, of which 33 were positive for HPV18. The sequencing analysis identified two HPV18 E6-positive samples containing one mutation and two samples containing two mutations in the viral DNA. In total ~600 bp of the HPV18 oncogene transcript was detected in three esophageal cancer samples. Sequence analysis revealed that, in two patients, the HPV18 infection was integrated into human chromosome 5, whereas in the remaining sample the virus was integrated into human chromosome 2. The high prevalence of HPV18 infection suggested that HPV18 infection is a pathogenic factor in esophageal carcinoma progression. The integration of HPV18 DNA into the host cell genome suggests that persistent HPV infection has a role in esophageal epithelial cell malignant transformation

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Section: Discussionmentioning

confidence: 99%

Integration sites of human papillomavirus 18 in esophageal cancer samples

Yan

Sun

et al. 2018

Oncol Lett

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“…HPV protein E2 displays independent complex functions regarding the transcription and may be able to modulate host cells with respect to the viral replication cycle [48][49][50].…”

Section: E1 and E2 Proteins As Therapeutic Targetsmentioning

confidence: 99%

E1 and E2 Viral Proteins as Therapeutic Targets for Development of Antiviral Agents

Saucedo-Mendiola¹,

Ríos-Bañuelos²,

Vázquez-Vázquez³

et al. 2020

Viruses and Viral Infections in Developing Countries

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The importance of studying the human papillomavirus (HPV) is because it is a disease that relies on 14 HPV types classified as carcinogenic high risk and that contributes to cervical cancers affecting approximately 527,600 women yearly and causing 265,387 deaths yearly, being the second mortality cause for women globally. In Mexico, 13.9% of demises are due to cervical uterine cancer (CUCA). The challenges for a vaccine that may prevent HPV occurrence are an active field for scientists with significant advances but still undergoing for a full cure to this disease. In this work, latest research trends to treat HPV are analyzed, and by means of molecular coupling analysis, a modeling and simulation process to predict interactions of leader molecules with the target for synthetic elaboration of a possible therapeutic treatment is developed. One of the main topics discussed in this chapter relates to new drug design for HPV treatment, which is related to the inhibitors of protein-protein interactions and in the protein drugs. Regarding HPV therapy development, a group of small molecules has been identified using high-performance sieving capable of interrupting HPV16 E1-E2 interaction, which helps avoid viral replication. Some of these compounds displayed nanomolar affinities and high specificity.

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