Polymorphisms in CSN3, CSN2 and LGB Genes and Their Relation to Milk Production in Dairy Cattle in the Czech Republic

Čítek, J.; Hanusová, L.; Lískovcová, Lucie; Samková, Eva; Hanuš, Oto; Hasoňová, Lucie; Křížová, Zuzana; Večerek, Libor

doi:10.11118/actaun201967010019

Cited by 11 publications

(2 citation statements)

References 12 publications

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“…A significant association between genotype CSN3 and alcohol stability (AS) was found (P<0.05). As reported by Čítek et al (16) , CSN3, CSN2, and LGB polymorphisms were associated with milk yields and fat and protein production for selected cattle breeds in the Czech Republic. Although the polymorphisms of the kappa-CN gene have been studied, little information is available on how they affect milk production in crossbred dairy cows.…”

Section: Introductionsupporting

confidence: 52%

Association of Kappa casein gene polymorphism with milk production traits in crossbred dairy cows

et al. 2023

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Milk’s qualitative and technological properties are greatly affected by genetic polymorphisms in the kappa-casein gene, and their polymorphisms may serve as informative markers of yield and composition. Thus, the objective of this study was to detect kappa-casein (kappa-CN) gene polymorphisms and their association with milk production traits in crossbred dairy cows. One hundred healthy crossbred (Friesian x Jenoubi) dairy animals between three and five years old were sampled for blood and milk during their first lactation. The genomic DNA was extracted from whole blood, and restriction fragment length polymorphism (RFLP-PCR) was used to determine the genotype of the kappa-CN gene. As a consequence of the restriction digestion of this fragment with Hind III, it showed three different restriction patterns: BB (453 base pairs uncut), AB (453, 206, and 225 base pairs), and AA (206 and 225 base pairs). Based on genetic diversity, the AB genotype was the most predominant (n = 67), with a frequency of 0.67. A variant genotype of the kappa-CN gene was associated with milk production traits in crossbred dairy cows. Animals with the AA variant produced a higher milk yield and a higher percentage of fat, casein, protein, and solids not fat (SNF) (P≤0.05) (1.397kg, 0.75%, 0.31%, 0.27%, and 0.68%, respectively) than those with the BB variant. A logistic regression analysis confirmed that the kappa-CN genotypes increase milk yield and casein content. Therefore, genetic variants of the kappa-CN gene could be used as genetic markers for improving milk production traits in dairy cattle.

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Section: Introductionsupporting

confidence: 52%

Association of Kappa casein gene polymorphism with milk production traits in crossbred dairy cows

et al. 2023

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“…In recent years, the genetic polymorphisms of the CSN2 gene have received considerable attention in the animal breeding and dairy industry due to their correlation with milk production traits, quality, and β-casomorphins (BCMs) (Čítek et al, 2019;Nuhriawangsa et al, 2021;Pedrosa et al, 2021). CSN2 gene polymorphisms in the Bos genus have 12 known genetic variants; these are A1, A2, A3, B, C, D, E, F, G, H1, H2, and I, of which variants A1 and A2 are predominant and considered an ancestor type (Antonopoulos et al, 2021;Cieślińska et al, 2019;Gai et al, 2021;Sebastiani et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Genotyping, physicochemical characterization, and protein isoform quantification of β-casein A2 milk in chinese simmental and Angus cattle

Prabakusuma¹,

Aleryani

2022

Emir J Food Agric

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The recent findings on β-casein (β-CN) A2 cattle’s milk variant have triggered global research interest, as it is claimed to be a safer choice for human health. Only a few studies have been performed to investigate the presence of β-CN A1 and A2 alleles of the CSN2 gene in dual-purpose Simmental and Angus cattle in Yunnan province, China. This present study aimed to genotype cattle producing A2 milk, predict the physicochemical properties, and quantify the isoform concentration of β-CN A2 protein. Blood samples were collected from 286 cattle (201 Simmental and 85 Angus breeds). Genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) coupled with the sequencing method. Physicochemical properties were predicted using several webbased bioinformatics tools. A sandwich enzyme-linked immunosorbent assay (ELISA) was used to measure β-CN A2 protein isoform concentration in fresh A2 milk. Allele frequencies of β-CN A2 were more dominant in Simmental (0.642) and Angus (0.835) than A1. The β-CN A1A2 genotype was most frequent in Simmental (44.8%), while A2A2 genotype was primarily found in Angus (70.6%). The predicted monoisotopic mass, molecular weight, isoelectric point (pI), net charge at pH 7, total number of atoms, instability index, and grand average of hydropathicity (GRAVY) of β-CN A2 were 23,567.23 Da, 23,582.30 Da, 5.24, –6.5, 3,357, 96.53, and –0.355, respectively. The β-CN A2 protein isoform concentration in Angus (17.8 ng/ml) was higher than in Simmental (16.7 ng/ml). Identifying β-CN A2 milk provides new insights for screening favorable alleles and establishing breeding programs based on marker-assisted selection.

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Dairy productivity of Holstein cattle with different genotypes of the β-lactoglobulin gene (BLG)

Safina,

Shakirov,

Gaynutdinova

et al. 2024

BIO Web Conf.

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The article covers the study of the effect of β-lactoglobulin gene polymorphism on daity productivity, qualitative composition of milk and lactation activity of Holstein cows. The work was carried out on 146 animals in the Lenin Breeding Farm Integrated Agricultural Production Center (IAPC) of the Republic of Tatarstan. DNA genotyping by the locus of the Bos taurus BLG-Hae III gene was performed by PCR-RFLP. As a result of genetic typing of cattle, 2 alleles (A – 0.651 and B – 0.349) and 3 genotypes (AA – 41.1, AB – 47.9 and BB – 11.0 %) were identified. The chi-square criterion is below the critical value (5.99; p < 0.05) and testifies that the Hardy-Weinberg genetic equilibrium is not disturbed in the population. The evaluation of dairy productivity traits showed that cows with the AB genotype have the highest milk yield and high-quality milk. The heifers with the AB genotype are characterized by the best lactation curve throughout lactation. The difference in the amount of milk received between cows with the AB genotype and animals with the AA and BB genotypes is 71.6 kg (or 9.3 %) and 95.7 kg (or 16.9 %), respectively. Cows with low milk yields (genotype AA) had the lowest lactation decline (4.34) and greater stability (99.9).

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Polymorphisms in CSN3, CSN2 and LGB Genes and Their Relation to Milk Production in Dairy Cattle in the Czech Republic

Cited by 11 publications

References 12 publications

Association of Kappa casein gene polymorphism with milk production traits in crossbred dairy cows

Association of Kappa casein gene polymorphism with milk production traits in crossbred dairy cows

Genotyping, physicochemical characterization, and protein isoform quantification of β-casein A2 milk in chinese simmental and Angus cattle

Dairy productivity of Holstein cattle with different genotypes of the β-lactoglobulin gene (BLG)

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