Polymorphisms in IS1311, an insertion sequence common toMycobacterium aviumandM. aviumsubsp.paratuberculosis, can be used to distinguish between and within these species

Whittington, Richard J.; Marsh, Ian; Choy, Edwin; Cousins, DV

doi:10.1006/mcpr.1998.0194

Cited by 103 publications

(97 citation statements)

References 16 publications

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“…The "cattle type" was commonly detected in wide host range of animals in different parts of the world (Sevilla et al 2005). Moreover, most reports refer that goats are more ofteninfected with "cattle type" (Collins et al 1990, Whittington et al 1998. The "bison type" was found to be predominant genotype infecting domestic and wild ruminants in India (Singh et al 2010).…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of Mycobacterium avium subspecies paratuberculosis in Korean black goat (Capra hircus aegagrus)

et al. 2015

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Isolation and characterization of RESUMENLa paratuberculosis, causada por Mycobacterium avium subsp. paratuberculosis (MAP), es una infección crónica e incurable del tracto intestinal de los animales. El estudio se realizó en 2012, usando muestras procedentes de 491 cabras negras coreanas, menores de 2 años, procedentes de un rebaño caprino de la región Sur de Corea. Los animales se evaluaron para detectar presencia de paratuberculosis. Los métodos utilizados para investigar las infecciones por MAP fueron ensayo por inmunoabsorción ligado a enzimas y cultivos microbiológicos. De las 491 cabras de 100 rebaños seleccionados, 4 (0,8%) y 3 (0,6%) dieron resultados positivos para MAP en la prueba de ELISA y el estudio microbiológico de coprocultivo, respectivamente. Las colonias aisladas fueron confirmadas como MAP por IS900-PCR y su tipificación se realizó por medio del ensayo de IS1311 PCR-REA. Los resultados revelaron una baja tasa de prevalencia de infecciones por MAP de cepas de "tipo bovino" y "tipo bisonte".Palabras claves: cabra, Mycobacterium avium subsp. paratuberculosis, cultivo. SUMMARYParatuberculosis, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic incurable infection of intestinal tract of animals. The study took place in 2012, on 491 Korean black goats, older than 2 years, from goat herds located in Southern region, Korea. The animals were sampled and tested for paratuberculosis. Enzyme-linked immunosorbent assay and microbiological culture were used to investigate MAP infection. Among the 491 goats from 100 herds screened, 4 (0.8%) and 3 (0.6%) goats were defined positive for MAP by ELISA and faecal culture, respectively. Isolated colonies were confirmed as MAP by IS900-PCR, and genotyped by IS1311 PCR-REA assays. The results revealed MAP infection at a low prevalence rate with "cattle type" and "bison type" genotypes.

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Section: Resultsmentioning

confidence: 99%

Isolation and characterization of Mycobacterium avium subspecies paratuberculosis in Korean black goat (Capra hircus aegagrus)

et al. 2015

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“…The previously described primers M56 (59-GCGTGAGGCTCT-GTGGTGAA-39) and M94 (59-CAGCGATCGTCGA-CAGTGTG-39) were used to amplify a region of the IS1311 insertion sequence (Whittington et al 1998, Marsh et al 1999. Briefly, a reaction volume of 50 ml containing 5 ml of the DNA sample, 4 ml of each primer (10 pmol/ ml), 22.6 ml of water, 4 ml dNTPs (10mM), 5 ml MgCl2 (25 mM), 5 ml PCR buffer II (103), and 0.4 ml Taq Gold polymerase (5 U/ml) was used.…”

Section: Typingmentioning

confidence: 99%

Paratuberculosis or avian tuberculosis in red deer with chronic diarrhea?

et al. 2014

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SUMMARYMycobacterium avium subsp. paratuberculosis (Map) is the etiological agent of paratuberculosis. In Chile, information about Map isolation from both domestic ruminant and wildlife species has been accumulating, but it has extended to other species. In Chile, deer farming activity has been focused in hunting and meat production. No paratuberculosis surveillance has been reported for these source herds. In the present study, chronic diarrhea and poor body condition in some animals belonging to a deer farm was informed. Four adult affected hinds were euthanized under suspicion of paratuberculosis. In all animals macroscopic and microscopic lesions consistent with paratuberculosis were observed. Map-positive culture results from faecal and tissue samples, complemented with positive-PCR-results from fixed tissues are informed. Evidence has been found on cattle and deer populations being connected since molecular characterization of the Map isolated from deer lack of variation between the cattle control strain. The latter could indicate that both species share the same bacteria, suggesting there is interspecies transmission. Complementary diagnostic methods were accurate to diagnose paratuberculosis and to differentiate the clinical deer case from other mycobacterial infection. The epidemiological findings suggest that the infection in the deer farm could be transmitted from a cattle herd, making this the first reported case of paratuberculosis in deer farm in Chile.Key words: deer, histopathology, paratuberculosis, PCR. RESUMENMycobacterium avium subsp. paratuberculosis (Map) es el agente etiológico de la patuberculosis. En Chile, antecedentes del aislamiento de Map tanto en rumiantes domésticos como en especies de vida silvestre se han ido acumulando a través del tiempo, sin embargo, esto tiene que estar afectando también a otras especies. En Chile, la crianza de ciervos se ha focalizado en dos objetivos: cotos de caza y producción de carnes exóticas, no existiendo un programa de vigilancia ni siendo reportada esta enfermedad en rebaños de este tipo. Este trabajo reporta la presencia de diarrea crónica y pobre condición corporal en algunos animales pertenecientes a una granja de ciervos donde se sospechó la presencia de paratuberculosis. Cuatro ciervos adultos fueron eutanaziados bajo esta sospecha. Todos los animales presentaron lesiones macro y microscópicas compatibles con paratuberculosis. Resultados positivos al cultivo bacteriológico a partir de heces y muestras de tejido, además de PCR a partir de tejido fijado evidencian la infección. De acuerdo a los resultados de caracterización molecular de las cepas aisladas de los ciervos que no muestran variación entre cepas control de bovino, se evidencia que ambas poblaciones estarían conectadas. Complementariamente, los métodos diagnósticos utilizados fueron muy precisos para diagnosticar paratuberculosis y para diferenciar casos clínicos en ciervos de otras micobacteriosis. Las características epidemiológicas descritas en este estudio sugieren que la infecció...

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“…A cultura foi positiva em 2,0% das 300 amostras cultivadas. Os resultados da técnica PCR-REA sobre as estirpes isoladas, que foram identificadas como pertencentes ao tipo S (ovino), estão de acordo com os observados por outros autores (Whittington et al 1998, Marsh et al 1999, Sevilla et al 2005. Quanto à técnica de diagnóstico utilizada, os fracos resultados observados quanto às taxas de isolamento em cultura fecal, são semelhantes aos referenciados em idên-ticos estudos em ovinos (Juste et al 1988, Carrigan & Seaman 1990, Collins et al 1990, 1993b, Shulaw et al 1993, mas inferiores a outros documentados (Adúriz et al 1995, Garrido 2002.…”

Section: Discussionunclassified

Comparação de duas técnicas de isolamento do Mycobacterium avium subsp. paratuberculosis em amostras de fezes de ovinos com suspeita clínica de paratuberculose

et al. 2009

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A paratuberculose é uma enterite crônica granulomatosa causada por Mycobacterium avium subsp. paratuberculosis que afeta principalmente os ruminantes. A cultura de bactérias a partir de amostras de fezes e tecidos constitui um dos métodos mais eficazes de diagnóstico, sendo ainda o único método disponível para obtenção de isolamentos e estirpes de micobactérias. Contudo, este método apresenta baixa sensibilidade e requer meses de incubação antes do crescimento de colônias. Neste estudo, utilizou-se a cultura fecal como método de diagnóstico em ovinos de diferentes raças portuguesas, com sinais compatíveis com a doença. Fez-se ainda a comparação entre os meios de cultura Löwenstein Jensen® com micobactina® J e o de Middlebrook® 7H11 com OADC®, utilizados no isolamento da bactéria. As percentagens de isolamento em cada um os meios foram de 2,0% (6/300) para Löwenstein Jensen® com micobactina J e 1,0% (3/300) para Middlebrook® 7H11/OADC. As três amostras positivas no meio de Middlebrook® 7H11/OADC também foram positivas no meio de Löwenstein Jensen® com micobactina J e nenhuma foi somente positiva no meio de Middlebrook® 7H11/OADC. Os resultados deste estudo sugerem que o meio de Löwenstein-Jensen® com micobactina® J é mais efetivo para a obtenção de estirpes ovinas em Portugal.

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Polymorphisms in IS1311, an insertion sequence common toMycobacterium aviumandM. aviumsubsp.paratuberculosis, can be used to distinguish between and within these species

Cited by 103 publications

References 16 publications

Isolation and characterization of Mycobacterium avium subspecies paratuberculosis in Korean black goat (Capra hircus aegagrus)

Isolation and characterization of Mycobacterium avium subspecies paratuberculosis in Korean black goat (Capra hircus aegagrus)

Paratuberculosis or avian tuberculosis in red deer with chronic diarrhea?

Comparação de duas técnicas de isolamento do Mycobacterium avium subsp. paratuberculosis em amostras de fezes de ovinos com suspeita clínica de paratuberculose

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