Polymorphisms in miRNA genes play roles in the initiation and development of cervical cancer

Abstract

MicroRNA deregulation is crucial for cancer development. Studies showed that polymorphisms in miRNA genes could affect miRNA expression, which might be associated with cancer development. In the current study, we investigated the association of seven single nucleotide polymorphisms (SNPs) in seven miRNA genes with the initiation and development of cervical cancer in a Chinese Han population. The SNPs of 358 cervical intraepithelial neoplasia (CIN) patients, 547 cervical cancer patients and 567 healthy individuals were genotyped using TaqMan assays. Moreover, we evaluated the association of the seven SNPs with the different stages of cervical cancer. Our results showed that rs4636297 in miR-126 was associated with susceptibility to CIN and cervical cancer (P=0.019 and 0.019, respectively) and that the T allele was associated with a higher risk of CIN (OR=1.334, 95% CI: 1.049-1.698) and cervical cancer (OR=1.296, 95% CI: 1.044-1.609). Similarly, rs11614913 in miR-125a was associated with CIN and cervical cancer (P=0.025 and 0.015, respectively), and the T allele might be the protective factor for CIN (OR=0.807, 95% CI: 0.669-0.974) and cervical cancer (OR=0.814, 95% CI: 0.689-0.961). Our results indicated that rs4636297 in miR-126 and rs11614913 in miR-196a2 play an important role only in the initiation of cervical cancer not in the development of CIN to cervical cancer.

The Association between Five Genetic Variants in MicroRNAs (rs2910164, rs11614913, rs3746444, rs11134527, and rs531564) and Cervical Cancer Risk: A Meta-Analysis

“…The restoration of miR-218 significantly inhibited the proliferation, migration, and invasion of cancer cells [37]. Laminin-5 β3 (LAMB3) has been verified as a transcriptional target of miR-218 [38], and the expression of LAMB3 is increased in the presence of the HPV-16 E6 oncoprotein, and this effect is mediated by miR-218 [19]. The miR-196a2 is located on the human chromosome 12q13.13.…”

“…After reading the titles and the abstracts, 235 articles were excluded, of which 61 were duplicate ones, 149 had no relation to this topic, 22 were reviews or metaanalyses, and 3 were conference abstracts. Finally, 12 eligible case-control studies were included in our meta-analysis (Table 1) [10][11][12][13][14][15][16][17][18][19][20][21].…”

“…Recently, though a number of studies have been conducted to investigate the association of miR-124 G>C (rs531564), miR-218 A>G (rs11134527), miR-146a G>C (rs2910164), miR-196a2 T>C (rs11614913), and miR-499 A>G (rs3746444) polymorphisms with cervical cancer [2,[11][12][13][14][15][16][17][18][19][20], the results were inconclusive. Therefore, a metaanalysis was performed to evaluate the association between these five SNPs and the risk of cervical cancer.…”

The associations and roles of microRNA single-nucleotide polymorphisms in cervical cancer

“…Yan et al . reported that rs4636297 in miR-126, which was located 12 bp downstream of the pre-miR-126 sequence, was associated with CIN and cervical cancer in a Chinese Han population 66 . The results of the study by Yan et al .…”

“…The results of the study by Yan et al . indicated that the T allele confers a higher risk of developing CIN and cervical cancer 66 . The association of the SNP rs4636297 with cervical cancer may be because the SNP is related to Drosha's recognition and cleavage of pri-miRNA 34 .…”