Polymorphisms in the promoter region of<i>myostatin</i>gene are associated with carcass traits in pigs

Tu, Po-An; Lo, L. L.; Chen, Y.-C.; Hsu, Ching‐Chi; Shiau, Jen-Wen; Lin, Eric S.; Lin, Rui; Wang, P.-H.

doi:10.1111/jbg.12053

Cited by 17 publications

(11 citation statements)

References 17 publications

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“…However, despite many generations of selection for lean growth in pigs no such mutations have been reported previously. Associations between polymorphisms within the porcine MSTN gene and production traits have been shown in small-scale studies [27–29]. Further, a genome-wide association study in a sire line Large White population (related to the animals in the current study) detected SNPs on SSC 15 significantly associated with rib fat between 81.1 and 87.8 Mbp [30].…”

Section: Discussionmentioning

confidence: 75%

Balancing selection at a premature stop mutation in themyostatingene underlies a recessive leg weakness syndrome in pigs

Matika

Robledo

Pong‐Wong

et al. 2018

Preprint

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12Balancing selection provides a plausible explanation for the maintenance of deleterious 13 alleles at moderate frequency in livestock, including lethal recessives exhibiting 14 heterozygous advantage in carriers. In the current study, a leg weakness syndrome 15 causing mortality of piglets in a commercial line showed monogenic recessive 16 inheritance, and a region on chromosome 15 associated with the syndrome was identified 17 by homozygosity mapping. Whole genome resequencing of cases and controls identified 18 a mutation coding for a premature stop codon within exon 3 of the porcine Myostatin 19 (MSTN) gene, similar to those causing a double-muscling phenotype observed in several 20 mammalian species. The MSTN mutation was in Hardy-Weinberg equilibrium in the 21 population at birth, but significantly distorted amongst animals still in the herd at 110 kg, 22 due to an absence of homozygous mutant genotypes. In heterozygous form, the MSTN 23 mutation was associated with a major increase in muscle depth and decrease in fat depth, 24 suggesting that the deleterious allele was maintained at moderate frequency due to 2 25 heterozygous advantage. Knockout of the porcine MSTN by gene editing has previously 26 been linked to problems of low piglet survival and lameness. This MSTN mutation is an 27 example of putative balancing selection in livestock, providing a plausible explanation 28 for the lack of disrupting MSTN mutations in pigs despite many generations of selection 29 for lean growth. 30 Introduction 32 Leg weakness is heterogeneous condition causing lameness in pigs, and has negative 33 impacts on both animal welfare and productivity [1, 2]. Significant heritability estimates 34 have been reported for leg weakness traits [reviewed in 3], with moderate to high 35 estimates in certain pig breeds, e.g. h 2 = 0.45 in Landrace [4]. Several quantitative trait 36 loci (QTL) have been identified for these traits, albeit they are generally not consistent 37 across studies and breeds [5-8], which may be partly due to the heterogeneity of this 38 condition. Interestingly, significant genetic correlations between leg weakness and other 39 production traits (such as growth and muscle depth) have been detected [4]. Further, in a 40 divergent selection experiment in Duroc lines, selection for high leg weakness was 41 associated with a significant increase in muscle length and weight [9]. Taken together, 42 these results suggest a degree of antagonistic genetic relationship between leg weakness 43 and muscle growth traits in pigs, potentially explaining increases in the syndrome 44 observed with intense selection for lean growth in recent decades. 45 Deleterious alleles can be maintained at relatively high frequency in commercial livestock 46 populations due to heterozygous advantage for traits under selection [10]. Examples of 47 65mass and reduced fat depth than wild type homozygotes. Therefore, we propose that the 66 MSTN mutant allele is highly deleterious in this population in homozygous form, but was 67 maintained ...

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Section: Discussionmentioning

confidence: 75%

Balancing selection at a premature stop mutation in themyostatingene underlies a recessive leg weakness syndrome in pigs

Matika

Robledo

Pong‐Wong

et al. 2018

Preprint

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“…Moreover, our results show some clues for meat quality which is a major economic feature in chicken production. RYR2 and RYR3 [45][46][47]; IL-18 [48]; FBXO5 [49]; COL1A2, COL4A2, COL6A1; COL6A2; COL4A1, and COL23A1 [50]; GDF8 [51]; HSPA5 [48]; SHISA9 [52]; all bearing strong selection signals in the YFCs, are important determinants of meat quality in domestic animals. These genes provide a foundation for understanding the meat properties of the YFCs, which would attract more concerns to investigate the detailed function roles in future studies.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide genetic structure and selection signatures for color in 10 traditional Chinese yellow-feathered chicken breeds

Huang

Otecko

Peng

et al. 2020

Preprint

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Background: Yellow-feathered chickens (YFCs) have a long history in China. They are well-known for the nutritional and commercial importance attributable to their yellow color phenotype. Currently, there is a huge paucity in knowledge of the genetic determinants responsible for phenotypic and biochemical properties of these iconic chickens. This study aimed to uncover the genetic structure and the molecular underpinnings of the YFCs trademark coloration. Results: The whole-genomes of 100 YFCs from 10 major traditional breeds and 10 Huaibei partridge chickens from China were re-sequenced. Comparative population genomics based on autosomal single nucleotide polymorphisms (SNPs) revealed three geographically based clusters among the YFCs. Compared to other Chinese indigenous chicken genomes incorporated from previous studies, a closer genetic proximity within YFC breeds than between YFC breeds and other chicken populations is evident. Through genome-wide scans for selective sweeps, we identified RALY heterogeneous nuclear ribonucleoprotein (RALY), leucine rich repeat containing G protein-coupled receptor 4 ( LGR4 ), solute carrier family 23 member 2 ( SLC23A2 ), and solute carrier family 2 member 14 ( SLC2A14 ), besides the classical beta-carotene dioxygenase 2 ( BCDO2 ), as major candidates pigment determining genes in the YFCs. Conclusion: We provide the first comprehensive genomic data of the YFCs. Our analyses show phylogeographical patterns among the YFCs and potential candidate genes giving rise to the yellow color trait of the YFCs. This study lays the foundation for further research on the genome-phenotype cross-talks that define important poultry traits and for formulating genetic breeding and conservation strategies for the YFCs.

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“…They are used to predict the potential genes associated with economic traits, the linkage disequilibrium (LD) extent between markers at the genome level, as well as the livestock genetic diversity [41,42,43,44,45]. For example, researchers have used the SNP panel to estimate the LD levels of the pig breeds in Finland and United States [41].…”

Section: Discovery Of Novel Snp (Single Nucleotide Polymorphism) Fmentioning

confidence: 99%

“…They are affected by many loci that have a wide degree of effects on phenotypic variations. For instance, muscle growth is found to be regulated by MSTN gene [4,45,113]. To estimate the LD, 351 animals in 117 sire/dam/offspring trios across four breeds of pigs (Duroc, Hampshire, Landrance, Yorkshire) using Illumina PorcineSNP60 BeadChip were used.…”

Section: Application Of Trait Selection In Farm Animals By Using Snpsmentioning

confidence: 99%

“…Two SNP markers (g.435 and g.447) have been reported to associate with controlling myostatin expression in pigs. These two SNPs, located in the promoter region of MSTN gene, are vital functional genetic markers [45]. Insulin-like-growth factor 2 ( IGF2 ) gene, located in chromosome 2 of pig, has been found to positively control skeletal muscle growth, which is discovered by using RFLP.…”

Section: Application Of Trait Selection In Farm Animals By Using Snpsmentioning

confidence: 99%

See 1 more Smart Citation

Efficient SNP Discovery by Combining Microarray and Lab-on-a-Chip Data for Animal Breeding and Selection

et al. 2015

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The genetic markers associated with economic traits have been widely explored for animal breeding. Among these markers, single-nucleotide polymorphism (SNPs) are gradually becoming a prevalent and effective evaluation tool. Since SNPs only focus on the genetic sequences of interest, it thereby reduces the evaluation time and cost. Compared to traditional approaches, SNP genotyping techniques incorporate informative genetic background, improve the breeding prediction accuracy and acquiesce breeding quality on the farm. This article therefore reviews the typical procedures of animal breeding using SNPs and the current status of related techniques. The associated SNP information and genotyping techniques, including microarray and Lab-on-a-Chip based platforms, along with their potential are highlighted. Examples in pig and poultry with different SNP loci linked to high economic trait values are given. The recommendations for utilizing SNP genotyping in nimal breeding are summarized.

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Polymorphisms in the promoter region ofmyostatingene are associated with carcass traits in pigs

Cited by 17 publications

References 17 publications

Balancing selection at a premature stop mutation in themyostatingene underlies a recessive leg weakness syndrome in pigs

Balancing selection at a premature stop mutation in themyostatingene underlies a recessive leg weakness syndrome in pigs

Genome-wide genetic structure and selection signatures for color in 10 traditional Chinese yellow-feathered chicken breeds

Efficient SNP Discovery by Combining Microarray and Lab-on-a-Chip Data for Animal Breeding and Selection

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