Polymorphisms of genes involved in polycyclic aromatic hydrocarbons’biotransformation and atherosclerosis

Marinković, Natalija; Pašalić, Daria; Potočki, Slavica

doi:10.11613/bm.2013.032

Cited by 42 publications

(35 citation statements)

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“…Polymorphisms in GSTs, which encode enzymes with detoxification functions, alter the sensitivity of an organism to inflammatory processes involved in the development of atherosclerosis (Wilson et al, 2000;Izzotti et al, 2001;Hayes et al, 2005;Marinković et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…In phase II of the biotransformation, enzymes combine hydrophilic compounds such as acetyl or glutathione, which increases the solubility of xenobiotics in water and facilitates their elimination from the body (Marinković et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…The absence of GSTM1 may enhance susceptibility to cancer due to the reduced efficiency of detoxification of carcinogens (Marinković et al, 2013;Zi et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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GSTM1 polymorphism in patients with clinical manifestations of atherosclerosis

et al. 2017

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ABSTRACT. Atherosclerosis is characterized by lesions, called atheroma or atheromatous plaques, in the inner layer of blood vessels, which block the vascular lumen and weaken the underlying tunica media. Several modifiable and non-modifiable risk factors for the development of atherosclerosis exist. The modifiable risk factors include hypertension, smoking, obesity, high LDL and low HDL cholesterol levels, sedentary lifestyle, and stress; the non-modifiable factors include diabetes mellitus, family history of hypertension and heart disease, thrombophilia, sex, age, and genetic factors. The association of polymorphisms in GST with coronary artery disease has been studied since the polymorphisms can affect enzyme activity and contribute to the onset of atherosclerosis. We analyzed polymorphisms in GSTM1 in individuals diagnosed with atherosclerosis as well as in healthy individuals (control group). The frequency of the GSTM1 present genotype in the atherosclerosis group was 1.2 times higher than that observed in the control group. We found no sex-or alcoholconsumption-dependent differences between the occurrences of the present and null genotypes. However, the GSTM1 present genotype occurred in 52

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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GSTM1 polymorphism in patients with clinical manifestations of atherosclerosis

et al. 2017

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“…Some of the genes involved are CYP1A, GST (glutathione S-transferase), Apo-E, and ENSO (Marinković et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Research Article Molecular analysis of the GSTT1 gene polymorphism in patients with clinical manifestation of atherosclerosis.

Martins¹,

Rodrigues²,

Silva³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Atherosclerosis is a chronic inflammatory disease formed by the accumulation of lipids in the innermost layer and large-caliber artery (tunica intima). This accumulation, along with platelet factors, stimulates the proliferation of muscle cells in this region. Over than 400 genes may be related to the pathology since they regulate endothelial function, coagulation, inflammation, metabolism of amino acids, lipids, and carbohydrates. Glutathione S-transferases (GST) are enzymes that catalyze the polymorphic detoxification of metabolites produced by oxidative stress within the cells, which is induced by reactive oxygen species. GSTs are one of the defense mechanisms against oxidative stress damage. Due to genetic, cultural, and environmental factors, the rate of atherosclerosis is higher; however, an early diagnosis is crucial for the prevention and treatment of several complications related to the disease. The present study aimed to analyze the frequency of GSTT1 genotypes regarding the presence or absence of the polymorphism in patients with clinical manifestation of atherosclerosis. We collected 200 samples of peripheral blood of patients with the previous diagnosis of atherosclerosis based on clinical examination and imaging, and 100 samples of peripheral blood to compose the control group of patients without clinical manifestation of atherosclerosis. The polymorphism was assessed by PCR and analyzed on the agarose gel stained with 2.0% ethidium bromide. The frequency of the GSTT1 gene polymorphism was compared using the chi-square test (P < 0.05) and the G-test. In the case group, we detected 85.5% of patients with the GSTT1 genotype present and 14.5% of patients with the null genotype. A significant difference was observed between groups (case vs control) for the presence of the GSTT1 polymorphism. According to the analysis of the variable alcohol consumption, we found that in the case group the presence of the GSTT1 gene was higher in individuals who reported not drinking alcohol. In this study, the presence of the GSTT1 gene polymorphism in male patients with atherosclerosis was 1.5 times higher when compared to female patients. Regarding the variable time of smoking, we found that this genotype was more frequent in smokers for both case and control groups.

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“…Persistent organic compounds like polycyclic aromatic hydrocarbons (PAHs) can affect the expression of cytochrome P450 (CYP) and glutathione S-transferases (GST) genes (3). PAHs are environmental pollutants produced by fossil fuel combustion, industrial and natural emission, and many other sources (4).…”

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confidence: 99%

Genetic polymorphisms of the CYP1A1, GSTM1, and GSTT1 enzymes and their influence on cardiovascular risk and lipid profile in people who live near a natural gas plant

Pašalić

Marinković²

2017

Archives of Industrial Hygiene and Toxicology

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The aim of this cross-sectional study was to see whether genetic polymorphisms of the enzymes CYP1A1, GSTM1, and GSTT1 are associated with higher risk of coronary artery disease (CAD) and whether they affect lipid profile in 252 subjects living near a natural gas plant, who are likely to be exposed to polycyclic aromatic hydrocarbons (PAHs). Fasting serum concentrations of biochemical parameters were determined with standard methods. Genetic polymorphisms of CYP 1A1 rs4646903, rs1048943, rs4986883, and rs1799814 were genotyped with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFPL), while GSTM1 and GSTT1 deletions were detected with multiplex PCR. Cardiovascular risk was assessed with Framingham risk score, and the subjects divided in two groups: >10% risk and ≤10% risk. The two groups did not differ in the genotype frequencies. MANCOVA analysis, which included lipid parameters, glucose, and BMI with sex, age, hypertension and smoking status as covariates, showed a significant difference between the GSTT1*0 and GSTT1*1 allele carriers (p=0.001). UNIANCOVA with same covariates showed that total cholesterol and triglyceride levels were significantly higher in GSTT1*1 allele carriers than in GSTT1*0 carriers (p<0.001 and p=0.006, respectively). Our findings suggest that CYP1A1, GSTM1, and GSTT1 polymorphisms are not associated with the higher risk of CAD, but that GSTT1 affects lipid profile.

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Polymorphisms of genes involved in polycyclic aromatic hydrocarbons’biotransformation and atherosclerosis

Cited by 42 publications

References 64 publications

GSTM1 polymorphism in patients with clinical manifestations of atherosclerosis

GSTM1 polymorphism in patients with clinical manifestations of atherosclerosis

Research Article Molecular analysis of the GSTT1 gene polymorphism in patients with clinical manifestation of atherosclerosis.

Genetic polymorphisms of the CYP1A1, GSTM1, and GSTT1 enzymes and their influence on cardiovascular risk and lipid profile in people who live near a natural gas plant

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