Polymorphisms of RDH16 and VEGFR1 influence M. trapezius steatosis in Japanese Black carcass

Abstract

The exact cause of steatosis, one of defects in Japanese beef carcasses, has not been elucidated to date, because it is very difficult to diagnose cyclopedically with certain reproducibility due to the bias in the outbreak. Therefore, the objective of this study was to assess the influence of polymorphisms in retinol dehydrogenase 16 (RDH16), myoferlin (MYOF) and vascular endothelial growth factor receptors 1 and 2 (VEGFR1, VEGFR2) on carcass-graded Musculus trapezius steatosis. For logistic regression analysis, 646 carcasses shipped from 29 farms in Miyazaki, Japan, were used. The GG genotype in RDH16 showed significant odds ratios against AA and AG. In VEGFR1, CT had a significant odds ratio against CC. After evaluating for interaction, highly significant odds ratios were observed in the combinations that included the GG risk genotype in RDH16. It is noteworthy that there was no steatosis in the combination GG (RDH16) and CC (VEGFR1). It may be concluded that there is a possibility that steatosis can be suppressed by the CC genotype in VEGFR1. The current study revealed the influence of genetic polymorphisms on M. trapezius steatosis that had not been reported until now, and may help elucidate the cause of steatosis.

Fat depot-specific differences of macrophage infiltration and cellular senescence in obese bovine adipose tissues

“…In fattening Wagyu cattle, obesity-associated adipose tissue disorders, called “fat necrosis” and “muscle inflammation”, occasionally develop during fattening periods [ 18 , 34 ]. The histological analysis of bovine fat necrosis shows an increasing fibrous area within visceral adipose tissue [ 20 ].…”

“…These results suggest that “fat necrosis” of bovine visceral adipose tissue might be affected by the severe inflammatory condition induced by obesity. “Muscle inflammation” is generally observed at the musculus trapezius and longissimus dorsi of fattening Wagyu cattle; these musculatures contain the largest amount of intramuscular adipose tissue among bovine muscles [ 18 , 19 ]. The histological analysis of bovine “muscle inflammation” is characterized by muscular steatosis [ 4 , 33 ].…”

Breed differences in macrophage infiltration and senescence state in adipose tissues of Wagyu and Holsteins

“…These results indicate that breed differences between Wagyu and Holsteins affect the macrophage infiltration and senescence state in visceral adipose tissue. In fattening Wagyu cattle, obesity‐associated adipose tissue disorders, called “fat necrosis,” occasionally develop in visceral adipose tissue (Ishida, Noda, Jomane, & Tokunaga, 2017; Oka, Iwamoto, & Tatsuda, 2015), characterized by an increase in the fibrous area within visceral adipose tissue (Ito, Miura, Ohshima, & Numakunai, 1968). Severe obese conditions accelerate macrophage infiltration and stimulate fibrosis of adipose tissue both in humans and rodents (Divoux et al., 2010; Kawanishi, Niihara, Mizokami, Yano, & Suzuki, 2013; Khan et al., 2009; Reggio, Pellegrinelli, Clement, & Tordjman, 2013).…”

Genomic Approaches Reveal Pleiotropic Effects in Crossbred Beef Cattle

“…Columns show the chromosome (BTA), the start and end location of the genomic region, the associated trait, and the direct additive genetic variance explained (%). and the Retinol Dehydrogenase 16 (RDH16) gene that is involved in retinol metabolism and seems to be involved in steatosis in Japanese Black cattle (Ishida et al, 2017). This second BTA5 region is of particular importance because of its pleiotropic effects on most of the traits under investigation.…”