Polymorphisms of IGFI contribute to the development of ischemic stroke

Abstract

Abstract. Insulin-like growth factor 1 (IFG1) is neuroprotective in animal models of focal brain ischemia and correlates with ischemic stroke (IS) outcome in the elderly. In this study, we investigated whether single nucleotide polymorphisms (SNPs) of the IFG1 gene are associated with the development and clinical features of IS in a Korean population. A total of 119 patients with IS and 289 control subjects were recruited. Stroke patients were classified into subgroups according to the scores of the National Institutes of Health Stroke Survey (NIHSS; <6 and ≥6) and the Modified Barthel Index (MBI; <60 and ≥60). Among the SNPs of the IFG1 gene, five SNPs were selected and analyzed by direct sequencing: rs2162679 (intron), rs2195239 (intron), rs978458 (intron), rs1520220 (intron) and rs6214 (3' untranslated region; 3'UTR). Multiple logistic regression models were conducted to analyze genetic data. SNPStats, SNPAnalyzer Pro and Helixtree programs were used to calculate odds ratios (ORs), 95% confidence intervals (CIs) and p-values. Two SNPs, rs2162679 and rs6214, were associated with the development of IS. After Bonferroni correction (p c ), the A and G alleles of rs2162679 and rs6214 had significant differences between patients with IS and the controls [rs2162679, OR (95% CI) = 1.64 (1.17-2.31), p=0.004, p c =0.02; rs6214, OR (95% CI) = 1.52 (1.12-2.07), p=0.007, p c =0.035], respectively. However, the five selected SNPs were not related to the NIHSS and MBI scores. These results suggest that IGF1 may be associated with the development of IS.

A new prognostic scale for the early prediction of ischemic stroke recovery mainly based on traditional Chinese medicine symptoms and NIHSS score: a retrospective cohort study

“…Stroke has been listed as the third leading cause of death following heart diseases and cancer in the world [ 3 , 4 , 6 ]. Ischemic stroke accounts for approximately 80 % of the total cerebrovascular diseases [ 8 ]. Therefore, the prevention and treatment of ischemic stroke has attracted a great attention from the medical profession domestically and internationally in recent years [ 9 , 10 ].…”

A comprehensive contribution of genetic variations of the insulin-like growth factor 1 signalling pathway to stroke susceptibility

“…Our previous study showed that IGF1 polymorphism (rs35767) was associated with hypertension in normal weight and obesity populations [44]. Hak et al suggested that rs6214 and rs2162697 of the IGF1 gene were related with the development of stroke [12]. Genetic studies also reported that variations at IGFBP3 (rs11977526 and rs2949837) were related to the increased blood pressure [43,45].On the other hand, genetic variation at rs6032470 had lower expression levels of IGF1R genes.…”

“…A case-control study of ischaemic stroke (IS) in a Korean population observed that common variants in IGF1 genes (rs2162679 and rs6214) exhibited protective effects on the development of IS [12]. Epidemiological studies revealed that the levels of circulating IGF1 and IGFBP3 below normal range were associated with the increased morbidity and mortality from ischaemic heart disease and stroke [13][14][15].…”

The Sequencing of the Insulin-like Growth Factor 1 and Fibulin 5 Gene Variants in the Pre and Post-menopausal Women with Stress Urinary Incontinence

“…Yang et al (18) found the carriers of rs6214 GG genotype have the risk of low appendicular skeletal muscle mass. IGF1 rs6214 variation wasalso found associated with Barrett esophagus (19) and the development of ischemic stroke (20). Because IGF1 plays a crucial role in hypothalamicpituitary-ovarian hormone-controlled metabolic processes, Zhao et al (21) studied rs6214 on age at menarchevariation in Caucasian women and detected the association.…”