Polymorphisms of the Human Low-Density Lipoproteins

Bütler, R.

doi:10.1159/000464566

Cited by 10 publications

(12 citation statements)

References 31 publications

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“…patients having received more than 20 transfusions) ; in these cases the anti-LDL antibodies are obviously of isoimmune origin. In agreement with the observations of other authors [3,9], a markedly higher frequency (about 20 times) of such antibodies was found in multitransfused patients with Cooley anemia than in found (G 34, [8]) appears to be a very rare event. Antibodies against factors of the Ag(a15c,m,t, x,y,z) system have not been encountered in the total of 931 sera of healthy persons (blood donors).…”

Section: Central Laboratory Of the Blood Transfusion Service Of The Ssupporting

confidence: 92%

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Further Observations on Human Anti-LDL Antibodies: SerologicalBehaviour, Frequency and Mode of Formation

1969

Vox Sanguinis

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Section: Central Laboratory Of the Blood Transfusion Service Of The Ssupporting

confidence: 92%

“…Five sera turned out to be monospecific, the remaining four polyspecific. Anti-Ag(x) antibodies were frequently encountered, in accordance with previous observations of many authors [3,9]. All of the antibody containing sera were found in multitransfused patients (i.e.…”

Section: Central Laboratory Of the Blood Transfusion Service Of The Ssupporting

confidence: 89%

Further Observations on Human Anti-LDL Antibodies: SerologicalBehaviour, Frequency and Mode of Formation

1969

Vox Sanguinis

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“…(The above conclusions are of course subject to the qualification that rabbit antisera may not detect subtle differences between human proteins. Such relative insensitivity of heterologous antisera has been observed with LDL allotypes (43,44).) Total plasma ApoA-I levels were similar in normal and type II and type IV hyperlipoproteinemic subjects.…”

mentioning

confidence: 58%

The Structure of Human High Density Lipoprotein and the Levels of Apolipoprotein A-I in Plasma as Determined by Radioimmunoassay

Schonfeld¹,

Pfleger²

1974

J. Clin. Invest.

209

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The major apoprotein of high density lipoprotein is apolipoprotein A-I (ApoA-I). In addition to being a structural component of this class of lipoproteins, ApoA-I also has a physiologic role as an activator of lecithin-cholesterol acyl transferase, an enzyme important in the metabolism of all lipoproteins.To measure ApoA-I content in human plasma, to assess its immunologic activity in hyperlipoproteinemia, and to carry out certain structural studies of high density lipoproteins, we have developed a double antibody radioimmunoassay. ApoA-I, isolated by gel filtration, was used to produce monospecific antisera.

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“…In contrast, intraspecies immunization preferentially elicits an immune response against allogeneic or polymorphic epitopes. An example of the latter is the Agsystem of human apoB polymorphism that was originally defined by antibodies in the sera of multitransfused individuals and multiparous women (13). Similarly, the complex apoB polymorphisms of swine were identified with antibodies generated by allogeneic immunization (14).…”

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confidence: 99%

Immunochemical evidence that human apoB differs when expressed in rodent versus human cells

Wang

Chauhan

Nguyen

et al. 2003

Journal of Lipid Research

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LDL from human apolipoprotein B-100 (apoB-100) transgenic ( HuBTg ؉ / ؉ ) mice contains more triglyceride than LDL from normolipidemic subjects. To obtain novel monoclonal antibody (MAb) probes of apoB conformation, we generated hybridomas from HuBTg ؉ / ؉ that had been immunized with LDL isolated from human plasma. One apoE-specific and four anti-apoB-100-specific hybridomas were identified. Two MAbs, 2E1 and 3D11, recognized an epitope in the amino-terminal 689 residues of apoB in native apoB-containing lipoproteins (LpBs) from human plasma or from the supernatant of human hepatoma HepG2 cells, but did not react with LpB from HuBTg ؉ / ؉ mice or LpB secreted by human apoB-100-transfected rat McArdle 7777 hepatoma cells. 2E1 reacted weakly and 3D11 reacted strongly with apoB from HuBTg ؉ / ؉ mice after SDS-PAGE. The lack of expression of the 2E1 and 3D11 epitopes on native LpB from HuBTg ؉ / ؉ mice did not solely reflect the abnormal lipid composition of murine LpB. Both epitopes were detected in all human plasma samples tested and in all human plasma LpB classes. Therefore, human apoB expressed by rodent hepatocytes or hepatoma cells appears to adopt a different conformation or undergoes different posttranslational modification than apoB expressed in human hepatocytes or hepatoma cells. -Wang, X

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Polymorphisms of the Human Low-Density Lipoproteins

Cited by 10 publications

References 31 publications

Further Observations on Human Anti-LDL Antibodies: SerologicalBehaviour, Frequency and Mode of Formation

Further Observations on Human Anti-LDL Antibodies: SerologicalBehaviour, Frequency and Mode of Formation

The Structure of Human High Density Lipoprotein and the Levels of Apolipoprotein A-I in Plasma as Determined by Radioimmunoassay

Immunochemical evidence that human apoB differs when expressed in rodent versus human cells

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