Polymorphisms of the PLAG1 and their relationships with body measurements and carcass traits of Ongole crossbred cattle

Abstract

Pleomorphic adenoma gene 1 (PLAG1) is a member of the PLAG family which is associated with body size in various cattle breeds. This study investigated the association of PLAG1 polymorphisms to carcass traits and body measurement of Ongole crossbred (PO) cattle in Kebumen. Chest girth, body length, body height, withers height, waist height, live body weight, carcass weight, and carcass percentage were carefully measured and recorded. The DNA genome extractions were originated from blood samples that was collected via jugular vein. The extracted DNA template used to amplify two PLAG1 polymorphisms target, namely, g.48308C>T and g.32212 (19 bp indel). Identification of g.48308C>T from PLAG1 was done via PCR-RFLP technique using SacII, wherein the 19 bp indel was obtained from PCR products. One-way analysis of variance and independent sample t-test were carried out to quantify the obtained data. This present study revealed variation in both g.48308C>T SNP and 19 bp indel. Three genotypes, TT, TC, and CC, were found for g.48308C>T SNP. Whilst two genotypes DD and DI genotypes were found for 19 bp indel. Non-significant differences were found between PLAG1 polymorphisms and traits of interest observed in Kebumen PO cattle population.