Polymorphisms of the prion protein gene (PRNP) in Chinese domestic sika deer (Cervus nippon hortulorum)

Identification of single-nucleotide polymorphisms of the prion protein gene in sika deer (Cervus nippon laiouanus)

“…The SNP (T→C at nucleotide 408) at codon 136 was found to be a silent mutation, which did not induce any amino acid substitutions (Fig. 3), as previously reported in studies of the sika deer inhabiting China and Japan [5,6]. The allele frequencies of that polymorphism were 59.09 and 40.91% for T and C, respectively (Table 1).…”

“…The allele frequencies of the polymorphism were 96.97 and 3.03% for A and G, respectively (Table 1). Two SNPs located at codons 136 and 226 have previously been reported in the Chinese domestic sika deer [6]. The SNP (T→C at nucleotide 408) at codon 136 was found to be a silent mutation, which did not induce any amino acid substitutions (Fig.…”

“…Therefore, we are currently unable to determine the correlation between a specific allele of the PRNP and the CWD susceptibility of sika deer. Instead, single-nucleotide polymorphisms (SNPs) of PRNP have been identified in both Chinese and Japanese domestic sika deer [5,6]. This study characterized three SNPs in the PRNP of sika deer ( Cervus nippon laiouanus ) reared in Korea, one of which had not been reported previously.…”

“…The SNPs in the sika deer PRNP have been identified in Chinese and Japanese domestic sika deer at nucleotides 408 and 676, and 63, 255 and 408, respectively [5,6]. The allele frequencies detected at codons 136 (nucleotide position 408) and 226 (nucleotide position 676) in the PRNP of Korean domestic sika deer were only slightly different from those reported in Chinese domestic sika deer [6].…”

Genetic variability of the prion protein gene (PRNP) in wild ruminants from Italy and Scotland

“…Polymorphisms at codons 95, 96 and 116 were found to have a significant influence on the susceptibility to CWD [14,15,19]. Amino acid variations at codons 100 (S→G) and 226 (E→Q) have been described in Chinese and Korean captive sikadeer ( Cervus nippon ) [11,12]. PRNP genetic variability in European wild ruminants has not been thoroughly investigated, but the identification of PRNP polymorphisms and comparing them with those in North American cervids and domestic small ruminants may provide an estimate of the susceptibility of these species to CWD and other TSEs.…”