Polymorphonuclear leukocyte functions as predictive markers for infections after organ transplantation

Egger, G.; Burda, Astrid; Hengster, Paul; Kunc, Marialuise; Margreiter, Raimund

doi:10.1111/j.1432-2277.2000.tb01050.x

Cited by 16 publications

(5 citation statements)

References 16 publications

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“…An accelerated release of newly formed PMN cells from the bone marrow is known as a common feature of the systemic response to an inflammatory stimulus (16–18). Our assumption is in line with other reports indicating that PMN migration and ROS release, rather than PMN count, reflect PMN cell activity (19–21; G. Egger, personal communication); moreover, our results are in accord with an observation that ROS release negatively correlated with PMN migration (22). PMN counts can be beneficially employed for group analysis and evaluation of group behavior (1).…”

Section: Discussionsupporting

confidence: 93%

Determinants of Pathology in Light‐irradiated Cells

Sinyakov¹,

Zhevelev²,

Avtalion³

2010

Photochem & Photobiology

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This work is devoted to further evaluation of pathological states in light-irradiated cells in terms of relationship between the level of reactive oxygen species (ROS) and polymorphonuclear neutrophil (PMN) count. Human leukocytes were isolated from 34 donors exhibiting flu-like symptoms, irradiated with a nonlaser blue light (2 and 5 mW cm(-2)) and examined for ROS production by a chemiluminescence (CL) assay. The level of ROS was measured as a basal chemiluminescence before (BCL0) and after (BCL) irradiation. In our preceding study (Sinyakov, M. et al. [2007] Photomed. Laser Surg.25, 257-263), PMN-oriented group analysis was applied to a data set divided into light-resistant and light-sensitive points, and cell responsiveness to light was suggested for prognostic evaluation of pathology. In the present work, BCL-oriented analysis was performed on the whole data set to evaluate the individual contribution of every point. BCL level and PMN count were confirmed to be independent variables. Sign-specific delta (DEL) values (DEL = BCL - BCL0) were assessed in relation to respective levels of BCL, BCL0 and PMN counts. DEL and BCL0 were found to be reliable photochemical indices of pathology. Taken together, the results obtained from group and individual analyses provide a novel outlook on the dynamics of pathological development and indicate a promising potential of the assay as a fast discriminator between the nonpathological state and different levels of pathology.

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Section: Discussionsupporting

confidence: 93%

Determinants of Pathology in Light‐irradiated Cells

Sinyakov¹,

Zhevelev²,

Avtalion³

2010

Photochem & Photobiology

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“…Our data are in good agreement with earlier studies by our working group on other topics demonstrating that the mere number of circulating PMNs does not necessarily reflect their immunological efficiency, and functional parameters are much more reliable. Before infections, we often found a low PMN readiness to migrate that coincided with elevated blood counts feigning an effective host defense [11][12][13][14][15] .…”

Section: Discussionmentioning

confidence: 99%

“…With increasing duration and degree of impaired PMN migration, the risk of infection increased in parallel, eventually resulting in the clinical manifestation of an infection. In this way, it was possible to predict impending infections [11][12][13][14][15] .…”

mentioning

confidence: 99%

Impaired Whole‐Blood Polymorphonuclear Leukocyte Migration as a Possible Predictive Marker for Infections in Preterm Premature Rupture of Membranes

Glasner

Egger

Winter

2001

Infectious Diseases in Obstetrics and Gynecology

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Objectives: Steroids, used in preterm premature rupture of membranes (pPROM), to reduce the risk of morbidity and mortality of the preterm neonate, impair the maternal polymorphonuclear leukocyte (PMN)-based immune system. In spite of combination with antibiotics, prenatal and postnatal bacterial infections of mother and child are frequent. This pilot study focuses on the influence of steroids in pPROM on maternal PMN functional capacity and subsequent infections. Methods: After opting for expectant management, eight women with pPROM and no signs of infection were treated by steroids (betamethasone 5.7 mg, i.m. every 24 hours, for three days) and antibiotic therapy with either amoxicillin and clavulanic acid, piperacillin or ampicillin i.v. up to delivery. The conventional inflammation parameters of PMN blood count and C-reactive protein (CRP) were measured daily in parallel with PMN migratory capacity towards N-formyl-methionyl-leucyI-phenylalanine stimulation and under blank conditions, estimated by a whole blood membrane filter assay. Results: In all patients PMN migration decreased during the application of steroids. Three patients showed a decrease in PMN migration below critical values and in spite of antibiotic prophylaxis acute pyelonephritis developed 2-6 days later. PMN count and CRP were not predictive of maternal infection. Conclusion: Reduced PMN function, caused by steroid treatment in pPROM, is suggested to be a reason for serious bacterial infections in spite of antibiotic prophylaxis. PMN migration reflects individual PMN defensive capacity.

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“…A simple test to determine susceptibility to invasive fungal infection could be extremely useful. Egger et al (48) evaluated neutrophil function as a predictive marker for subsequent infection. Elevated serum elastase and a decrease in whole blood chemotactic responses to the bacterial formyl peptide f‐met‐leu‐phe occurred as early as 15 days prior to the onset of sepsis in 6 of 7 patients.…”

Section: Discussionmentioning

confidence: 99%

Impaired phagocyte respiratory burst responses to opportunistic fungal pathogens in transplant recipients: in vitro effect of r‐metHuG‐CSF (Filgrastim)

Pursell

Verral

Daraiesh

et al. 2003

Transplant Infectious Dis

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Phagocyte respiratory burst capacity in response to pathogenic fungi and the in vitro effects of granulocyte colony-stimulating factor (G-CSF) were examined in 15 normal volunteers and 39 transplant recipients (4 autologous and 4 allogeneic bone marrow, 3 heart, 10 liver, 8 lung, and 10 kidney). Chemiluminescence was measured for reaction mixtures of diluted whole blood, opsonized fungi, and luminol, with and without in vitro incubation with r-metHuG-CSF (Filgrastim). Transplant patients exhibited significantly impaired respiratory burst responses to conidia and yeast compared with controls, but this was reversed with Filgrastim. Responses to hyphae were low for both groups, and G-CSF had little or no effect. There was excellent correlation between responses to fungi and responses to opsonized zymosan. In vitro respiratory burst capacity is impaired in transplant recipients. This may predict susceptibility to invasive fungal infections. G-CSF can reverse impaired phagocyte function and is of potential benefit in the prevention and/or treatment of fungal infection in transplant patients.

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Polymorphonuclear leukocyte functions as predictive markers for infections after organ transplantation

Abstract: Polymorphonuclear leukocyte functions as predictive markers for infections after organ transplantation-Abstract Infectious complications

Cited by 16 publications

References 16 publications

Determinants of Pathology in Light‐irradiated Cells

Determinants of Pathology in Light‐irradiated Cells

Impaired Whole‐Blood Polymorphonuclear Leukocyte Migration as a Possible Predictive Marker for Infections in Preterm Premature Rupture of Membranes

Impaired phagocyte respiratory burst responses to opportunistic fungal pathogens in transplant recipients: in vitro effect of r‐metHuG‐CSF (Filgrastim)

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