Polymorphonuclear Leukocyte‐Inhibitory Factor of Bordetella pertussis

Abstract

In vivo biologic effects of the polymorphonuclear leukocyte-inhibitory factor (PIF) of Bordetella pertussis were tested by using two experimentally induced. inflammatory processes in mice. The intravenous injection of a partially purified extract from phase I bacteria strongly inhibited the glycogen-induced peritoneal infiltration of polymorphonuclear leukocytes (PMN) and the Arthus reactions, whereas little inhibitory activity was found in the extract from phase III bacteria. The activity was localized in the outer membrane of phase I bacteria, as was the in vitro PIF activity, and the two activities gave the same behavior in DEAE-cellulose chromatography.Therefore the observed suppression of inflammatory processes in mice is probably due to the inhibitory action of PIF on the function of PMN in vivo.Earlier studies have shown that Bordetella pertussis vaccine renders its recipients more susceptible to a variety of bacterial (1, 3, 5, 6) and viral (13) infections. These organisms might produce an active component which depresses the host's defense mechanism. Thus Levine and Sowinsky (9) made an interesting observation that injection of pertussis vaccine inhibited the migration of monocytes into thermally coagulated, necrotized loci in the brains of experimental mice. The polymorphonuclear leukocyte-inhibitory factor (PIF), which has been shown to be produced by virulent strains of B. pertussis and to interfere with the phagocytic and chemotactic functions of human polymorphonuclear leukocytes (PMN) in vitro (4,14), may also be related to the deterioration of the host's defense. Therefore we designed experiments to see if PIF could also block the PMN-mediated inflammatory responses in vivo by the use of the experimental systems of Arthus reactions and glycogen-induced peritoneal infiltration of PMN in the mouse. The results indicate that this may indeed be the case.

Accumulation of a precursor of subunit S1 of pertussis toxin in cell envelopes of Bordetella pertussis in response to the membrane perturbant phenethyl alcohol

“…The localisation of of PT are synthesised and to elucidate the way in which PT is released into the culture medium. the histamine-sensitising activity (attributed to PT by Wardlaw and Parton, 1982) mainly in the outer membrane of B. pertussis strain Tohama (Imagawa et al, 1979) may substantiate this view. Further work is in progress to determine how other subunits…”

“…This was done as described by Imagawa et al (1979). Crude membranes were layered over discontinuous gradients of sucrose at 40, 45, 50 and 55% w/v in Tris-EDTA buffer.…”

Lymphocytosis-promoting factor of Bordetella pertussis alters mononuclear phagocyte circulation and response to inflammation

“…injection of TGB. It has been reported that phagocytic cells in animals injected with pertussis vaccine migrated poorly into a localized inflammatory site (1,13,17), and a series of experiments was performed to determine if LPF was responsible for this inhibition. The response of MNPs to an i.p.…”