Polypeptide from Chlamys farreri suppresses ultraviolet-B irradiation-induced apoptosis through restoring ER redox homeostasis, scavenging ROS generation, and suppressing the PERK-eIF2a-CHOP pathway in HaCaT cells

Zhong, Feng; Xie, Jing; Zhang, Di; Han, Yantao; Wang, Chunbo

doi:10.1016/j.jphotobiol.2015.06.016

Cited by 27 publications

(19 citation statements)

References 24 publications

(32 reference statements)

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“…40 However, activated IRE-1 could promote intracellular ROS levels given that the IRE-1-dependent activation of ASK and P38 MARK contributes to ROS generation via further activation of CHOP. 41 In this study, we proved that chemical chaperones trehalose and 4-PBA not only mitigated OGD-induced ER stress, but also suppressed the abnormal increase in intracellular ROS caused by OGD. Therefore, we think that activation of ER stress is a pathway contributing to the generation of excessive ROS caused by OGD in the SH-SY5Y cells.…”

Section: Inhibition Of Ros With Antioxidant Nac and Mitigation Of Er mentioning

confidence: 58%

“…The PERK pathway has negative regulation of intracellular ROS levels, because the activated PERK could free Nrf2 by phosphorylation of Keap1 and facilitate Nrf2 translocation into nucleus to express antioxidant proteins . However, activated IRE‐1 could promote intracellular ROS levels given that the IRE‐1‐dependent activation of ASK and P38 MARK contributes to ROS generation via further activation of CHOP . In this study, we proved that chemical chaperones trehalose and 4‐PBA not only mitigated OGD‐induced ER stress, but also suppressed the abnormal increase in intracellular ROS caused by OGD.…”

Section: Discussionmentioning

confidence: 68%

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Endoplasmic reticulum stress regulates oxygen‐glucose deprivation‐induced parthanatos in human SH‐SY5Y cells via improvement of intracellular ROS

Wang

Ding

et al. 2017

CNS Neurosci Ther

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Section: Inhibition Of Ros With Antioxidant Nac and Mitigation Of Er mentioning

confidence: 58%

Section: Discussionmentioning

confidence: 68%

Endoplasmic reticulum stress regulates oxygen‐glucose deprivation‐induced parthanatos in human SH‐SY5Y cells via improvement of intracellular ROS

Wang

Ding

et al. 2017

CNS Neurosci Ther

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“…PERK phosphorylates eukaryotic initiation factor 2 (eIF2α) to reduce the GTP-bound form (initial phase of polypeptide synthesis) [ 21 ] and allow translation of ATF4, one of the UPR-dependent signaling proteins. Other activation mechanisms involve IRE1α-dependent ASK and p38 MAPK activation, which further activate CHOP to contribute to reactive oxygen species (ROS) generation [ 22 ]. Herein, we discuss persistent ER stress and protein misfolding that initiate ROS cascades and are known to play significant roles in disease pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

Endoplasmic Reticulum Stress and Associated ROS

Zeeshan

Lee

Kim

et al. 2016

IJMS

724

505

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The endoplasmic reticulum (ER) is a fascinating network of tubules through which secretory and transmembrane proteins enter unfolded and exit as either folded or misfolded proteins, after which they are directed either toward other organelles or to degradation, respectively. The ER redox environment dictates the fate of entering proteins, and the level of redox signaling mediators modulates the level of reactive oxygen species (ROS). Accumulating evidence suggests the interrelation of ER stress and ROS with redox signaling mediators such as protein disulfide isomerase (PDI)-endoplasmic reticulum oxidoreductin (ERO)-1, glutathione (GSH)/glutathione disuphide (GSSG), NADPH oxidase 4 (Nox4), NADPH-P450 reductase (NPR), and calcium. Here, we reviewed persistent ER stress and protein misfolding-initiated ROS cascades and their significant roles in the pathogenesis of multiple human disorders, including neurodegenerative diseases, diabetes mellitus, atherosclerosis, inflammation, ischemia, and kidney and liver diseases.

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“…It is one of the major economic scallops cultured worldwide [ 1 ]. Different functional peptides derived from scallops had been extensively shown in the literature, such as the ability to protect against UV radiation [ 2 ], ACE inhibitor activity [ 2 , 3 ], and antibacterial properties [ 4 , 5 , 6 ]. Ding et al [ 7 ] have studied the protective effect of polypeptide from Chlamys farreri against UV-irradiation on dermal fibroblasts.…”

Section: Introductionmentioning

confidence: 99%

Effect of Ball Mill Treatment on the Physicochemical Properties and Digestibility of Protein Extracts Generated from Scallops (Chlamys farreri)

Chen

et al. 2018

IJMS

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The effects of ball mill treatment (0, 2, 4, 6, 8, and 10 min) on the physicochemical and digestible properties of scallops (Chlamys farreri) protein (CFP) were investigated. The CFP particle size decreased with increasing ball-milling time. The content of free sulfhydryl (SH) of CFP increased from 13.08 ± 0.25 μmol/g protein to 18.85 ± 0.24 μmol/g protein when the ball-milling time increased from 0 min to 10 min. A sharp increase of the surface hydrophobicity index (H0) from 48.53 ± 0.27 to 239.59 ± 0.37 was found when the ball-milling time increased from 0 min to 4 min. Furthermore, the foaming capacity increased from 46.08 ± 6.12% to 65.11 ± 1.05% with increasing ball-milling time from 0 min to 6 min, after which it reached a plateau. SDS-PAGE results showed that ball mill treatment did not change the primary structure of CFP. Digestible properties of BMCFP simulated gastrointestinal digestion as a function of ball mill treatment were analyzed by Tricine-SDS-PAGE and nitrogen recovery index. After 60 min of simulated human gastro digestion, nitrogen recovery index of CFP had a significant rise from 42.01 ± 0.31% to 58.78 ± 3.37% as the ball-milling time increased from 0 min to 6 min. Peptides from hydrolysates of Chlamys farreri protein (CFP) were identified by ultraperformance liquidchromatographysystem coupled to a Synapt Mass Quadrupole Time-of-Flight Mass Spectrometer (UPLC-Q-TOF-MS). After 2 h and 4 h of simulated human duodenal digestion, the number of peptides with 7–10 amino acids length increased apparently with the ball-milling time increased. This study presents an approach to investigating the effect of the ball-milling process on the physicochemical and digestible properties of CFP, which may provide valuable information on the application of CFP as an ingredient in food products.

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Polypeptide from Chlamys farreri suppresses ultraviolet-B irradiation-induced apoptosis through restoring ER redox homeostasis, scavenging ROS generation, and suppressing the PERK-eIF2a-CHOP pathway in HaCaT cells

Cited by 27 publications

References 24 publications

Endoplasmic reticulum stress regulates oxygen‐glucose deprivation‐induced parthanatos in human SH‐SY5Y cells via improvement of intracellular ROS

Endoplasmic reticulum stress regulates oxygen‐glucose deprivation‐induced parthanatos in human SH‐SY5Y cells via improvement of intracellular ROS

Endoplasmic Reticulum Stress and Associated ROS

Effect of Ball Mill Treatment on the Physicochemical Properties and Digestibility of Protein Extracts Generated from Scallops (Chlamys farreri)

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