Polyphasic approach to the taxonomy of the Rhizopus stolonifer group

Liou, Geou-Yarh; Chen, Shyue-Ru; Wei, Yuhui; Lee, Fwu-Ling; Fu, Hui-Mei; Yuan, Gwo‐Fang; Stalpers, J. A.

doi:10.1016/j.mycres.2006.10.003

Cited by 29 publications

(17 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Rhizopus: Single-colony isolates were inoculated to MEA for analysis of morphology and temperature-dependent growth per procedures, keys and descriptions in Liou et al (2007), Schipper and Stalpers (1984) and Schipper (1984).…”

Section: Fusariummentioning

confidence: 99%

Mycobiota of <em>Lupinus albus</em> seeds from a public germplasm collection

Alomran¹,

Lupien²,

Dugan³

2013

North American Fungi

View full text Add to dashboard Cite

Seedborne mycobiota of Lupinus albus was assessed using blotter paper and agar media with Rose Bengal or semi-selective media for Pythium or Fusarium. Samples of 200 seeds were taken from each of 16 inventories, comprising 14 accessions originating from Germany, France, Ukraine, Syria, Hungary or Spain, and increased 1-2 times through propagation by the USDA-ARS Western Regional Plant Introduction Station. A total of 307 fungus isolates were recovered and preserved with over 96% belonging to 10 genera: Penicillium (47%), Cladosporium (32%, including Cladosporium-like Chalastospora), Alternaria (4%), Aspergillus (4%), Fusarium (3%), Rhizopus (3%), Ulocladium (3%), Chaetomium (0.5%) and Arthrinium (< 0.5%). By sub-sampling, fungi were further identified by morpho-cultural characters and/or DNA sequences as Alternaria infectoria, A. alternata, and A. tenuissima species-groups, Arthrinium arundinus, Aspergillus niger, Chalastospora gossypii, Cladosporium herbarum and C. cladosporioides species complexes, Chaetomium sp., Fusarium Fungi 8(4): 1-15 acuminatum, Rhizopus stolonifer, and Ulocladium atrum group. Penicillia included P. hordei, but most penicillia could not be assigned species names either by morphological traits or β-tubulin DNA sequence data.

show abstract

Section: Fusariummentioning

confidence: 99%

Mycobiota of <em>Lupinus albus</em> seeds from a public germplasm collection

Alomran¹,

Lupien²,

Dugan³

2013

North American Fungi

View full text Add to dashboard Cite

show abstract

“…However, R. rhizopodiformis has the necessary apomorphy to be classified close to other Rhizopus. Liou et al (2007) had similar results when they showed linages of R. rhizopodiformis among the main glucoamylase-producing species, what demonstrates the need for more information on this species.…”

Section: Phylogenetic Analysismentioning

confidence: 58%

Bioprospection and characterization of the amylolytic activity by filamentous fungi from Brazilian Atlantic Forest

et al. 2017

View full text Add to dashboard Cite

Filamentous fungi are widely diverse and ubiquitous organisms. Such biodiversity is barely known, making room for a great potential still to be discovered, especially in tropical environments -which are favorable to growth and species variety. Filamentous fungi are extensively applied to the production of industrial enzymes, such as the amylases. This class of enzymes acts in the hydrolysis of starch to glucose or maltooligosaccharides. In this work twenty-five filamentous fungi were isolated from samples of decomposing material collected in the Brazilian Atlantic Forest. The two best amylase producers were identified as Aspergillus brasiliensis and Rhizopus oryzae. Both are mesophilic, they grow well in organic nitrogen-rich media produce great amounts of glucoamylases. The enzymes of A. brasiliensis and R. oryzae are different, possibly because of their phylogenetical distance. The best amylase production of A. brasiliensis occurred during 120 hours with initial pH of 7.5; it had a better activity in the pH range of 3.5-5.0 and at 60-75°C. Both fungal glucoamylase had wide pH stability (3-8) and were activated by Mn 2+. R. oryzae best production occurred in 96 hours and at pH 6.5. Its amylases had a greater activity in the pH range of 4.0-5.5 and temperature at 50-65ºC. The most significant difference between the enzymes produced by both fungi is the resistance to thermal denaturation: A. brasiliensis glucoamylase had a T 50 of 60 minutes at 70ºC. The R. oryzae glucoamylase only had a residual activity when incubated at 50°C with a 12 min T 50 . Keywords: Amylase, Filamentous fungi, Aspergillus brasiliensis, Rhizopus oryzae, Glucoamylase, Bioprospection Bioprospecção e caracterização da atividade amilolítica de fungos filamentosos da Mata Atlântica BrasileiraResumo: Fungos filamentosos são organismos amplamente diversificados e ubíquos. Esta biodiversidade ainda é pouco caracterizada, desta forma, há um grande potencial a ser descoberto, sobretudo em biomas tropicais, que favorecem o crescimento e diversificação de espécies. Fungos filamentosos são extensivamente utilizados para a produção industrial de enzimas, como as amilases. Esta classe de enzimas atua na hidrólise do amido em glicose ou maltooligossacarídeos. Neste trabalho 25 cepas de fungos filamentosos foram isoladas a partir de amostras de material em decomposição coletados na Mata Atlântica Brasileira. As duas cepas que produziram mais amilases foram identificadas como Aspergillus brasiliensis e Rhizopus oryzae. Ambos os fungos são mesofílicos, crescem bem em meio de cultivo rico em nitrogênio orgânico, e produziram grande quantidade de glucoamilase. As enzimas de A. brasiliensis e R. oryzae possuem características distintas, possivelmente devido à distância filogenética das espécies. A produção de amilase mais expressiva de A. brasiliensis ocorreu em 120 horas de cultivo e pH inicial de 7,5; possui maior atividade em temperaturas entre 60-75ºC e pH entre 3,5-5,0. Ambas glucoamilases fúngicas obtiveram ampla estabilidade de pH (3-8) e fora...

show abstract

“…Given the limitation of phenotypic identification methods, rDNA-based gene sequences have been used extensively for the molecular identification of fungi, including zygomycetes (1,11,23). The rDNA comprises a small-subunit gene (18S), a large-subunit gene (28S), and internal transcribed spacer (ITS) regions (ITS1 and ITS2).…”

Section: Case Reportmentioning

confidence: 99%

Cavitary Pulmonary Zygomycosis Caused by Rhizopus homothallicus

Chakrabarti¹,

Marak

Shivaprakash³

et al. 2010

J Clin Microbiol

View full text Add to dashboard Cite

We report the first two proven cases of cavitary pulmonary zygomycosis caused by Rhizopus homothallicus. The diagnosis in each case was based on histology, culture of the causal agent, and the nucleotide sequence of the D1/D2 region of the 28S ribosomal DNA. CASE REPORTCase 1 involved a 47-year-old male with a history of type II diabetes mellitus for 13 years who developed diabetic nephropathy leading to end-stage renal disease. He had undergone renal transplantation and was on triple-drug immunosuppression (cyclosporine, azathioprine, and prednisolone). One month posttransplantation, he developed an intermittent fever with chills and pleuritic chest pain. A chest X-ray revealed a cavitary lesion in the right upper lobe with bilateral nodular infiltrates, for which he was treated with a primary drug regimen of antituberculosis therapy (ATT), although multiple sputum examinations were negative for acid-fast bacilli. The patient did not respond to the ATT and was referred to our institute (Postgraduate Institute of Medical Education and Research [PGIMER], Chandigarh, India). At our institute, contrast-enhanced computed tomography (CT) of the thorax revealed a thick, smooth-walled cavitary lesion of the posterior segment of the right upper lobe and multiple nodules in both lungs (Fig. 1). No pleural effusion was noticed. Echocardiography ruled out endocarditis. His blood sugar levels ranged from 153 to 226 mg/dl. While he was at the hospital, he was treated with human insulin therapy. ATT was continued along with vancomycin and tazobactam. Ultrasound-guided fine-needle aspiration of the cavitary lesion of the lung was performed. Direct microscopic examination of calcofluor white-stained mounts of the aspirated fluid was done. Part of the fluid was cultured on Sabouraud dextrose agar (SDA) and brain heart infusion agar (HiMedia, Mumbai, India). Direct microscopy of the aspirated fluid did not reveal any fungal elements, nor did the cultures yield any fungal colonies during 4 weeks of incubation. As the patient did not improve until the 20th day of his hospital stay, an open-lung biopsy was performed. It revealed pus in the pleural cavity with a large cavitary lesion in the right upper lobe. No apparent mass was noticed. A wedge-shaped biopsy sample was taken from the site of the lesion. Tissue slides stained by the hematoxylin-and-eosin and periodic acid-Schiff procedures showed dense acute inflammatory infiltrates across the interstitial septa, a dense fibrosis surrounding the alveoli, extensive necrosis with nuclear debris and broad, aseptate, ribbon-like hyphae (Fig. 2). The causal fungus was observed invading the vessel wall. Culture of the biopsy tissue on SDA (HiMedia, Mumbai, India) grew a fast-growing, floccose white colony turning grayish. Microscopic examination of the colony showed broad, aseptate hyphae with lateral not-well-developed sporangiophores bearing globose sporangia containing a small number of sporangiospores. In addition, a large number of golden brown zygospores with stellate walls and wit...

show abstract

Polyphasic approach to the taxonomy of the Rhizopus stolonifer group

Cited by 29 publications

References 22 publications

Mycobiota of <em>Lupinus albus</em> seeds from a public germplasm collection

Mycobiota of <em>Lupinus albus</em> seeds from a public germplasm collection

Bioprospection and characterization of the amylolytic activity by filamentous fungi from Brazilian Atlantic Forest

Cavitary Pulmonary Zygomycosis Caused by Rhizopus homothallicus

Contact Info

Product

Resources

About