Polyphasic characterization of and genomic insights into a haloalkali-tolerant Saccharibacillus alkalitolerans sp. nov., that produces three cellulase isozymes and several antimicrobial compounds

Darji, Himanshu; Verma, Neha; Lugani, Yogita; Mehrotra, Priyam; Sindhu, Dev Kant; Vemuluri, Venkata Ramana

doi:10.1007/s10482-021-01575-x

Cited by 2 publications

(11 citation statements)

References 28 publications

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“…Interestingly, cellulase activity is strain-dependent, with S. sacchari and S. alkalitolerans showing signi cant cellulase isozyme activity (Rivas et al, 2008, Darji et al, 2021, while S. kuerlensis showed no cellulase activity (Yang et al, 2009). Our data agree with these previously published ndings.…”

Section: Discussionsupporting

confidence: 92%

“…Neither strain ATSA2 T nor the Saccharibacillus genus show any amylase activity (Rivas et al, 2008, Darji et al, 2021. Furthermore, the ATSA2 T genome only harbored a glgA gene (Supplementary Table S1), thus potentially providing an explanation for the apparent absence of amylase activity.…”

Section: Amylasementioning

confidence: 99%

“…There are seven published species of the genus Saccharibacillus; however, limited secondary metabolites have been studied, and the potential source of natural products in this genus remains untapped. Bacteriocin, thiopeptide, terpene, and nonribosomal peptide (NRP) synthase clusters have been identi ed in the S. alkalitolerans VR-M41 T genome(Darji et al, 2021). Among these compounds, some bacteriocin is produced by plant growth-promoting bacteria and can promote plant growth(Gray et al, 2006, Lee et al, 2009.…”

mentioning

confidence: 99%

“…Plant-associated bacteria, especially plant endophytes, play an important role in PGP and productivity (Hardoim et al, 2008, Santoyo et al, 2016, Chen et al, 2019, Singh et al, 2021).To the best of our knowledge, there have been no studies on interactions between bacteria of the genus Saccharibacillus and plants. The genus Saccharibacillus has been proven to have strong cellulase activity(Rivas et al, 2008, Darji et al, 2021. Cellulase, a cell wall-degrading enzyme, can affect the structural integrity of the host plants, thereby indirectly promoting host plant growth, suggesting a role for bacteria of the genus Saccharibacillus in plant growth and/or biocontrol.…”

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confidence: 99%

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Genome Insights into the Plant Growth-Promoting bacterium Saccharibacillus brassicae ATSA2T

Jiang

Seo

Peng

et al. 2022

Preprint

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Endophytes can facilitate the improvement of plant growth and health in agriculturally important crops, yet their genomes and secondary metabolites remain largely unexplored. We previously isolated Saccharibacillus brassicae strain ATSA2T from surface-sterilized seeds of kimchi cabbage and represented a novel species of the genus Saccharibacillus. In this study, we evaluated the plant growth-promoting effect of strain ATSA2T in kimchi cabbage, bok choy, and pepper plants grown in soils. We found a significant effect on the shoot and root biomass and chlorophyll contents following strain ATSA2T treatment. Furthermore, genome analysis of this strain suggested the presence of gene clusters involved in iron acquisition (fhuABD, afuABC, fbpABC, and fepCDG) and phosphate solubilization (pstABCHS, phoABHLU, and phnCDEP) and other phytohormone biosynthesis genes, including indole-3-acetic acid (trpABCDEFG), in the genome. Interestingly, the secondary metabolites cerecidin, carotenoid, siderophore (staphylobactin), and bacillaene underlying plant growth promotion were found in the whole genome via antiSMASH analysis. Overall, physiological testing and genome analysis data provide comprehensive insights into plant growth-promoting mechanisms, suggesting the relevance of strain ATSA2T in agricultural biotechnology.

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Section: Discussionsupporting

confidence: 92%

Section: Amylasementioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Genome Insights into the Plant Growth-Promoting bacterium Saccharibacillus brassicae ATSA2T

Jiang

Seo

Peng

et al. 2022

Preprint

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“…Saccharibacillus is a genus of gram-positive, endospore-forming, facultatively anaerobic rod-shaped bacteria, of which there are seven published species, with the major species having been isolated from plants (Jiang et al 2020 ; Darji et al 2021 ; Kampfer et al 2016 ; Rivas et al 2008 ). To date, there have been no functional studies in plants with bacteria of this genus, and few secondary metabolites have been studied.…”

Section: Introductionmentioning

confidence: 99%

Genome insights into the plant growth-promoting bacterium Saccharibacillus brassicae ATSA2T

et al. 2023

View full text Add to dashboard Cite

Endophytes can facilitate the improvement of plant growth and health in agriculturally important crops, yet their genomes and secondary metabolites remain largely unexplored. We previously isolated Saccharibacillus brassicae strain ATSA2T from surface-sterilized seeds of kimchi cabbage and represented a novel species of the genus Saccharibacillus. In this study, we evaluated the plant growth-promoting (PGP) effect of strain ATSA2T in kimchi cabbage, bok choy, and pepper plants grown in soils. We found a significant effect on the shoot and root biomass, and chlorophyll contents following strain ATSA2T treatment. Strain ATSA2T displayed PGP traits such as indole acetic acid (IAA, 62.9 μg/mL) and siderophore production, and phosphate solubilization activity. Furthermore, genome analysis of this strain suggested the presence of gene clusters involved in iron acquisition (fhuABD, afuABC, fbpABC, and fepCDG) and phosphate solubilization (pstABCHS, phoABHLU, and phnCDEP) and other phytohormone biosynthesis genes, including indole-3-acetic acid (trpABCDEFG), in the genome. Interestingly, the secondary metabolites cerecidin, carotenoid, siderophore (staphylobactin), and bacillaene underlying plant growth promotion were found in the whole genome via antiSMASH analysis. Overall, physiological testing and genome analysis data provide comprehensive insights into plant growth-promoting mechanisms, suggesting the relevance of strain ATSA2T in agricultural biotechnology.

show abstract

Polyphasic characterization of and genomic insights into a haloalkali-tolerant Saccharibacillus alkalitolerans sp. nov., that produces three cellulase isozymes and several antimicrobial compounds

Cited by 2 publications

References 28 publications

Genome Insights into the Plant Growth-Promoting bacterium Saccharibacillus brassicae ATSA2T

Genome Insights into the Plant Growth-Promoting bacterium Saccharibacillus brassicae ATSA2T

Genome insights into the plant growth-promoting bacterium Saccharibacillus brassicae ATSA2T

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