Polyphasic identification of a thermophilic bacterium from geyser of Cisolok, Indonesia

Mawarid, Abinubli Tariswafi; Sjamsuridzal, Wellyzar; Yabe, Shuhei; Santoso, Iman; Ningsih, Fitria; Nurlaili, Dafina Ghossani; Oetari, Ariyanti; Yokota, Akira

doi:10.1063/1.4946970

Cited by 2 publications

(4 citation statements)

References 11 publications

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“…According to Lee et al (2018), Actinobacteria was one of the classes that contributed to almost 69% of the total population in the degraded foliage microbiota from foliage-rich hot spring in Sungai Klah, Malaysia, based on metagenomic analysis. Our previous studies, Mawarid et al (2016) and Yokota et al (2016), have reported the xylan-degrading ability of thermophilic bacteria from the phylum Firmicutes isolated from litter in Cisolok geyser, e.g. Brevibacillus sp.…”

Section: Screening Of Xylan-degrading Abilitymentioning

confidence: 99%

“…Bacterial communities in geothermal areas in Indonesia are still rarely explored, although these communities are potential producers of thermostable enzymes. Cisolok geothermal area in West Java, Indonesia is one of potential habitat from where thermophilic bacteria can be isolated (Mawarid et al 2016;Yokota et al 2016;Ningsih et al 2019). Yokota et al (2016) have proposed a new species of thermophilic bacterium, namely Paenibacillus cisolokensis, isolated from litter of Cisolok geysers, which can hydrolyze various substrates, such as starch, xylan, and cellulose at temperature of 50°C.…”

Section: Introductionmentioning

confidence: 99%

“…Another thermophilic bacterium, Brevibacillus sp. UICC B-76, isolated from litter of Cisolok geysers, demonstrated xylanase, amylase, and cellulase activities at temperature of 50°C (Mawarid et al 2016). Thus, the Cisolok geothermal area is a promising source of thermophilic enzyme producers.…”

Section: Introductionmentioning

confidence: 99%

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Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

Rachmania¹,

Ningsih²,

Setyaningsih³

et al. 2019

Biodiversitas

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Abstract. Rachmania MK, Ningsih F, Setyaningsih PP, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridjal W. 2020. Xylan-degrading ability of thermophilic Actinobacteria isolated from soil in a geothermal area. Biodiversitas 21: 144-154. This study was conducted to obtain the potential isolates of xylan-degrading thermophilic Actinobacteria. Seventeen isolates were obtained from the soil samples collected from Cisolok geysers in West Java, Indonesia. These isolates were screened for their xylan-degrading ability on minimal medium with the addition of 0.5% xylan as substrate, and were incubated at various temperatures for 7 days. A total of 15, 14, 4, and 3 isolates showed a xylan-degrading ability at 45°C, 50°C, 55°C, and 60°C, respectively. The three isolates (SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4) that showed xylan-degrading ability at 60°C on 0.5% xylan were further tested by using minimal medium with the addition of 0.1% RBB-xylan as a substrate. The results showed that these isolates were also able to utilized RBB-xylan at 45 to 60°C. The xylanolytic activity of the crude enzymes from the three isolates on minimal medium containing 0.5% xylan or 0.1% RBB-xylan as substrates, indicated that these isolates were able to produce extracellular xylanase. This study revealed that the soil of Cisolok geysers served as an ideal source for finding the thermophilic Actinobacteria which produced xylanase at high temperatures.

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Section: Screening Of Xylan-degrading Abilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

Rachmania¹,

Ningsih²,

Setyaningsih³

et al. 2019

Biodiversitas

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“…The Cisolok geothermal area, as one of less-explored extreme environments in West Java, is one of the potential habitats which has high microbial diversity with Proteobacteria and Cyanobacteria as major abundant phyla (Seo et al 2012). Previous studies by Yokota et al (2016) and Mawarid et al (2016) reported that thermophilic bacteria identified as Paenibacillus cisolokensis and Brevibacillus sp., respectively, isolated from the litter of Cisolok geothermal area, which showed positive results for cellulase enzyme activity at temperature of 50°C.…”

Section: Introductionmentioning

confidence: 99%

Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia

et al. 2019

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Abstract. Setyaningsih PP, Ningsih F, Rachmania MK, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridzal W. 2019. Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia. Biodiversitas 20: 3134-3141. This study investigated 17 thermophilic Actinobacteria isolated from the soil of geysers in the Cisolok geothermal area, West Java, as potential producers of cellulase. Screening for cellulase was performed on minimal (Mm) agar medium with and without the addition of 1% (w/v) carboxymethylcellulose (CMC) and microcrystalline cellulose (MCC), then incubated at 45, 50, 55 and 60°C for up to 7 days. Formation of clear zones around colonies indicated cellulose hydrolysis. The results showed that 15, 14, 4, and 3 isolates showed cellulolytic activity on CMC agar medium at 45, 50, 55, and 60°C, respectively, after 7 days of incubation. Three potential isolates showed cellulolytic activity on MCC agar medium after being incubated for 7 days at 45°C. Molecular identification based on the 16S rRNA gene was performed for three isolates with positive cellulolytic activity at 60°C. The results showed that the three isolates are closely related to Actinomadura keratinilytica WCC-2265T with 99.93-100% sequence similarities. A phylogenetic tree based on 16S rRNA gene sequences confirmed that the three isolates were clustered together with Actinomadura keratinilytica WCC-2265T with 100% bootstrap value. The tree also showed that cellulase producers and non-cellulase producers in Thermomonosporaceae are grouped into different clades.

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Polyphasic identification of a thermophilic bacterium from geyser of Cisolok, Indonesia

Cited by 2 publications

References 11 publications

Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia

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