Polyphenol associated-DNA adducts in lung and blood mononuclear cells from lung cancer patients

Várkonyi, Andrea; Kelsey, Karl T.; Semey, Karen; Bodell, William J.; Lévay, György; Mark, Eugen; Wain, John C.; Christiani, David C.; Wiencke, John K.

doi:10.1016/j.canlet.2005.04.034

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…Therefore, it is not clear whether smoking increases DSBs in the cells of arterial wall from this study. However, some studies have described a correlation between the numbers of DNA adduct in leukocytes and lungs in smokers [29]. We used DNA damage in MNCs as a surrogate marker for that in vascular cells, because MNCs are exposed to the bloodstream in a manner similar to that of vascular cells.…”

Section: Discussionmentioning

confidence: 99%

Smoking Cessation Reverses DNA Double-Strand Breaks in Human Mononuclear Cells

Ishida

Tashiro

et al. 2014

PLoS ONE

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ObjectiveCigarette smoking is a major risk factor for atherosclerotic cardiovascular disease, which is responsible for a significant proportion of smoking-related deaths. However, the precise mechanism whereby smoking induces this pathology has not been fully delineated. Based on observation of DNA double-strand breaks (DSBs), the most harmful type of DNA damage, in atherosclerotic lesions, we hypothesized that there is a direct association between smoking and DSBs. The goal of this study was to investigate whether smoking induces DSBs and smoking cessation reverses DSBs in vivo through examination of peripheral mononuclear cells (MNCs).Approach and ResultsImmunoreactivity of oxidative modification of DNA and DSBs were increased in human atherosclerotic lesions but not in the adjacent normal area. DSBs in human MNCs isolated from the blood of volunteers can be detected as cytologically visible “foci” using an antibody against the phosphorylated form of the histone H2AX (γ-H2AX). Young healthy active smokers (n = 15) showed increased γ-H2AX foci number when compared with non-smokers (n = 12) (foci number/cell: median, 0.37/cell; interquartile range [IQR], 0.31–0.58 vs. 4.36/cell; IQR, 3.09–7.39, p<0.0001). Smoking cessation for 1 month reduced the γ-H2AX foci number (median, 4.44/cell; IQR, 4.36–5.24 to 0.28/cell; IQR, 0.12–0.53, p<0.05). A positive correlation was noted between γ-H2AX foci number and exhaled carbon monoxide levels (r = 0.75, p<0.01).ConclusionsSmoking induces DSBs in human MNCs in vivo, and importantly, smoking cessation for 1 month resulted in a decrease in DSBs to a level comparable to that seen in non-smokers. These data reinforce the notion that the cigarette smoking induces DSBs and highlight the importance of smoking cessation.

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Section: Discussionmentioning

confidence: 99%

Smoking Cessation Reverses DNA Double-Strand Breaks in Human Mononuclear Cells

Ishida

Tashiro

et al. 2014

PLoS ONE

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“…65 Adduct levels are also consistently higher in lung tissue of smokers compared with nonsmokers. 32,54,66 Varkonyi et al 67 reported a correlation between adduct levels in blood mononuclear cells and lung tissue from lung cancer patients, and they also reported the detection of adducts chromatographically similar to those derived from hydroquinone and benzenetriol, metabolites of benzene. Gyorffy et al 68 compared DNA adducts in normal lung and tumor tissue by both Abbreviations: GC: gas chromatography; LC: liquid chromatography; HPLC: high-performance liquid chromatography; MS: mass spectrometry; ESI: electrospray ionization; NSI-HRMS: nanoelectrospray high-resolution mass spectrometry; NICI: negative ion chemical ionization; MALDI: matrixassisted laser desorption ionization; TOF: time of flight; SRM: selection reaction monitoring; ELISA: enzyme-linked immunosorbent assay.…”

Section: Detection Methods For Smoking-induced Dna and Protein Damagementioning

confidence: 98%

“…Adduct levels are also consistently higher in lung tissue of smokers compared with nonsmokers 32, 54, 66. Varkonyi et al 67. reported a correlation between adduct levels in blood mononuclear cells and lung tissue from lung cancer patients, and they also reported the detection of adducts chromatographically similar to those derived from hydroquinone and benzenetriol, metabolites of benzene.…”

Section: Smoking‐related Dna Adducts In Human Tissuesmentioning

confidence: 96%

DNA and protein adducts in human tissues resulting from exposure to tobacco smoke

Phillips

Venitt

2012

Intl Journal of Cancer

125

122

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Tobacco smoke contains a variety of genotoxic carcinogens that form adducts with DNA and protein in the tissues of smokers. Not only are these biochemical events relevant to the carcinogenic process, but the detection of adducts provides a means of monitoring exposure to tobacco smoke. Characterization of smoking‐related adducts has shed light on the mechanisms of smoking‐related diseases and many different types of smoking‐derived DNA and protein adducts have been identified. Such approaches also reveal the potential harm of environmental tobacco smoke (ETS) to nonsmokers, infants and children. Because the majority of tobacco‐smoke carcinogens are not exclusive to this source of exposure, studies comparing smokers and nonsmokers may be confounded by other environmental sources. Nevertheless, certain DNA and protein adducts have been validated as biomarkers of exposure to tobacco smoke, with continuing applications in the study of ETS exposures, cancer prevention and tobacco product legislation. Our article is a review of the literature on smoking‐related adducts in human tissues published since 2002.

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“…Outro ponto somatório e agravante da exposição a HQ é a exposição ambiental, visto que este agente fenólico esta presente na composição de uma gama variada de alimentos e também presente no ar de grandes centros urbanos na forma de BZ, precursor da HQ (DECAPRIO, 1999;DUARTE et al, 1999;KHALEQUZZAMAN et al, 2010;WHITWORTH et al, 2011). A união destas fontes de exposição pode traduzir-se em uma cronicidade da exposição, embora sejam a baixas concentrações e a literatura apresenta vários efeitos deletérios aos sistemas biológicos, como câncer e problemas respiratórios, advindos da exposição a este agente fenólico (JIANG, G. F. et al, 2003;VARKONYI et al, 2006;JIANG, G. et al, 2008;MONKS et al, 2011) Como descrito anteriormente, um efeito bem caracterizado da exposição a HQ é o estresse oxidativo, pois a HQ ao ser metabolizado conjuga-se com a glutationa o que pode levar a HQ a entrar no ciclo redox, o que eleva a produção de…”

Section: Efeitos Deletérios Da Exposição Ocupacional E Ambiental à Hqunclassified

Caracterização dos efeitos da exposição à hidroquinona sobre o recrutamento leucocitário para o pulmão inflamado

Ribeiro¹

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Polyphenol associated-DNA adducts in lung and blood mononuclear cells from lung cancer patients

Cited by 6 publications

References 12 publications

Smoking Cessation Reverses DNA Double-Strand Breaks in Human Mononuclear Cells

Smoking Cessation Reverses DNA Double-Strand Breaks in Human Mononuclear Cells

DNA and protein adducts in human tissues resulting from exposure to tobacco smoke

Caracterização dos efeitos da exposição à hidroquinona sobre o recrutamento leucocitário para o pulmão inflamado

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