Polyphenol characterisation and diverse bioactivities of native Australian lilly pilly (Syzygium paniculatum) extract

Abstract

Magenta lilly pilly (Syzygium paniculatum) is an Australian native tree that produces berry fruits that are rich in phytochemicals reportedly beneficial to human health. Here we explored the biological activities...

Structural Analysis and Identity Confirmation of Anthocyanins in Brassica oleracea Extracts by Direct Injection Ion Mobility-Mass Spectrometry

“…Red cabbage, purple kale, purple Brussels sprouts, and purple cauliflower (500 g each) were purchased from local supermarkets in Adelaide, Australia, in 2021. Samples of red cabbage, purple kale, purple Brussels sprouts and purple cauliflower were prepared as outlined previously . Briefly, the lyophilized plant material was extracted twice at room temperature with 2% aqueous formic acid (FA), with the supernatants collected and combined after centrifugation.…”

“…Samples of red cabbage, purple kale, purple Brussels sprouts and purple cauliflower were prepared as outlined previously. 11 Briefly, the lyophilized plant material was extracted twice at room temperature with 2% aqueous formic acid (FA), with the supernatants collected and combined after centrifugation. The supernatants were then loaded onto an Amberlite XAD7HP resin (Sigma Aldrich, St. Louis, USA) and washed with 2% aqueous FA to remove unbound compounds and then with 2% FA in methanol to elute the remaining anthocyanins.…”

“…Red cabbage extracts were dissolved in 2% aqueous FA (1 mg/mL) and analyzed using an Agilent 1260 Infinity LC coupled to an Agilent 1290 Infinity II Diode Array Detector and an Agilent 6495B Triple Quadrupole in a HPLC-DAD-QQQ platform (Agilent Technologies, Santa Clara, USA) as described previously. 11 Briefly, red cabbage extract (5 μL) was injected and passed through an Agilent Poroshell 120 SB-C18 column (100 mm × 2.1 mm × 2.7 μm) (Agilent Technologies, Santa Clara, USA) at a flow rate of 0.3 mL min −1 . DAD detection was set at 520 nm.…”

“…Analysis of anthocyanins in plant extracts typically involves coupling liquid chromatography to diode array detection (LC-DAD) and mass spectrometry (LC-DAD-MS), whereby optical absorbance is used for classification and quantification of individual anthocyanin species as identified by MS . These approaches provide simultaneous retention time and spectroscopic information for all observable species present, and, for this reason, have been used to elucidate the anthocyanin composition of countless plant extracts. − However, such analyses often rely on sample-dependent LC optimization, which can drastically increase sample run times, leading to an overall reduction in the throughput of analysis. LC-DAD-MS approaches also yield limited structural information and hence, when profiling complex extracts that contain classes of compounds with similar structural features, such approaches are unable to elucidate clear structural trends.…”

Structure-dependent detection of polyphenols using crown ether-immobilized gold nanoparticles

“…Polyphenol molecules contain aromatic rings, therefore they are detectable by High Performance Liquid Chromatography (HPLC) with an UV detector. For example, polyphenols contained in beverage, plant and biological samples have been separated and analyzed using HPLC or LC-MS. [11][12][13][14][15][16] Recently, quantitative nuclear magnetic resonance spectroscopy was applied to polyphenol detection. 17 Many polyphenol molecules have a phenol, 1,2-,1,3-dihydroxylbenzene (catechol or resorcinol) or 1,2,3-trihydroxylbenzene (pyrogallol) structure.…”