Polyphenol Contents, Antioxidant and Antibacterial Activities of Aqueous Algerian Propolis Extracts

Abstract

The propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties, mainly attributed to the presence of polyphenols. The composition of propolis depends on time, vegetation, and the area of collection. Total flavonoid and polyphenol contents of aqueous extracts of propolis samples from different areas of Algeria, determined by using aluminum chloride and Folin–Ciocalteu colorimetric methods, were in the range of 3.047 ± 0.004–5.273 ± 0.013 mg/g and 96.833 ± 0.027–458.833 ± 0.0005 mg/g crude extract of propolis, respectively. This study examined the antioxidant and antimicrobial activities of propolis. Aqueous extracts of propolis were obtained in order to evaluate their antioxidant activities by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, β-carotene and electrochemical assays. All tested propolis samples had relatively strong antioxidant activities, which were also correlated with the total polyphenol and flavonoid content present. The percentage of inhibition of lipid peroxidation of linoleic acid emulsion during 24 h varied between 86.04 ± 0.42 and 90.60 ± 3.77% among the tested samples. The highest DPPH radical scavenging activity was observed by ABAL (Ain Abassa Aqueous Extract) with IC50 = 8.49 ± 5.07 10−5 μg/ml, and the lowest was observed by SAL (Setif Aqueous Extract) with IC50 of 21.16 ± 0.0001 μg/ml. The most important antibacterial activity was obtained with Ain Abassa extract; the zones of inhibition obtained for this excerpt vary from 15.22 to 15.5 mm. Followed by the Setif extract with areas of 12.33 to 12.75 mm, the Tizi-Ouzou extract with areas of 10.11 to 11.11 mm. This study will bring an innovation for further studies with regard to the antioxidant and antibacterial properties of the aqueous extracts of propolis. This study corroborates that Algerian propolis is a rich source of natural antioxidants, properties which could be used in the prevention of different diseases, both in humans and in animals.

Bioactive Compounds, Antioxidant and Antimicrobial Activity of Propolis Extracts during In Vitro Digestion

“…In addition, these values are lower than ours [29]. On the other hand, aqueous extracts of propolis from Algeria have a total phenol content of 9688.3 to 45,883.3 mg EGA/100 g, the variation was mainly due to the place of origin of the propolis [30]. On the other hand, there are various methods for the purification of extracts, which can influence the content of bioactive compounds.…”

“…500 mg EQ/100 g). Various investigations report a total flavonoid content of 3823 to 4745 mg EQ/100 g [31]; 304.7 to 527.3 mg EQ/100 g [30]; 16,622 to 51,977 mg EQ/100 g [27]; 1100 to 15,000 mg EQ/100 g [7]; 4946.5 mg EQ/100 g [33]; and 55,758 mg EQ/100 g [26]. As we can see, the flavonoids' content is highly variable; therefore, biological activity could vary.…”

Antiviral and Anti-Quorum Sensing Activties of Lyophilized Aqueous Extract of Propolis From Sétif

“…Pretreatment and continuous treatment of propolis extract in NIH/3T3 cell cultures showed an impressive inhibition of malignant cell transformation by Moloney murine Sarcoma Virus (MuSV) (Huleihel and Ishano, 2001). The antiviral and the anti proliferative activities of the extract of propolis could be attributed to its contents in flavonoids (3,047 ± 0,004 mg/g), polyphenols (221 ± 0,001 mg/g), and its four identified derivatives of cinnamic acid (Soltani, 2017;Soltani et al, 2021). Cinnamaldehyde a derivative of cinnamic acid has been reported as an inducer of apoptosis on various cancer cell lines in vitro, that transduced the apoptotic signal via reactive oxygen species (ROS) generation (Ka et al, 2003;Li et al, 2016) In the presence of Vibrio harveyi BB120, with and without the addition of the extract Artemia survives differently.…”