Polyphenol gene expression and changes in anthocyanins and polyphenols in the skin of ‘Braeburn’ apples after the autumn application of prohexadione-calcium

Bizjak, Jan; Weber, Nika; Mikulič-Petkovšek, Maja; Alam, Zobayer; Thill, Jana; Stich, Karl; Halbwirth, Heidi; Veberič, Robert

doi:10.1007/s10725-013-9823-6

Cited by 10 publications

(13 citation statements)

References 16 publications

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“…Earlier, Halbwirth et al (2006) proposed that F3H and FLS, the two 2-ODDs genes, were inhibited in young grapevine leaves, flowers, and berries after Pro-Ca treatment. The inhibition of F3H had been proved in the skin of apples, while the expression levels of PAL, CHS, F3H, DFR, ANS and FGT were also reduced in varying degrees after treatment (Bizjak et al, 2013). In our study, we also noted that the expression of PlCHS, PlF3H, PlF3'H, PlDFR, PlANS and PlF3GT was lower levels in petals with Pro-Ca treatment.…”

Section: Discussionsupporting

confidence: 73%

“…Also, the color loss was directly related to a decrease in anthocyanin content (Schmitzer et al, 2012). Bizjak et al (2012Bizjak et al ( , 2013 found that the inhibition of Pro-Ca on enzyme activities of 2-ODDs flavanone 3-hydroxylase gene (F3H), flavonol synthase gene (FLS) and anthocyanidin synthase gene (ANS) in flavonoid biosynthesis pathway in the skin of apples might lead to a decrease in anthocyanin content. Up to now, few studies have focused on the effect of Pro-Ca treatment in ornamental plants, yet the detailed mechanism of how Pro-Ca would modify floral color is still not clear.…”

Section: Introductionmentioning

confidence: 99%

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Prohexadione-calcium Modified Color Characteristics and Flavonoid Biosynthetic Genes Expression in Herbaceous Peony (Paeonia lactiflora) Flowers

Tang¹,

Zhao²,

Hao³

et al. 2017

IJAB

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Prohexadione-calcium (Pro-Ca), originally developed as a plant growth retardant, has recently been discovered it could modify floral color in several ornamental plants like petunia and rose. However, the underlying mechanism of Pro-Ca on the regulation of floral color is still elusive. In this study, a purple-red herbaceous peony (Paeonia lactiflora Pall.) cultivar with control and Pro-Ca treatment was used as the material. Their color indices were measured, flavonoid compositions and contents as well as its biosynthetic genes expression were analyzed. After Pro-Ca treatment, a remarkable rise in the color parameter lightness (L * ) value along with an obvious fall in the color parameter red/green (a * ) value indicated that the loss of red color was occurred. In addition, total anthocyanin, anthoxanthin and flavonoid contents were all decreased significantly after Pro-Ca treatment, which was in accordance with the color indices. Furthermore, the expression patterns of most relative genes in flavonoid biosynthetic pathway expressed an overall decline after Pro-Ca treatment. Among the 2-oxoglutaric aciddependent dioxygenase genes involved in the pathway, flavanone 3-hydroxylase gene (F3H) and anthocyanidin synthase gene (ANS) had the highest levels of expression, suggesting that the loss of red color might be the reason of the synergistic inhibitory effect of these enzymes especially F3H and ANS in response to Pro-Ca. These results would contribute to a theoretical foundation for the regulation of floral color in field P. lactiflora by Pro-Ca treatment.

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Section: Discussionsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Prohexadione-calcium Modified Color Characteristics and Flavonoid Biosynthetic Genes Expression in Herbaceous Peony (Paeonia lactiflora) Flowers

Tang¹,

Zhao²,

Hao³

et al. 2017

IJAB

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“…These phytochemicals in barley grains could be extracted as natural antioxidants. In fact, antioxidants have distinct effects on malting and brewing processes, including foam stability and beer bitterness, and flavonoids may affect beer taste, color and haze formation [ 5 – 8 ].…”

Section: Introductionmentioning

confidence: 99%

Association mapping for total polyphenol content, total flavonoid content and antioxidant activity in barley

et al. 2018

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BackgroundThe interest has been increasing on the phenolic compounds in plants because of their nutritive function as food and the roles regulating plant growth. However, their underlying genetic mechanism in barley is still not clear.ResultsA genome-wide association study (GWAS) was conducted for total phenolic content (TPC), total flavonoid content (FLC) and antioxidant activity (AOA) in 67 cultivated and 156 Tibetan wild barley genotypes. Most markers associated with phenolic content were different in cultivated and wild barleys. The markers bPb-0572 and bPb-4531 were identified as the major QTLs controlling phenolic compounds in Tibetan wild barley. Moreover, the marker bPb-4531 was co-located with the UDP- glycosyltransferase gene (HvUGT), which is a homolog to Arabidopsis UGTs and involved in biosynthesis of flavonoid glycosides .ConclusionsGWAS is an efficient tool for exploring the genetic architecture of phenolic compounds in the cultivated and Tibetan wild barleys. The DArT markers applied in this study can be used in barley breeding for developing new barley cultivars with higher phenolics content. The candidate gene (HvUGT) provides a potential route for deep understanding of the molecular mechanism of flavonoid synthesis.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4483-6) contains supplementary material, which is available to authorized users.

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“…For example, Kavalier et al (2014) showed that F3H was inhibited by Pro-Ca in the hop (Humulus lupulus L.) cones. In 'Braeburn' apple (Malus pumila), Bizjak et al (2013) found that the expression of F3H and ANS was reduced in response to autumn application of Pro-Ca. This reduction resulted in dramatic changes to the flavonoid composition in apple peels, associated with a loss of red coloration in the skin of the apple.…”

Section: Discussionmentioning

confidence: 99%

Daminozide reduces red color intensity in herbaceous peony (Paeonia lactiflora Pall.) flowers by inhibiting the expression of flavonoid biosynthetic genes

2018

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Daminozide is a plant growth retardant that inhibits 2-oxoglutarate-dependent dioxygenase activity in the gibberellin biosynthesis pathway, but few studies have focused on its effect on the flavonoid biosynthesis pathway involved in flower color. In the present study, the effect of daminozide on the regulation of flower color was investigated. An herbaceous peony ( Pall.) cultivar 'Fenzhu Pan' was used as the material and treated with daminozide. Color parameters of petals were measured; flavonoid composition and content as well as flavonoid biosynthetic gene expression were monitored. The results showed that daminozide treatment caused less red coloration in herbaceous peony. The content of total anthocyanins was decreased by 23%, and the content of total anthoxanthins was increased by 8%; thus, it appeared that less red coloration in daminozide-treated flowers was associated with restricted anthocyanin accumulation. Furthermore, the gene expression patterns of flavonoid 3'-hydroxylase, dihydroflavonol 4-reductase and anthocyanidin synthase were lower after daminozide treatment, which could decrease anthocyanin accumulation and lead to less red coloration in flowers. These findings would improve our understanding of daminozide regulation of flower color in herbaceous peony.

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Polyphenol gene expression and changes in anthocyanins and polyphenols in the skin of ‘Braeburn’ apples after the autumn application of prohexadione-calcium

Cited by 10 publications

References 16 publications

Prohexadione-calcium Modified Color Characteristics and Flavonoid Biosynthetic Genes Expression in Herbaceous Peony (Paeonia lactiflora) Flowers

Prohexadione-calcium Modified Color Characteristics and Flavonoid Biosynthetic Genes Expression in Herbaceous Peony (Paeonia lactiflora) Flowers

Association mapping for total polyphenol content, total flavonoid content and antioxidant activity in barley

Daminozide reduces red color intensity in herbaceous peony (Paeonia lactiflora Pall.) flowers by inhibiting the expression of flavonoid biosynthetic genes

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