2017
DOI: 10.1002/jobm.201700413
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Polyphenol oxidase fromPectobacterium atrosepticum: identification and cloning of gene and characteristics of the enzyme

Abstract: In the present study, we attempted to elucidate if the harmful phytopathogenic bacteria of Pectobacterium genus (P. atrosepticum) possess the enzymes for oxidation of phenolic compounds. Polyphenol oxidase (laccase) activity was revealed in P. atrosepticum cell lysates. Using bioinformatic analysis, an ORF encoding a putative copper-containing polyphenol oxidase of 241 amino acids with a predicted molecular mass of 25.9 kDa was found. This protein (named Pal1) shares significant level of identity with laccases… Show more

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Cited by 4 publications
(3 citation statements)
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“…Laccase assay Laccase (benzenediol: oxygen oxidoreductase; EC 1.10.3.2) enzymatic activities of recombinant FAMIN 254I , YfiH and YlmD were tested against the non-phenolic synthetic substrate ABTS [2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] as described (Gorshkov et al, 2017). 10 mg/mL of laccase from Trametes versicolor (positive control) or an equivalent quantity of purified FAMIN 254I , YfiH or YlmD was used in all assays.…”
Section: Enzyme Assaysmentioning
confidence: 99%
“…Laccase assay Laccase (benzenediol: oxygen oxidoreductase; EC 1.10.3.2) enzymatic activities of recombinant FAMIN 254I , YfiH and YlmD were tested against the non-phenolic synthetic substrate ABTS [2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] as described (Gorshkov et al, 2017). 10 mg/mL of laccase from Trametes versicolor (positive control) or an equivalent quantity of purified FAMIN 254I , YfiH or YlmD was used in all assays.…”
Section: Enzyme Assaysmentioning
confidence: 99%
“…Due to the presence of two Cu 2+ binding regions in the active center of PPO, Cu 2+ is considered an activator of PPO [83]. Testing of the effect of different Cu 2+ concentrations on the activity of Camellia sinensis PPO [78] showed that it had the highest catalytic activity when the Cu 2+ concentration was 10 −7 M. Although SDS, urea, and surfactants have been reported to activate some plant PPOs [5,84], they are considered to have no activating effect on Camellia sinensis PPO [69].…”
Section: Activators and Inhibitors Of Ppomentioning
confidence: 99%
“…Plant PPO proteins generally contain three conserved regions, namely an N-terminal cTP, a CuA and CuB (tyrosinase) domain, and a C-terminus extension ( Tran et al., 2012 ), which are responsible for thylakoid lumen localization and enzyme activity. PPO proteins are found in many species of terrestrial plants, such as apple ( Guardo et al., 2013 ), strawberry ( Jia et al., 2016 ), potato ( Chi et al., 2014 ), tomato ( Newman et al., 1993 ), banana ( Gooding et al., 2001 ), Populus ( He et al., 2021 ), rice (Yanchun Yu et al., 2008 ), wheat ( Beecher et al., 2012 ), barley ( Taketa et al., 2010 ), and eggplant ( Jukanti and Bhatt, 2015 ), as well as in fungi ( Li et al., 2011 ) and bacteria ( Gorshkov et al., 2017 ). However, no homologs of the PPO gene were found in the Arabidopsis thaliana genome ( Tran et al., 2012 ).…”
Section: Introductionmentioning
confidence: 99%