Polyphenolic constituents of the methanolic extract of Callistemon viridiflorous leaves and its antimicrobial activity

Abstract

Introduction: The genus Callistemon (family: Myrtaceae) contains 34 species which are widely distributed in the temperate regions and used in folk medicine. Methods: Chromatographic separation of 80% MeOH extract of the leaves of Callistemon viridiflorus (sims) Sweet (Cv) was performed. Results: Seven known polyphenolic compounds were isolated for the first time from this species: gallic acid, ellagic acid, isoquercetin, hyperin, 1,2:3,4-(bis(s)hexahydroxy diphenoyl-β-D-glucopyranose, nilocitin and quercetin-3-O-α-L-glucuronopyranoside. The methanolic extract of Cv leaves exhibited a significant anti-microbial activity against the tested microorganisms.

Chemical Constituents of the Ethyl Acetate Extract from Diaphragma juglandis Fructus and Their Inhibitory Activity on Nitric Oxide Production In Vitro

“…Compound 1 (peak 1 in Figure 2 A): ESI-MS ( m / z ): 169 [M − H] − , 171 [M + H] + , molecular formula C 7 H 6 O 5 , M = 170; 1 H-NMR (CD 3 OD- d 6 , 400 Hz) δ: 7.06 (2H, s, H-2, 6), 4.92 (-OH); 13 C-NMR (CD 3 OD- d 6 , 100 Hz) δ: 169.0 (C-7), 145.0 (C-3, 5), 138.2 (C-4), 120.6 (C-1), 108.9 (C-2, 6). Comparing the above data with the literature [ 17 , 18 ], compound 1 was identified as gallic acid.…”