Polyphenolic Metabolites from Iris pseudacorus

Polyphenolic Metabolites from Iris pseudacorus Roots

“…We reported earlier on the isolation of flavonoids and isoflavonoids from stems and leaves of unstressed I. pseudacorus L. [5]. The goals of the present research were to isolate polyphenols from roots of I. pseudacorus, to elucidate their structures, to determine the contents in roots and aerial parts (stems, leaves) of the plant, and to study their antitumor activity in vitro.Column chromatography over silica gel and Sephadex LH-20 isolated from CHCl 3 and hexane extracts of I. pseudacorus roots the isoflavonoids irilin B (8) [5], 5,7-dihydroxy-2c,6-dimethoxyisoflavone (11) [5], ayamenin A (16) [5], and the flavonoid 3,6-dimethoxy-5,7-dihydroxyflavone (14) [5]. Chromatography of the EtOH extract over Toyopearl HW-50 followed by purification over reversed-phase C-18 adsorbent isolated gallic (1) [6], 2,3,5-trihydroxybenzoic (2) [7], gentisic (3) [8], and p-hydroxybenzoic (4) acids [9] in addition to the new metabolite 5.…”

“…We reported earlier on the isolation of flavonoids and isoflavonoids from stems and leaves of unstressed I. pseudacorus L. [5]. The goals of the present research were to isolate polyphenols from roots of I. pseudacorus, to elucidate their structures, to determine the contents in roots and aerial parts (stems, leaves) of the plant, and to study their antitumor activity in vitro.…”

“…The principal metabolites from roots were isoflavonoids 8 and 11 and gentisic acid (3), the contents of which were 1.510 -2 , 0.9510 -2 , and 1.310 -2 %, respectively, of the air-dried root biomass ( Table 2). The contents of polyphenols 4-7, 14, and 16 [1,5] in roots were less and made up thousandths of a percent. The composition of flavonoids and isoflavonoids in the aerial plant part was more varied than that in the roots [5].…”

“…The contents of polyphenols 4-7, 14, and 16 [1,5] in roots were less and made up thousandths of a percent. The composition of flavonoids and isoflavonoids in the aerial plant part was more varied than that in the roots [5]. The content of trans-3-hydroxy-5,7-dimethoxyflavanone (9) [5], which was not identified in roots, was the greatest (0.8810 -2 %) in I. pseudacorus stems.…”

“…Column chromatography over silica gel and Sephadex LH-20 isolated from CHCl 3 and hexane extracts of I. pseudacorus roots the isoflavonoids irilin B (8) [5], 5,7-dihydroxy-2c,6-dimethoxyisoflavone (11) [5], ayamenin A (16) [5], and the flavonoid 3,6-dimethoxy-5,7-dihydroxyflavone (14) [5]. Chromatography of the EtOH extract over Toyopearl HW-50 followed by purification over reversed-phase C-18 adsorbent isolated gallic (1) [6], 2,3,5-trihydroxybenzoic (2) [7], gentisic (3) [8], and p-hydroxybenzoic (4) acids [9] in addition to the new metabolite 5.…”