Polyphenolic Profile, Anti-Inflammatory and Antinociceptive Activity of an Extract from Arctium lappa L. Roots

Conea, S.; Mogoşan, Cristina; Voștinaru, Oliviu; Toma, Claudia Crina; Hepcal, Ioana Cuc; Cazacu, Irina; Pop, Cristina; Vlase, Laurian

doi:10.15835/nbha45110549

Cited by 11 publications

(8 citation statements)

References 22 publications

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“…The antinociceptive activity was evaluated on a model of inflammatory pain induced with λ-carrageenan in rats, by a modified method of Randall and Selitto [ 30 , 31 ]. The oral administration of the synthesized compounds 5a – l in doses of 50 mg/kg bw, produced a good increase of nociceptive threshold when compared to negative control group.…”

Section: Resultsmentioning

confidence: 99%

Heterocycles 48. Synthesis, Characterization and Biological Evaluation of Imidazo[2,1-b][1,3,4]Thiadiazole Derivatives as Anti-Inflammatory Agents

et al. 2018

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Non-steroidal anti-inflammatory drugs (NSAIDs) are an important pharmacological class of drugs used for the treatment of inflammatory diseases. They are also characterized by severe side effects, such as gastrointestinal damage, increased cardiovascular risk and renal function abnormalities. In order to synthesize new anti-inflammatory and analgesic compounds with a safer profile of side effects, a series of 2,6-diaryl-imidazo[2,1-b][1,3,4]thiadiazole derivatives 5a–l were synthesized and evaluated in vivo for their anti-inflammatory and analgesic activities in carrageenan-induced rat paw edema. Among all compounds, 5c showed better anti-inflammatory activity compared to diclofenac, the standard drug, and compounds 5g, 5i, 5j presented a comparable antinociceptive activity to diclofenac. None of the compounds showed ulcerogenic activity. Molecular docking studies were carried out to investigate the theoretical bond interactions between the compounds and target, the cyclooxygenases (COX-1/COX-2). The compound 5c exhibited a higher inhibition of COX-2 compared to diclofenac.

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Section: Resultsmentioning

confidence: 99%

Heterocycles 48. Synthesis, Characterization and Biological Evaluation of Imidazo[2,1-b][1,3,4]Thiadiazole Derivatives as Anti-Inflammatory Agents

et al. 2018

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“…In addition, extracts of A. lappa presented anti‐allergic effect by inhibiting degranulation of human basophils in vitro and acute skin response in vivo . Also, a polysaccharide fraction of A. lappa extracts showed a dose dependent anti‐edematogenic activity and an antinociceptive effect on carrageenan‐induced paw edema in mice . In addition, supercritical extracts from A. lappa leaves were able to inhibit the classical and alternative pathways, as well as the lectin pathway.…”

Section: Discussionmentioning

confidence: 95%

Inhibitory Effect of Supercritical Extracts from Arctium lappa L. on the Lectin Pathway of the Complement System

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et al. 2019

Chemistry & Biodiversity

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The complement system participates in host defense by eliminating microorganisms and triggering inflammation. However, insufficient control or exacerbated complement activation contributes to inflammatory diseases. Since promising antioxidant and anti-inflammatory activities have been identified in Arctium lappa L. extracts, this study aims to explore the effect of A. lappa extracts on the lectin pathway (LP) of complement activation. Four extracts were obtained by supercritical extraction using scCO 2 with or without ethanol as cosolvent, at different temperatures and pressures (E1: 2.2 mg/mL, E2: 2.6 mg/mL and E3: 2.0 mg/mL, E4: 1.5 mg/ mL). To evaluate the effect of A. lappa extracts on the LP activation, an ELISA assay using mannose binding lectin pathway of complement was carried out with C4 detection. All extracts showed a concentration-dependent inhibitory effect on the activation of complement by the LP. The following IC 50 were observed for E1, E2, E3 and E4: 179.4 μg/mL, 74.69 μg/mL, 119.1 μg/mL and 72.19 μg/mL, respectively. Our results suggest that A. lappa extracts are potential candidates for the treatment of inflammatory disorders that are complement-related. ResultsThe A. lappa extracts E1, E2, E3 and E4 displayed an inhibitory effect on the functional activity of lectin pathway (Figure 1). The extract E1 (at concentration Figure 1. Effect of Arctium lappa L. extracts on functional activity of the MBL lectin pathway of complement system. The activation of the lectin pathway was evaluated in the presence of four extracts obtained under different conditions: The extracts E1 and E2 were obtained using scCO 2 in the presence (A) and absence of ethanol (B) as co-solvent, respectively, at 200 bar and 60°C. The extract E3 was obtained using scCO 2 + ethanol at 150 bar and 80°C (C). The extract E4 was obtained using scCO 2 + ethanol at 250 bar and 80°C (D). The final concentration of all extracts evaluated is represented in the x axis. All extracts dilutions were compared with the positive control (NHS diluted to represent 50 % of lectin pathway activation) by one-way ANOVA and Bonferroni's multiple comparison test (***p < 0.001, **p < 0.01, and *p < 0.05). All biological experiments were performed twice in duplicate and the results were expressed as mean and standard deviation.

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“…The anti-inflammatory effect of the dry ESV was tested in five rat groups (n = 6) using a previously published method [19,20]. Rats from the control group were treated orally with saline solution while the animals from the reference group received orally a dose of 20 mg/kg diclofenac.…”

Section: Anti-inflammatory Effectmentioning

confidence: 99%

Antihyperuricemic, Anti-Inflammatory and Antihypertensive Effect of a Dry Extract from Solidago virgaurea L. (Asteraceae)

et al. 2021

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Solidago virgaurea L. is a perennial plant used in European traditional medicine as a diuretic or a remedy for inflammatory conditions of the urinary tract but also for gout, especially in the Balkans. The present study was focused on a preclinical, in vivo evaluation of antihyperuricemic, anti-inflammatory, and antihypertensive effects of a dry extract from S. virgaurea L. (ESV). Colorimetric and HPLC–MS techniques were used to identify the main chemical constituents of ESV. Antihyperuricemic effect of ESV was assessed in a rat model of hyperuricemia induced by the administration of potassium oxonate. Antihypertensive effect of ESV was evaluated in hyperuricemic rats by monitoring systolic blood pressure with a non-invasive blood-pressure recording system. The anti-inflammatory effect of ESV was tested using a rat model of paw edema. The main chemical constituents of ESV were rutin and phenolic acids represented by chlorogenic and caffeic acid. ESV demonstrated significant antihyperuricemic effects in rats due to an uricosuric mechanism. Additionally, ESV reduced the progression of arterial hypertension in hyperuricemic rats and also showed anti-inflammatory properties slightly inferior to diclofenac. The results suggest that ESV could be a natural remedy for the treatment of gout and protection against endothelial dysfunction caused by hyperuricemia.

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Polyphenolic Profile, Anti-Inflammatory and Antinociceptive Activity of an Extract from Arctium lappa L. Roots

Cited by 11 publications

References 22 publications

Heterocycles 48. Synthesis, Characterization and Biological Evaluation of Imidazo[2,1-b][1,3,4]Thiadiazole Derivatives as Anti-Inflammatory Agents

Heterocycles 48. Synthesis, Characterization and Biological Evaluation of Imidazo[2,1-b][1,3,4]Thiadiazole Derivatives as Anti-Inflammatory Agents

Inhibitory Effect of Supercritical Extracts from Arctium lappa L. on the Lectin Pathway of the Complement System

Antihyperuricemic, Anti-Inflammatory and Antihypertensive Effect of a Dry Extract from Solidago virgaurea L. (Asteraceae)

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