Polyphenolic Secondary Metabolites Synergize the Activity of Commercial Antibiotics against Clinical Isolates of β-Lactamase-producing Klebsiella pneumoniae

Dey, Diganta; Ghosh, Somsubhra; Ray, Ratnamala; Hazra, Banasri

doi:10.1002/ptr.5527

Cited by 20 publications

(13 citation statements)

References 52 publications

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“…Traditional Chinese green tea and its constituents have long been studied for antimicrobial properties. EGCG, for example, is reported to have growth inhibitory effects both against gram-positive and gram-negative bacteria, i.e., Staphylococcus aureus [ 7 , 8 , 9 ], Escherichia coli [ 10 ], Acinetobacter baumannii [ 11 ], and klebsiella pneumoniae [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

EGCG-Mediated Potential Inhibition of Biofilm Development and Quorum Sensing in Pseudomonas aeruginosa

Hao

Yang

Zhao

et al. 2021

IJMS

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Pseudomonas aeruginosa (P. aeruginosa), one of the dangerous multidrug resistance pathogens, orchestrates virulence factors production through quorum sensing (QS). Since the exploration of QS inhibitors, targeting virulence to circumvent bacterial pathogenesis without causing significant growth inhibition is a promising approach to treat P. aeruginosa infections. The present study has evaluated the anti-QS and anti-infective activity of epigallocatechin-3-gallate (EGCG), a bioactive ingredient of the traditional green tea, against P. aeruginosa. EGCG showed significant inhibitory effects on the development of biofilm, protease, elastase activity, swimming, and swarming motility, which was positively related to the production of C4-AHL. The expression of QS-related and QS-regulated virulence factors genes was also evaluated. Quantitative PCR analysis showed that EGCG significantly reduced the expression of las, rhl, and PQS genes and was highly correlated with the alterations of C4-AHL production. In-vivo experiments demonstrated that EGCG treatment reduced P. aeruginosa pathogenicity in Caenorhabditis elegans (C. elegans). EGCG increased the survival of C. elegans by 23.25%, 30.04%, and 36.35% in a dose-dependent manner. The findings of this study strongly suggest that EGCG could be a potential candidate for QS inhibition as an anti-virulence compound against bacterial infection.

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Section: Introductionmentioning

confidence: 99%

EGCG-Mediated Potential Inhibition of Biofilm Development and Quorum Sensing in Pseudomonas aeruginosa

Hao

Yang

Zhao

et al. 2021

IJMS

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“…Bacterial strains usually were clinical isolates with a specific resistance (no full antibiogram reported). In the reviewed reports, the utilization of standard strains were the exception more than the rule (3% of the tested strains) [32][33][34][35][36][37][38][39][40] . In almost all the cases, resistant opportunistic bacteria were the target, the most frequently strain tested were clinical isolates of MRSA.…”

Section: Summary Of the Literature Review Resultsmentioning

confidence: 99%

“…Table 2: Summary of purified natural products screened for antibiotic modulatory effects of commercially available drugs against nosocomial bacteria, that yielded synergistic effects. For more details see text [32][33][34]46,47,51,70,71,[97][98][99][100][101] . is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.…”

Section: (Which Was Not Certified By Peer Review)mentioning

confidence: 99%

Synergistic effects of natural products and commercial antibiotics—A mini–review 2010–2015

Nitsch-Velasquez

2020

Preprint

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Context: The antimicrobial resistant era requires advances in the approaches and technologies to find new treatments. The enhancement of the antimicrobial activity of commercially available drugs (CADs) by natural products (NPs) has successful mixtures (e.g., clavulanic acid and amoxicillin). Objective: To systematically review reports of synergistic effects of CADs and NPs against opportunistic microbial strains from 2010 to April 2016. Methods: The databases and search engines PubMed, Medline, Scifinder, Scopus, ScienceDirect, Scholar Google were systematically searched. Among the keywords utilized were: synergistic effects natural products and antibioitcs, botanicals and antibiotics bioassays, plant extracts interaction with antibioitics and antibiotic adjuvant bioassays. Only synergistic results were tabulated and analyzed according to CADs, NPs and strains. Results: A set of 76 studies that reported in vitro synergistic effects of CADs and NPs against gram−positive or gram−negative bacteria or fungi opportunistic strains was found. From the 60 reports on antibacterial adjuvants, the most frequent designs involved beta−lactamics or aminoglycosides against Methicillin Resistant Staphylococcus aureus. The assayed NPs encompassed extracts or fractions from 22 different species distributed worldwide (45% extracted with non−polar solvents) and 33 purified compounds (flavonoids, other polyphenols and alkaloids). Conclusions: NPs as potential drug hits for antimicrobial adjuvants had been found and should continue in the drug discovery pipeline. The field certainly would benefit of advances in purification technologies, especially for polar extracts and bioassay platforms.

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“…Some studies have suggested the synergism between EGCG and different antibiotics on inhibiting bacteria like methicillin-resistant S. aureus (MRSA), P. gingivalis, and K. pneumoniae [22,69,70], and on bacterial biofilms. Biofilm formation by pathogenic organisms has developed tolerance to elevated levels of antimicrobials [71].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Biofilm Formation by the Synergistic Action of EGCG-S and Antibiotics

2021

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Biofilm, a stress-induced physiological state, is an established means of antimicrobial tolerance. A perpetual increase in multidrug resistant (MDR) infections associated with high mortality and morbidity have been observed in healthcare settings. Multiple studies have indicated that the use of natural products can prevent bacterial growth. Recent studies in the field have identified that epigallocatechin gallate (EGCG), a green tea polyphenol, could disrupt bacterial biofilms. A modified lipid-soluble EGCG, epigallocatechin-3-gallate-stearate (EGCG-S), has enhanced the beneficial properties of green tea. This study focuses on utilizing EGCG-S as a novel synergistic agent with antibiotics to prevent or control biofilm. Different formulations of EGCG-S and selected antibiotics were used to study their combinatorial effects on biofilms produced by five potential pathogenic bacteria, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcusepidermidis, and Mycobacterium smegmatis. The crystal violet (CV) assay and the sensitive fluorescence-based resazurin biofilm viability assay were used to assess the biofilm production. Our results identified optimal formulation for each bacterium, effectively inhibiting biofilm formation to an extent of 95–99%. Colony-forming unit (CFU) and cell viability analyses showed a decrease of viable bacteria. These results depict the potential of EGCG-S as a synergistic agent with antibiotics and as an anti-biofilm agent.

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Polyphenolic Secondary Metabolites Synergize the Activity of Commercial Antibiotics against Clinical Isolates of β-Lactamase-producing Klebsiella pneumoniae

Cited by 20 publications

References 52 publications

EGCG-Mediated Potential Inhibition of Biofilm Development and Quorum Sensing in Pseudomonas aeruginosa

EGCG-Mediated Potential Inhibition of Biofilm Development and Quorum Sensing in Pseudomonas aeruginosa

Synergistic effects of natural products and commercial antibiotics—A mini–review 2010–2015

Inhibition of Biofilm Formation by the Synergistic Action of EGCG-S and Antibiotics

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