Polyphenolics from various extracts/fractions of red onion (Allium cepa) peel with potent antioxidant and antimutagenic activities

Singh, Brahma N.; Singh, Braj Raj; Singh, Ram Lakhan; Prakash, Dhan; Singh, Dhananjaya P.; Sarma, B. K.; Upadhyay, Garima; Singh, Harikesh Bahadur

doi:10.1016/j.fct.2009.02.004

Cited by 233 publications

(152 citation statements)

References 45 publications

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“…The process depends on the concentration of scavenger relative to deoxyribose (Gutteridge 1984). Extracts of the plants such as Moringa oleifera (Chumark et al 2008) and Allium cepa (Singh et al 2009) rich in polyphenolics have shown excellent antioxidant properties and were proven good scavengers of reactive species such as OH, O 2 and ROO. We evaluated different concentration of cyanobacterial extracts rich in polyphenols to assess shielding effect against the degenerative reaction against deoxyribose caused by the OHÁ radical in the reaction mixture.…”

Section: Resultsmentioning

confidence: 99%

“…Cyanobacteria could be one of the most potential source for the production of natural antioxidants. Phenolic acids and flavonoids from plant sources have been well-documented for their antioxidant, free-radical quenching and redox metal ion-chelating affects (Singh et al 2009(Singh et al , 2014Tutour 1990;Apati et al 2003;Hossain et al 2016) but cyanobacteria and microalgae are relatively new source for such compounds in recent years due to their potential benefits and ease of production (Guedes et al 2013;Machu et al 2015). Gallic, chlorogenic, caffeic and ferulic acids identified from plant sources have been known as strong antioxidants and free radical scavengers (Piazzon et al 2012;Babić et al 2015) but their presence in cyanobacteria is scarcely reported and this make these organisms more potential.…”

Section: Resultsmentioning

confidence: 99%

“…Their presence in each cyanobacterial strains was validated by mass spectrometric analysis (Singh et al 2009). Compounds were detected by their respective m/z values of parent and product ions.…”

Section: Qualitative Lc-msmentioning

confidence: 99%

“…Plant phenolics and flavonoids are the known antioxidants that reduce the oxidative damage inside the cells (Nordberg and Arnér 2001;Singh et al 2009;Fraga et al 2010). Because of the presence of unique phenylpropanoids, cyanobacteria could be a new source of these secondary metabolites (Xue and He 2015).…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant properties and polyphenolic content in terrestrial cyanobacteria

et al. 2017

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Cell-free extracts of twenty terrestrial cyanobacteria were evaluated for their antioxidant properties in terms of free-radical scavenging (DPPH and ABTS) and metal chelating activity and deoxyribose protection. Extract of Anabaena constricta was the most prominent antioxidant agent (IC 50 for DPPH activity 0.91 mg ml -1 , ABTS 0.23 mg ml -1 , deoxyribose protection 0.63 mg ml -1 and Fe ?2 -ion chelating 0.9 mg ml -1 ). The extracts of cyanobacterial species contained high quantity of total phenol and total flavonoid that were supposed to impart prominent antioxidant properties. Cyanobacterial species also showed fairly high PAL activity. We reported varied quantities of polyphenolics gallic, chlorogenic, caffeic, vanillic and ferulic acids and flavonoids rutin, quercetin and kaempferol in cyanobacterial extracts. The presence of these polyphenolics was linked with the free radical scavenging, metal chelating and antioxidative damage protecting properties of the organisms. Cyanobacteria are the most feasible, promising and alternative candidates for searching out new chemical leads for industrial applications in pharmaceuticals, neutraceuticals and biomolecules of importance. Presence of biomolecules such as polyphenolics and their connection with the prominent biological functions (e.g., antioxidant properties) make these organisms a potential source of secondary metabolites with predominant biological activities. Additionally, dominant presence of polyphenols as antioxidant agents in cyanobacterial species may reflect their adaptation strategies against abiotic stresses for their ecological success in different habitats.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Qualitative Lc-msmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antioxidant properties and polyphenolic content in terrestrial cyanobacteria

et al. 2017

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“…Oxidative stress that can facilitate the progression of mutagenesis or atherosclerosis (Delaney et al, 2012;Peluso et al, 2012) may be counteracted by plant polyphenols. Red onion extracts containing ferulic acid, gallic acid, 3,4-dihydroxybenzoic acid, quercetin, and kaempferol inhibited in vitro b-carotene and linoleic acid oxidation, impeded ferric ion-induced lipid peroxidation and protein fragmentation; furthermore they inhibited tobacco-induced mutagenicity in strains of Salmonella typhimurium (Singh et al, 2009). Regular consumption of dark chocolate (rich in polyphenols) by healthy humans reduced the intensity of DNA damage in peripheral blood mononuclear cells as compared with the consumption of white chocolate (Spadafranca et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of plant phenolics on fMLP-induced intracellular calcium rise and chemiluminescence of human polymorphonuclear leukocytes and their chemotactic activityin vitro

Nowak

Zasowska-Nowak

Białasiewicz

et al. 2015

Pharmaceutical Biology

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Context: Polymorphonuclear leukocytes (PMNs) produce oxidants, contributing to systemic oxidative stress. Diets rich in plant polyphenols seem to decrease the risk of oxidative stressinduced disorders including cardiovascular disease. Objective: The objective of this study was to examine the in vitro effect of each of the 14 polyphenols on PMNs chemotaxis, intracellular calcium response, oxidants production. Materials and methods: Blood samples and PMNs suspensions were obtained from 60 healthy non-smoking donors and incubated with a selected polyphenol (0.5-10 mM) or a control solvent. We assessed resting and fMLP-dependent changes of intracellular calcium concentration ([Ca 2+ ] i ) in PMNs with the Fura-2AM method and measured fMLP-induced luminol enhanced whole blood chemiluminescence (fMLP-LBCL). Polyphenol chemoattractant activity for PMNs was tested with Boyden chambers. Results: Polyphenols had no effect on resting [Ca 2+ ] i . Unaffected by other compounds, fMLPdependent increase of [Ca 2+ ] i was inhibited by quercetin and catechol (5 mM) by 32 ± 14 and 12 ± 10% (p50.04), respectively. Seven of the 14 tested substances (5 mM) influenced fMLP-LBCL by decreasing it. Catechol, quercetin, and gallic acid acted most potently reducing fMLP-LBCL by 49 ± 5, 42 ± 15, and 28 ± 18% (p50.05), respectively. 3,4-Dihydroxyhydrocinnamic, 3,4-dihydroxyphenylacetic, 4-hydroxybenzoic acid, and catechin (5 mM) revealed distinct (p50.02) chemoattractant activity with a chemotactic index of 1.9 ± 0.8, 1.8 ± 0.7, 1.6 ± 0.6, 1.4 ± 0.2, respectively. Conclusion and discussion: Catechol, quercetin, and gallic acid at concentrations commensurate in human plasma strongly suppressed the oxidative response of PMNs. Regarding quercetin and catechol, this could result from an inhibition of [Ca 2+ ] i response.

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