Polyphenols composition, antioxidant and antimicrobial properties of Pinus sylvestris L. shoots extracts depending on different drying methods

Abstract

Scots pine (Pinus sylvestris L.) shoots have been increasingly commonly used as functional food or its component, the shoots contain various active components, unknown from other raw materials. The objective of the study was to assess the influence of the drying method on the content of bioactive compounds and antioxidative and antimicrobial activity of pine shoots. It was demonstrated that freeze drying (PSL), vacuum drying (PSP) and natural drying (PSN) have significant impact on the physicochemical properties, content of bioactive compounds and antioxidative activity of the prepared ethanol-water extracts. The content of the studied compounds varied significantly in the tested shoots. In spectrophotometric testing the highest total flavonoid content was demonstrated in the PSP sample, at 5.51 mg quercetin/g dw. On the other hand, the reducing capacity was as follows: PSN>PSP>PSL in the range from 13.4 to 5.73 mg gallic acid/g dw. However in assay conducted using HPLC methods the highest content of polyphenols characterized extract from freeze-dried raw material (9151.15 µg/g), followed by vacuum-dried (8264.57 µg/g), and the lowest content of phenolic compounds was found in convection-dried shoots (7621.76 µg/g). The studied extracts demonstrated antioxidative properties, both in ferric reducing antioxidant power assay (FRAP) as well as in free radical quenching measurement (DPPH). All of the studied extracts demonstrated antimicrobial and fungicidal properties, and they were particularly efficient in the case of gram-negative bacteria.

Identification of Polyphenols from Coniferous Shoots as Natural Antioxidants and Antimicrobial Compounds

“…The tested shoots, in addition to the color, differed noticeably in length, shape and dry matter content, which had an impact on the content of bioactive compounds in the extracts. In the current study, all raw materials were dried under the same conditions, although earlier studies indicated that the drying process significantly affected the content of bioactive compounds as well as antioxidant and antimicrobial properties of coniferous shoots [8]. In the present study, distilled water was used for extraction, which allowed to obtain a high content of phenolic compounds in extracts that had high antioxidant potential, although water is not the most effective solvent.…”

“…Bark, shoots and resins were used as a panacea for, e.g., diseases of the urinary tract, digestive tract, nervous system, respiratory and skin diseases [5][6][7]. Research carried out in recent years has confirmed that compounds present in conifer shoots exhibit therapeutic effects, and shoots are rich sources of polyphenols and have antioxidant properties [8,9]. Coniferous shoots are a particularly rich source of terpenoid hydrocarbon, pinene, in the form of alpha and beta isomers, which as one of the main components of the resin belongs to water-insoluble fraction.…”

PHENOLICS CONTENT, ANTIOXIDANT POTENTIAL, Α-Glucosidase AND Α-Amylase INHIBITORY ACTIVITIES OF FOUR FOLIAR EXTRACTS FROM PINUS SPECIES

“…The abundance and diversity found in the foliar tissues of the previously analysed species of Pinus suggest that these compounds may be responsible for several of the medicinal properties recognized for them, such as the antioxidant effects of Pinus densiflora Siebold & Zucc. [15] and P. sylvestris [12], the expectorant, diuretic and antimicrobial properties of P. sylvestris [12] and the vasorelaxant effects of Pinus morrisonicola Hayata [8]. Diabetes mellitus is a metabolic disorder, in which a hyperglycaemic condition is persistent.…”

Суммарная Антиоксидантная Активность Экстрактов Хвои Сосны Сибирской И Сосны Обыкновенной

“…Результаты изучения АОА по методу FRAP и количественного содержания фенольных соединений показаны в таблице 1. Получены значения FRAP (17,52), соответствующие аналогичному показателю побегов сосны обыкновенной (21,79 -47,25) mg GAE /g, указанным в литературе [7]. Установлено, что экстракты сосны обыкновенной имеют более высокие значения обоих показателей, чем экстракты сосны сибирской.…”