Polyphenols extract from lotus seedpod (Nelumbo nucifera Gaertn.): Phenolic compositions, antioxidant, and antiproliferative activities

Abstract

Seedpod, the nonedible portion of lotus (Nelumbo nucifera Gaertn.), was reported to be rich in polyphenols. The objective of this study was to investigate the major bioactive polyphenols of the lotus seedpods. The total polyphenol content (TPC) from ethanol extract of lotus seedpod (PELS) was found to be 34.23 μg gallic acid equivalents (GAE)/mg extract. Four polyphenolic compounds were identified in the PELS, comprised of one flavan‐3‐ol (catechin) and three flavonoids (kaemferol, quercetin and hyperoside). In vitro antioxidant and antiproliferative properties of the PELS were evaluated. PELS exhibited 89.38%, 99.82%, 68.25%, and 95.82% scavenging activities against 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH), superoxide, hydroxyl, and 2,2ʹazinobis‐3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS) radicals, respectively, at 1.6 mg/ml. The Fe3+ reducing power of PELS was 0.605 at 0.32 mg/ml, which is comparable to glutathione (GSH). The PELS showed 31.79% metal chelating capacity and 87.79% inhibition of linoleic acid auto‐oxidation at 1.6 mg/ml. PELS showed cytotoxicity toward HepG2 and LNcap cell lines in vitro with IC50 values at 44.59 and 11.50 μg/ml, respectively. The findings of this study provide evidences that the inedible lotus seedpod could be a source for natural antioxidants and anticancer agents.

Green Extraction of Phenolic Compounds from Lotus Seedpod (Receptaculum Nelumbinis) Assisted by Ultrasound Coupled with Glycerol

“…Lotus ( Nelumbo nucifera Gaertn. ) is a perennial freshwater plant widely cultivated in Asia and most parts are edible, so in China, they have been used for pharmaceutical purpose [ 1 , 2 , 3 , 4 ]. Lotus seedpod (LSP) is one of the main byproducts of lotus, which is discarded directly and leads to a large quantity of waste during the lotus seed processing [ 5 ].…”

Pink Lotus Essential Oil and Alleviates on Free Fatty Acid Induced Steatosis in HepG2 Cells via PI3K/Akt and NF-κB Pathways

“…ACC catalyzes malonyl-CoA, an intermediate metabolite of lipid formation and oxidation 22) . FAS is a rate-limiting enzyme to catalyze the final step of lipid synthesis in the liver 23) . SCD-1 is another key enzyme involving in lipid synthesis, which catalyzes fatty acid elongation and desaturation 24) .…”

Lotus ( Nelumbo nucifera ) seedpod extract inhibits cell proliferation and induces apoptosis in non‐small cell lung cancer cells via downregulation of Axl

“…Lotus (Nelumbo nucifera) is perennial aquatic herb and has been used as health food, tea, or traditional medicine. Diverse extracts and phytochemicals such as alkaloids, phenolic compounds, flavonoids, glycosides, and terpenoids isolated from lotus have reported to possess antioxidant, anti-depressive, antidiabetic, anti-angiogenic, anti-inflammatory, and anticancer activities (Chang et al, 2016;Liu et al, 2004;Moon et al, 2019;Mukherjee et al, 1996Mukherjee et al, , 1997Shen et al, 2019;Sim et al, 2019). In this study, we examined the cytotoxic effect of lotus seedpod extract (LSPE) in non-small cell lung cancer (NSCLC) cells.…”

“…Previous reports have demonstrated that various bioactive compounds including alkaloids, flavonoids, and terpenoids were isolated from these parts of lotus and exhibited multiple potentials for health beneficial or therapeutic agents (Chen et al., 2019; Cho et al., 2019; Shen et al., 2019). Although lotus seedpod is not edible, it also contains phenolic acids and flavonoids, which have been demonstrated to have antioxidant, anti‐inflammatory, and anticancer activities (Duan et al., 2016; Liao & Lin, 2012; Shen et al., 2019). While accumulating evidences demonstrated the effect of lotus seedpod extract (LSPE) and the underlying molecular mechanisms in many different cells, the information is still limited.…”