Polyphenols from<i>Prunus mume</i>: extraction, purification, and anticancer activity

Zhao, Fengyi; Du, Lizhong; Wang, Jialuan; Liu, Hongxia; Zhao, Huifang; Lyu, Lianfei; Wang, Weifan; Wang, Wenlong; Li, Weilin

doi:10.1039/d3fo01211e

Cited by 3 publications

(5 citation statements)

References 44 publications

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“…The procedure for extracting HPs [ 23 , 24 ] with ultrasound assistance proceeded as follows: initially, dried honeysuckle buds and flowers underwent pulverization using a DFT-250 portable high-speed universal pulverizer (manufactured by Linda Machinery Co., Ltd., Wenling, Wenzhou, China). Subsequently, honeysuckle bud and flower powder was added to 70% methanol and maintained a material–liquid ratio of 1:20.…”

Section: Methodsmentioning

confidence: 99%

Chemical Characterization of Honeysuckle Polyphenols and Their Alleviating Function on Ultraviolet B-Damaged HaCaT Cells by Modulating the Nrf2/NF-κB Signaling Pathways

Zheng,

Wang,

Chi

et al. 2024

Antioxidants

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Scientific evidence attests that the epidermis receives excessive ultraviolet B (UVB) radiation, triggering the generation of substantial quantities of reactive oxygen species (ROS), which disrupted the delicate equilibrium of oxidation–reduction, leading to oxidative stress and inflammation. The historical use of honeysuckle polyphenols (HPs) has garnered our attention due to their efficacy in inhibiting oxidative damage. In this study, HPs were prepared from honeysuckle flowers employing an ultrasonic-assisted extraction method and quantitatively analyzed by a LC–MS/MS, and the mechanisms underlying HPs’ antioxidative and anti-inflammatory effects on a UVB-irradiated HaCaT cell model were systematically investigated. The results showed that HPs had a significant cellular repair effect on UVB-irradiated HaCaT cells (p < 0.001). The mechanism of action indicated that HPs could allow Nrf2 to enter the nucleus by regulating the dissociation of Nrf2 from Keap1, which further increases the activity of downstream proteases (SOD and CAT), increases ROS scavenging, and reduces the intracellular malondialdehyde (MDA) level. In addition, HPs could down-regulate Toll-like receptor 4 (TLR4) and inhibit NF-κB (P65) dissociating from IκBα, resulting in a decrease in NF-κB (P65) entry into the nucleus and a decrease in inflammatory factors (TNF-α, IL-6, and IL-1β). In addition, four key compounds in HPs, including chlorogenic acid, quercetin, isorhamnetin, and luteolin, were selected to verify the mechanism of HPs repairing UVB damage using molecular docking techniques. The experiment suggested that four key active compounds could effectively occupy the Kelch homologue (Kelch) structural domain of Keap1, competitively bind with Nrf2, and facilitate the promotion of Nrf2 binding, ultimately enhancing the translocation of Nrf2 into the nucleus. In addition, four key active compounds could effectively interact with NF-κB (P65) through hydrogen bonding, van der Waals forces, and electrostatic forces to inhibit its entry into the nucleus. In summary, HPs can effectively repair the damage of HaCaT cells by UVB radiation and can be used to develop health and cosmetic products for the treatment of UV radiation-induced diseases.

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Section: Methodsmentioning

confidence: 99%

Chemical Characterization of Honeysuckle Polyphenols and Their Alleviating Function on Ultraviolet B-Damaged HaCaT Cells by Modulating the Nrf2/NF-κB Signaling Pathways

Zheng,

Wang,

Chi

et al. 2024

Antioxidants

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“…As per our previous report, polyphenol extract may interact with DNA in an intercalation mode to change or destroy DNA and cause apoptosis, and inhibit cell proliferation. When DNA damage is introduced into cells from exogenous or endogenous sources there is an increase in the amount of intracellular reactive oxygen species (ROS) that may be related to apoptosis [42]. EA is a kind of natural polyphenol, the further possible mechanism may be that EA can induce apoptosis by interacting with DNA in an intercalation mode, changing or destroying DNA.…”

Section: Discussionmentioning

confidence: 99%

“…(IC50 for nonmalignant HUVECs)/(IC50 for the human tumor cell lines)) [42], as illustrated in Figure 8c. An important consideration for future pharmacological applications is the SI.…”

Section: Anticancer Activity Of Ea In Vitromentioning

confidence: 95%

“…The highest inhibition rate was observed in HeLa cells with an IC 50 of 35 µg/mL, while in MCF-7 cells, the IC 50 was 73 µg/mL, in HepG2 cells, the IC 50 was 213 µg/mL, in A549 cells, the IC 50 was 242 µg/mL, and in HUVECs, the IC 50 was 258 µg/mL (Figure 8b). The toxicity of EA to the nonmalignant cell line (HUVECs) was also investigated to characterize the selectivity, which is expressed as the selectivity index (SI) (SI = (IC 50 for nonmalignant HUVECs)/(IC 50 for the human tumor cell lines)) [42], as illustrated in Figure 8c. An important consideration for future pharmacological applications is the SI.…”

Section: Anticancer Activity Of Ea In Vitromentioning

confidence: 99%

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Ellagic Acid from Hull Blackberries: Extraction, Purification, and Potential Anticancer Activity

Wang,

Zhao,

et al. 2023

IJMS

Self Cite

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Ellagic acid (EA) is present at relatively high concentrations in many berries and has many beneficial health effects, including anticancer properties. To improve the development and utilization of blackberry fruit nutrients, we divided Hull blackberry fruits into five growth periods according to color and determined the EA content in the fruits in each period. The EA content in the green fruit stage was the highest at 5.67 mg/g FW. Single-factor tests and response surface methodology were used to optimize the extraction process, while macroporous resin adsorption and alkali dissolution, acid precipitation, and solvent recrystallization were used for purification. The highest purity of the final EA powder was 90%. The anticancer assessment results determined by MTT assay showed that EA inhibited HeLa cells with an IC50 of 35 μg/mL, and the apoptosis rate of the cells increased in a dose-dependent manner, with the highest rate of about 67%. We evaluated the changes in the mRNA levels of genes related to the EA-mediated inhibition of cancer cell growth and initially verified the PI3K/PTEN/AKT/mTOR pathway as the pathway by which EA inhibits HeLa cell growth. We hope to provide a theoretical basis for the deep exploration and utilization of this functional food.

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“…Referred to our previous report [67], pretreatment cells (Hela, HepG2, MCF-7, A549, and HUVEC cells) were incubated with BPI, prolamin, glutelin, albumin, and globulin at different concentrations for 48 h at 5% CO 2 and 37 • C, with the process being repeated three times for each concentration. The following treatment was consistent with the abovementioned reports.…”

Section: Anticancer Activities and Cytotoxicity Assay In Vitromentioning

confidence: 99%

Proteins from Blackberry Seeds: Extraction, Osborne Isolate, Characteristics, Functional Properties, and Bioactivities

Wang,

Zhao,

et al. 2023

IJMS

Self Cite

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Blackberry fruit contains high levels of nutrients and phenolic compounds. Blackberry pomace accounts for 20~30% of its whole fruit during processing and is generally treated as fertilizer. Blackberry pomace has many seeds that contain carbohydrates, polyphenols, flavonoids, pectin, protein, and other bioactive nutrients. However, its functional properties and seed protein compositions have not been reported. We used a single-factor experiment, response surface, and Osborne isolate method to extract protein isolate, albumin, globulin, glutelin, and prolamin from blackberry seeds for the first time and evaluated their characteristics and functional properties. Glutelin and protein isolate showed good water-holding capacity, emulsification, and foaming capacity, while albumin and globulin showed good oil-holding capacity and thermal stability. They were found to have good antioxidant activities that might be good DPPH free radical scavengers, especially prolamin, which has the lowest IC50 value (15.76 μg/mL). Moreover, globulin had the lowest IC50 value of 5.03 μg/mL against Hela cells, 31.82 μg/mL against HepG2 cells, and 77.81 μg/mL against MCF-7 cells and a high selectivity index (SI), which suggested globulin had better anti-cervical, antihepatoma, and anti-breast activity but relatively low cytotoxicity. These seed proteins may have great prospects for the development and application of food and drugs in the future.

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Polyphenols fromPrunus mume: extraction, purification, and anticancer activity

Abstract: Prunus mume is one of the most ancient medicinal herbs and health foods commonly used in Asian countries with high nutritional ingredients and biological activities. Polyphenols are important functional components...

Cited by 3 publications

References 44 publications

Chemical Characterization of Honeysuckle Polyphenols and Their Alleviating Function on Ultraviolet B-Damaged HaCaT Cells by Modulating the Nrf2/NF-κB Signaling Pathways

Chemical Characterization of Honeysuckle Polyphenols and Their Alleviating Function on Ultraviolet B-Damaged HaCaT Cells by Modulating the Nrf2/NF-κB Signaling Pathways

Ellagic Acid from Hull Blackberries: Extraction, Purification, and Potential Anticancer Activity

Proteins from Blackberry Seeds: Extraction, Osborne Isolate, Characteristics, Functional Properties, and Bioactivities

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