Polyphenon Extraction Process From In vitro Culture of Camellia Sinensis L Callus With Ethyl Alcohol

Abstract

The purpose of this study was to obtain a polyphenon profile from callus extract obtained from in vitro culture of Camellia sinensis L. Polyphenon is one of the bioactive compounds found in Camellia sinensis L plants which can also be produced through in vitro culture. Polyphenon as a bioactive is often used in various fields including in the fields of industry: manufacturing, food-drinks, health, agriculture, computers. To get a certain amount of polyphenon from in vitro culture, Camellia sinensis L callus was first extracted with water solvent, as well as non-water solvents / organic solvents such as ethyl alcohol. The methods used were: (1) inoculation of leaf explants from Camellia sinensis L in vitro on the media plus growth regulators to form callus, (2) treatment to be able to be reused (3) reap the callus followed by callus weighing and callus observation. (3) extraction and isolation of bioactive polyphenon (4) identification of polyphenon from Camellia sinensis L callus with high-performance liquid chromatography qualitatively and quantitatively. The results obtained are a form of polyphenon chromatogram profile from callus Camellia sinensis L which resembles a standard chromatogram of polyphenon.