Polyphyllin VII induces fibroblasts apoptosis via the ERK/JNK pathway

Context Polyphyllin II (PPII) is a steroidal saponin isolated from Rhizoma Paridis . It exhibits significant antitumor activity such as anti-proliferation and pro-apoptosis in lung cancer. Objective To explore whether PPII induce autophagy and the relationship between autophagy and apoptosis in non-small cell lung cancer (NSCLC) cells. Materials and methods The effects of PPII (0, 1, 5, and 10 μM) were elucidated by CCK8 assay, colony formation test, TUNEL staining, MDC method, and mRFP-GFP-LC3 lentivirus transfection in A549 and H1299 cells for 24 h. DMSO-treated cells were selected as control. The protein expression of autophagy (LC3-II, p62), apoptosis (Bcl-2, Bax, caspase-3) and p-mTOR was detected by Western blotting. We explored the relationship between autophagy and apoptosis by autophagy inhibitor CQ (10 μM) and 3-MA (5 mM). Results PPII (0, 1, 5, and 10 μM) inhibited the proliferation and induced apoptosis. The IC 50 values of A549 and H1299 cells were 8.26 ± 0.03 and 2.86 ± 0.83 μM. We found that PPII could induce autophagy. PPII promoted the formation of autophagosome, increased the expression of LC3-II/LC3-I ( p < 0.05), while decreased p62 and p-mTOR ( p < 0.05). Additionally, the co-treatment with autophagy inhibitors promoted the protein expression of c-caspase-3 and rate of Bax/Bcl-2 ( p < 0.05), compared with PPII-only treatment group. Therefore, our results indicated that PPII-induced autophagy may be a mechanism to promote cell survival, although it can also induce apoptosis. Conclusions PPII-induced apoptosis exerts its anticancer activity by inhibiting autophagy, which will hopefully provide a prospective compound for NSCLC treatment.

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“… 2021 ; Zhong et al. 2021 ). PPII has been reported to induce the anti-proliferation and pro-apoptosis effects of NSCLC cells (Niu et al.…”

Section: Discussionmentioning

confidence: 99%

Polyphyllin II induced apoptosis of NSCLC cells by inhibiting autophagy through the mTOR pathway

Jiao

Ming

et al. 2022

Pharmaceutical Biology

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“…ese 4 compounds not only relieved the degeneration and Polyphyllin D hampered vasculogenic mimicry (VM) formation, an indication of cancer metastasis, through inhibition of the PI3K-Akt-Twist1-VE-cadherin pathway [41] necrosis of liver tissue but also reduced the degree of fibroplastic proliferation via the suppression of VEGF, ERK1/2, RASAL1, PDGF, and α-SMA [12,51,52]. Furthermore, polyphyllin G was also shown to induce the apoptosis of hypertrophic scar fibroblasts through the modulation of the ERK/JNK pathway [53]. Collectively, these results indicate that PS is a class of potential antifibrosis agents, which deserve future serious investigation.…”

Section: Antifibrosismentioning

confidence: 99%

Chemical Constituents and Pharmacological Activities of Steroid Saponins Isolated from Rhizoma Paridis

Liu

Tian

et al. 2021

Journal of Chemistry

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Rhizoma Paridis, the rhizome of liliaceous plants Paris polyphylla, is one of the most commonly used herbal drugs in China. Phytochemical and pharmacological studies have shown that steroid saponins were the major effective ingredients of Rhizoma Paridis to exert antitumor, anti-inflammatory, hemostasis, and antifibrosis functions. In this review, we discussed the chemical structures of steroid saponins and their related biological activity and mechanisms in cellular and animal models, aiming to provide a reference for future comprehensive exploitation and development of saponins.

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Paris saponins Ⅶ inhibits glycolysis of ovarian cancer via the RORC/ACK1 signaling pathway

Yuan

Zhang

et al. 2023

Biochemical Pharmacology

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Polyphyllin VII induces fibroblasts apoptosis via the ERK/JNK pathway

Cited by 4 publications

References 43 publications

Polyphyllin II induced apoptosis of NSCLC cells by inhibiting autophagy through the mTOR pathway

Polyphyllin II induced apoptosis of NSCLC cells by inhibiting autophagy through the mTOR pathway

Chemical Constituents and Pharmacological Activities of Steroid Saponins Isolated from Rhizoma Paridis

Paris saponins Ⅶ inhibits glycolysis of ovarian cancer via the RORC/ACK1 signaling pathway

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