2007
DOI: 10.1002/jgm.1035
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Polyplex‐mediated gene transfer and cell cycle: effect of carrier on cellular uptake and intracellular kinetics, and significance of glycosaminoglycans

Abstract: The data obtained indicates that no single parameter (pinocytosis, cell surface GAGs, nuclear uptake) solely accounts for the differential pDNA uptake or expression during cell cycle, and that the main difference in PLL- and PEI-mediated transfections seems to be at the nuclear level.

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Cited by 62 publications
(49 citation statements)
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“…Our results are somewhat contradictory with the results reported in the literature: PEI complexes have shown to be less resistant to the effects of GAGs compared to PLL complexes [34,35]. The different results may be explained by the polydispersity of our PLL/DNA complexes.…”
Section: Agarose Gel Electrophoresiscontrasting
confidence: 84%
“…Our results are somewhat contradictory with the results reported in the literature: PEI complexes have shown to be less resistant to the effects of GAGs compared to PLL complexes [34,35]. The different results may be explained by the polydispersity of our PLL/DNA complexes.…”
Section: Agarose Gel Electrophoresiscontrasting
confidence: 84%
“…The structure also leads to to bulkier complexes compared to PEI, which has been indicated experimentally 11,19 as well.…”
Section: Discussionmentioning
confidence: 64%
“…Out of the two, PEI is often considered more promising due to its ability to change its protonation state under physiological pH range. A host of both experimental 4,[17][18][19][20] and computational work 21-27 on these complexes exists. That said, response of the DNA-polycation complexes to increasing concentrations of monovalent and divalent ions has not been compared earlier via simulations.…”
Section: Introductionmentioning
confidence: 99%
“…These attempts at improving the transport of DNA to the nucleus have however met with limited success. 9,11,[17][18][19][20][21][22][23][24] . Also, cell division had a positive influence on the nuclear inclusion of colloidal gold particles 25 .…”
mentioning
confidence: 99%