Polyploid Genome Structure of &lt;i&gt;Drosera spatulata&lt;/i&gt; Complex (Droseraceae)

Shirakawa, Jun; Nagano, Katsuya; Hoshi, Yoshikazu

doi:10.1508/cytologia.77.97

Cited by 11 publications

(10 citation statements)

References 31 publications

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“…The highest percentage of similarity between D. rotundifolia and D. tokaiensis seems to show a close relationship among the three species. However, previous molecular and cytogenetic works clearly demonstrated that D. tokaiensis was an allopolyploid (2n=6x= 60, hexaploid) with hybrid origin between D. rotundifolia (2n=2x=20, diploid) as the paternal ancestor and D. spatulata (2n=4x= 40, tetraploid) as the maternal ancestor (Hoshi et al 2008, Hoshi et al 2010, Shirakawa et al 2012.…”

Section: Resultsmentioning

confidence: 96%

“…In Japan, this species occurs in the main Islands except the Ryukyu Islands (Horikawa 1976, Nakano et al 2004. The leaves of the species are spoon-shaped (Nakano et al 2004, Shirakawa et al 2012, Hoyo and Tsuyuzaki 2013, and all plants are the diploid with middle size (2-3 µm) of metaphase chromosomes (2n=2x=20M) (Fig. 1A).…”

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confidence: 99%

“…1C). The parental ancestors of D. tokaiensis are D. rotundifolia as the paternal origin and D. spatulata as the maternal origin (Shirakawa et al 2012) (Fig. 2).…”

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confidence: 99%

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RAPD Profiling of Three Japanese Drosera Species

et al. 2015

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Summary RAPD analysis was carried out using 1200 decamer random sequences to clarify the whole fingerprint of DNA fragment appearance patterns among three Japanese Drosera species: D. tokaiensis, D. rotundifolia and D. spatulata. The RAPD analysis results showed that D. tokaiensis had not only magnificent bands common to other two species, but also many specific bands, although D. tokaiensis is of amphiploidal origin between D. rotundifolia and D. spatulata. The specific bands of D. tokaiensis might generate after the speciation or amphiduplication. Therefore, the relationship of genome compositions among the three species suggested that RAPD fragments were preferentially amplified from 20 middle-sized chromosomes in D. rotundifolia and D. tokaiensis. Thus, the chromosome information and the RAPD profiling suggested that alloploidal genome formation during Drosera speciation might obtain new beneficial genetic characters to survive and adapt to certain environments.

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Section: Resultsmentioning

confidence: 96%

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confidence: 99%

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RAPD Profiling of Three Japanese Drosera Species

et al. 2015

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“…After about 10 d, the primary roots growing straight were collected and treated with 0.05% colchicine for 10 h, fixed in a fixative mixed with ethanol-acetic acid-chloroform (2 : 1 : 1) and then stored in a deep freezer until use. Fluorescent banding using CMA and 4′,6-diamidino-2-phenylindole (DAPI) was adapted to plant chromosomes by Schweizer (1976) and is effective for karyotype analysis in other various plant species (Hoshi et al 2008, Urdampilleta et al 2008, Zaman and Alam 2009, Begum et al 2010, Fawzia and Alam 2011, Hoshi et al 2011, Shahla and Alam 2011, Shirakawa et al 2012, Kuroki et al 2013. The fluorescent banding procedure simplified and adapted for conifer chromosome analysis by Kondo and Hizume (1982) was used.…”

Section: Methodsmentioning

confidence: 99%

Fluorescent Chromosome Banding Patterns in Six Species of Abies, Pinaceae

2016

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SummaryThe six Abies species, A. laciocarpa, A. veitchii, A. sachalinensis, A. mariesii, A. faxoniana and A. georgei, were investigated for their chromosomes by the fluorescent banding method using fluorochromes chromomycin A 3 (CMA) and 4′,6-diamidino-2-phenylindole (DAPI). All six species have 2n=24 chromosomes and a similar karyotype consisting of seven pairs of long metacentric chromosomes and five pairs of shorter submetaand subtelocentric chromosomes, supporting previous studies. Eight clear CMA-bands appeared at the interstitial region of one arm of long metacentric chromosomes in all species, and in A. veitchii and A. sachalinensis, their number varied from six to eight among plants and/or populations. A weak CMA-band appeared at the interstitial region of one chromosome pair, while a weak CMA-band appeared at the proximal region in some species. In most species DAPI did not produce clear bands, and sites of clear CMA-bands were DAPI negative. Only A. mariesii showed many DAPI-bands at the interstitial and/or centromeric regions of several chromosomes. A few weak DAPI-bands appeared in some other species. The fluorescent banding and FISH patterns reported in Abies species were compared and discussed with taxonomic treatment and molecular phylogeny of Abies.

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“…Fluorescence in situ hybridization (FISH) has been useful to detect DNA distribution in nuclei and on chromosomes (Hoshi et al 2011, Shibata and Hizume 2011, Ebadi-Almas et al 2012, Shirakawa et al 2012, Suto et al 2012, Ikeda et al 2013, Kuroki et al 2013, Ruan et al 2013, Yokomi et al 2013, Suto et al 2013, Lombello and Pinto-Maglio 2014, Abo-Zeid et al 2015, Kantek et al 2015, Shibata and Hizume 2015, Matsunaga 2016). However, FISH supplies only a snapshot of subnuclear dynamics because it requires the fixation of cells and DNA denaturation with high temperature for hybridization.…”

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Chromatin Live Imaging with Genome Editing Techniques: Switching from Scissors to a Lamp

Fujimoto

Matsunaga

2016

CYTOLOGIA

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Summary Chromatin live imaging integrated with programmable DNA binding proteins derived from genome editing methods opens up the era of spatio-temporal analyses of chromatin dynamics. The DNA binding proteins, including a transcription activator-like effector (TALE) or a nuclease-dead CRISPR-associated protein 9 (dCas9), can be freely designed to bind a specific DNA sequence. Instead of a nuclease for DNA cleavage, a fluorescent protein is fused to TALE or dCAS9 for visualization of chromatin. They enable us to observe the endogenous genomic loci without the fixation of cells, the denaturation of DNA for hybridization and the insertion of exogenous DNA sequences into targeted loci. Using these live imaging systems dependent on the interaction between cis endogenous DNA sequence and trans DNA binding proteins, we can analyze chromatin dynamics in living cells. Multicolor chromatin imaging based on different dCas9 proteins will be a powerful cytogenetical technique instead of multicolor FISH and chromosome painting.

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Polyploid Genome Structure of <i>Drosera spatulata</i> Complex (Droseraceae)

Cited by 11 publications

References 31 publications

RAPD Profiling of Three Japanese <i>Drosera</i> Species

RAPD Profiling of Three Japanese <i>Drosera</i> Species

Fluorescent Chromosome Banding Patterns in Six Species of <i>Abies</i>, Pinaceae

Chromatin Live Imaging with Genome Editing Techniques: Switching from Scissors to a Lamp

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