Polyploid induction of Eucalyptus dunnii Maiden to generate variability in breeding programs

Castillo, A.; López, Valeria; Tavares, Elena; Santiñaque, Federico F.; Rizza, Marco Dalla

doi:10.31285/agro.24.381

Cited by 6 publications

(7 citation statements)

References 21 publications

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“…Most of the previous studies on tetraploids focused on somatic chromosome doubling in Eucalyptus ( Silva et al, 2019 ; Castillo et al, 2020 ; Moura et al, 2020 ). Induced zygotic chromosome doubling has been successfully applied in several plants ( Randolph, 1932 ; Atwood, 1936 ; Guo et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

Liu

Wang²,

Qiu³

et al. 2022

Front. Plant Sci.

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Improvements in plant growth can bring great benefits to the forest industry. Eucalyptus urophylla is an important plantation species worldwide, and given that ploidy increases are often associated with plant phenotype changes, it was reasoned that its polyploidization may have good prospects and great significance toward its cultivation. In this study, the zygotic development period of E. urophylla was observed through paraffin sections, and a correlation between the development time of flower buds after pollination and the zygotic development period was established. On this basis, it was determined that the 25th day after pollination was the appropriate time for a high temperature to induce zygotic chromosome doubling. Then tetraploid E. urophylla was successfully obtained for the first time through zygotic chromosome doubling induced by high temperature, and the appropriate conditions were treating flower branches at 44°C for 6 h. The characterization of tetraploid E. urophylla was performed. Chromosome duplication brought about slower growing trees with thicker leaves, larger cells, higher net photosynthetic rates, and a higher content of certain secondary metabolites. Additionally, the molecular mechanisms for the variation in the tetraploid’s characteristics were studied. The qRT-PCR results showed that genes mediating the tetraploid characteristics showed the same change trend as those of the characteristics, which verified that tetraploid trait variation was mainly caused by gene expression changes. Furthermore, although the tetraploid had no growth advantage compared with the diploid, it can provide important germplasm resources for future breeding, especially for the creation of triploids.

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Section: Discussionmentioning

confidence: 99%

Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

Liu

Wang²,

Qiu³

et al. 2022

Front. Plant Sci.

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“…Similar results were recently obtained using different polyploidy inducers in two Eucalyptus species. Tetraploid and mixoploid plants were generated using trifluralin and oryzalin on E. urophylla clones (Moura et al 2020), and colchicine on Eucalyptus dunnii Maiden clones (Castillo et al 2020). Variation in chromosome number -mixoploidy -is not desirable, since it may compromise the fertility and uniformity of plants, depending on its extension (Pereira et al 2012), though the use of mixoploid plants in the field can be feasible.…”

Section: Resultsmentioning

confidence: 99%

Assessment of the cytogenetics and leaf anatomy of synthetic polyploids of Eucalyptus clones

Souza

Macedo

Ferreira

et al. 2023

Crop Breed. Appl. Biotechnol.

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The induction of polyploidy in Eucalyptus can lead to higher-yielding and more adapted clones, with better wood quality. After artificial polyploidization, it is necessary to certify the effectiveness of the process through cytogenetic analyses and anatomical trait evaluations. Thus, the aim of this study was to certify the chromosome duplication in artificially polyploidized clones of Eucalyptus grandis × Eucalyptus urophylla using cytogenetic analysis, flow cytometry, and leaf anatomy measurements. Chromosomal counts and DNA content estimation allowed the identification of artificially polyploidized clones and mixoploids. Polyploid clones had larger stomata diameter than diploids. The increase in stomata size was accompanied by lower stomatal density in most polyploid clones. The typical gigas effect of polyploidized plants was demonstrated by the expressive increase in leaf area in polyploid individuals compared to diploids. Our findings indicate that the use of polyploidy in Eucalyptus breeding is promising, allowing the discrimination of diploid, tetraploid, and mixoploid plants.

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“…Los resultados de esta investigación son importantes ya que a diferencia de muchas especies actualmente no se cuenta con estudios de caracterización morfológica de C. arborea; W. mariquitae; V. triphyllum y L. subseriata, probablemente obedezca a la bioeconomía presente en la región, la cual concentra sus esfuerzos económicos y de investigación generando paquetes tecnológicos para especies introducidas y con alto valor comercial como es el caso de pino o eucalipto de las que se obtiene materia prima para diversos productos para la industria maderera y de papel (Pérez Reyes et al, 2020;Rizza, 2020).…”

Section: Discussionunclassified

Morphological traits of native species with potential for High Andean agroecological processes - Nariño, Colombia

Acosta¹

2022

Rev. Investig. Altoandin.

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The intense production systems present in the Andean region have generated serious disturbances to high mountain ecosystems, which provide ecosystem services of great importance such as minerals, flora, and fauna. The study of the native flora through morphological descriptors becomes relevant to characterize and identify the vegetation that could be used in agroecological systems. This could serve as a strategy for the recovery and sustainable use of degraded systems since they promote diversity and responsible use of natural resources. The objective of this research was to determine the foliar morphological patterns that characterize four native shrub species of the High Andean forest: C. arborea; W. mariquitae; V. triphyllum y L. Subseriata. Fifteen qualitative and quantitative morphological leaf descriptors were used. Data were subjected to multivariate analysis of main components and multiple correspondences for quantitative and qualitative variables, respectively. Leaf width and leaf shape were found among the most relevant descriptors for C. arborea; leaf length and main petiole length for L. Subseriata; leaf width and color on the upper leaf side for W. mariquitae; and leaf width and petiole thickness for V. triphyllum. This work contributes to identifying the most relevant morphological traits of four High Andean forest shrub species with potential for the implementation of agroecological systems that contribute to the sustainability of areas disturbed by agricultural actions.

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Polyploid induction of Eucalyptus dunnii Maiden to generate variability in breeding programs

Cited by 6 publications

References 21 publications

Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

Induction and Characterization of Tetraploid Through Zygotic Chromosome Doubling in Eucalyptus urophylla

Assessment of the cytogenetics and leaf anatomy of synthetic polyploids of Eucalyptus clones

Morphological traits of native species with potential for High Andean agroecological processes - Nariño, Colombia

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