Polyploidization increases meiotic recombination frequency in Arabidopsis

Pečinka, Aleš; Wei, Fang; Rehmsmeier, Marc; Levy, Avraham A.; Scheid, Ortrun Mittelsten

doi:10.1186/1741-7007-9-24

Cited by 103 publications

(93 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…30 One should note that the majority of available data pertains to behavior of meiotic chromosomes, while studies of interphase nuclei are scarce. 34,35 Here we present the analysis of chromatin organization in relation to the number of genome copies and their origin (endopolyploidy, autopolyploidy). Our data show that, endoreplication leads to increase of spatial clustering of chromosomal domains.…”

mentioning

confidence: 99%

Spatial relationship between chromosomal domains in diploid and autotetraploidArabidopsis thaliananuclei

Sas–Nowosielska

Bernaś

2016

Nucleus

View full text Add to dashboard Cite

Polyploids constitute more than 80% of angiosperm plant species. Their DNA content is often further increased by endoreplication, which occurs as a part of cell differentiation. Here, we explore the relationship between 3D chromatin architecture, number of genome copies and their origin in the model plant, Arabidopsis thaliana. Spatial proximity between pericentromeric, interstitial and subtelomeric domains of chromosomes 1 and 4 was quantified over a range of distances. The results indicate that average nuclear volume as well as chromatin density increase with the genome copy number. Similar dependence is observed when association of homologous chromosomes (in 2C/ endopolyploid nuclei) and sister chromatid separation (in endopolyploid nuclei) is studied. Moreover, clusters of chromosomal domains are detectable at the spatial scale above microscopy resolution. Subtelomeric, interstitial and pericentromeric chromosomal domains are affected to different extent by these processes, which are modulated by endopolyploidy. This factor influences fusion of heterochromatin as well. Nonetheless, local chromatin architecture of Arabidopsis thaliana depends mainly on endopolyploidy level, and to lesser extend on polyploidy.

show abstract

mentioning

confidence: 99%

Spatial relationship between chromosomal domains in diploid and autotetraploidArabidopsis thaliananuclei

Sas–Nowosielska

Bernaś

2016

Nucleus

View full text Add to dashboard Cite

show abstract

“…TLs make it possible to identify seeds homozygous and heterozygous (or wild type) for a mutation of interest in a segregating family, so that the phenotypes of these genotypes can be compared. TLs can also be used to detect and preserve the products of rare recombination events (Melamed-Bessudo et al 2005;Shaked et al 2006;Pecinka et al 2011;Melamed-Bessudo and Levy 2012). This is particularly valuable for the creation of near-isogenic lines from mutagenized populations and for the analysis of quantitative trait loci (QTL).…”

Section: Some Uses Of Traffic Linesmentioning

confidence: 99%

“…This approach was originally developed to study recombination in Arabidopsis and involves using a pair of linked, differently colored fluorescent transgenes to mark a chromosome interval (MelamedBessudo et al 2005;Berchowitz et al 2007;Francis et al 2007;Berchowitz and Copenhaver 2008;Berchowitz and Copenhaver 2009;Pecinka et al 2011;Melamed-Bessudo and Levy 2012). The first use of this approach (MelamedBessudo et al 2005) employed fluorescent reporters (eGFP and dsRED) fused to the seed-specific napA storage protein promoter (Stuitje et al 2003).…”

mentioning

confidence: 99%

Traffic Lines: New Tools for Genetic Analysis inArabidopsis thaliana

Rossidivito

et al. 2015

Genetics

View full text Add to dashboard Cite

Genetic analysis requires the ability to identify the genotypes of individuals in a segregating population. This task is straightforward if each genotype has a distinctive phenotype, but is difficult if these genotypes are phenotypically similar or identical. We show that Arabidopsis seeds homozygous or heterozygous for a mutation of interest can be identified in a segregating family by placing the mutation in trans to a chromosome carrying a pair of seed-expressed green and red fluorescent transgenes (a "traffic line") that flank the mutation. Nonfluorescent seeds in the self-pollinated progeny of such a heterozygous plant are usually homozygous for the mutation, whereas seeds with intermediate green and red fluorescence are typically heterozygous for the mutation. This makes it possible to identify seedlings homozygous for mutations that lack an obvious seedling phenotype, and also facilitates the analysis of lethal or sterile mutations, which must be propagated in heterozygous condition. Traffic lines can also be used to identify progeny that have undergone recombination within a defined region of the genome, facilitating genetic mapping and the production of near-isogenic lines. We produced 488 transgenic lines containing single genome-mapped insertions of NAP:dsRED and NAP:eGFP in Columbia (330 lines) and Landsberg erecta (158 lines) and generated sets of traffic lines that span most regions of the Arabidopsis genome. We demonstrated the utility of these lines for identifying seeds of a specific genotype and for generating near-isogenic lines using mutations of WUSCHEL and SHOOTMERISTEMLESS. This new resource significantly decreases the effort and cost of genotyping segregating families and increases the efficiency of experiments that rely on the ability to detect recombination in a defined chromosomal segment.

show abstract

“…Comparative genetic mapping between polyploid species and their diploid relatives allows a better understanding of the evolution of genome structure and associated rearrangements. Linkage analyses have uncovered further genomic consequences of polyploidization, including increased rates of recombination in polyploid plants relative to diploid ancestors (e.g., Leflon et al, 2010;Pecinka et al, 2011;reviewed in Grandont et al, 2013) as well as homeologous (i.e., intergenomic) recombination, shown to occur in polyploid plants (Gaeta et al, 2007) and salamanders Bogart, 2006, 2010). Thus, provided one may identify the parental species and timing of hybridization events, allopolyploid lineages represent unique opportunities to address important questions regarding the reorganization of genomes that is expected to follow hybridization, and more generally to gain crucial insights on the evolutionary dynamics of genomes.…”

Section: Introductionmentioning

confidence: 99%

Origin and genome evolution of polyploid green toads in Central Asia: evidence from microsatellite markers

Betto‐Colliard¹,

Sermier²,

Litvinchuk³

et al. 2014

Heredity

View full text Add to dashboard Cite

Polyploidization, which is expected to trigger major genomic reorganizations, occurs much less commonly in animals than in plants, possibly because of constraints imposed by sex-determination systems. We investigated the origins and consequences of allopolyploidization in Palearctic green toads (Bufo viridis subgroup) from Central Asia, with three ploidy levels and different modes of genome transmission (sexual versus clonal), to (i) establish a topology for the reticulate phylogeny in a species-rich radiation involving several closely related lineages and (ii) explore processes of genomic reorganization that may follow polyploidization. Sibship analyses based on 30 cross-amplifying microsatellite markers substantiated the maternal origins and revealed the paternal origins and relationships of subgenomes in allopolyploids. Analyses of the synteny of linkage groups identified three markers affected by translocation events, which occurred only within the paternally inherited subgenomes of allopolyploid toads and exclusively affected the linkage group that determines sex in several diploid species of the green toad radiation. Recombination rates did not differ between diploid and polyploid toad species, and were overall much reduced in males, independent of linkage group and ploidy levels. Clonally transmitted subgenomes in allotriploid toads provided support for strong genetic drift, presumably resulting from recombination arrest. The Palearctic green toad radiation seems to offer unique opportunities to investigate the consequences of polyploidization and clonal transmission on the dynamics of genomes in vertebrates.

show abstract

Polyploidization increases meiotic recombination frequency in Arabidopsis

Cited by 103 publications

References 33 publications

Spatial relationship between chromosomal domains in diploid and autotetraploidArabidopsis thaliananuclei

Spatial relationship between chromosomal domains in diploid and autotetraploidArabidopsis thaliananuclei

Traffic Lines: New Tools for Genetic Analysis inArabidopsis thaliana

Origin and genome evolution of polyploid green toads in Central Asia: evidence from microsatellite markers

Contact Info

Product

Resources

About