Polyploidy‐associated genomic instability in <i>Arabidopsis thaliana</i>

Wang, Yixing; Jha, Ajay Kumar; Chen, Rujin; Doonan, John H.; Yang, Ming

doi:10.1002/dvg.20610

Cited by 22 publications

(20 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The occurrence of tetraploid progeny further suggested that TAM deficiency also affects female meiosis. An identical phenotype has recently been described for several additional tam alleles by other groups (d 'Erfurth et al, 2010;Wang et al, 2010).…”

Section: Smg7 Deficiency Suppresses Premature Meiotic Exit Of Tam Mutsupporting

confidence: 72%

“…In an attempt to rescue the smg7 and tdm1 phenotypes, we sought to inactivate a meiotic cyclin and downregulate meiotic CDK. TAM (cyclin A1;2) appeared to be the obvious candidate, as its function is important for meiotic entry as well as for progression into the second meiosis (Wang et al, 2004b(Wang et al, , 2010d'Erfurth et al, 2010; this study). However, we found that neither the hypomorphic tam-1 nor the null tam-2 alleles alleviate the anaphase II arrest in smg7 mutants or the reentry into the third meiosis in tdm1 plants.…”

Section: Discussionmentioning

confidence: 70%

“…The role of TAM appears to be more complex. TAM is important for entry into the first and second meiotic divisions (Wang et al, 2004b(Wang et al, , 2010d'Erfurth et al, 2010), but its expression seems to be restricted to meiosis I. We suggest that TAM promotes the activities of the core CDK complexes that drive entry into the second meiosis by antagonizing the inhibitory effects of SMG7 and TDM1 ( Figure 8A).…”

Section: Discussionmentioning

confidence: 88%

See 2 more Smart Citations

Meiotic Progression inArabidopsisIs Governed by Complex Regulatory Interactions between SMG7, TDM1, and the Meiosis I–Specific Cyclin TAM

et al. 2010

View full text Add to dashboard Cite

Meiosis is a modified cell division that produces four haploid nuclei from a single diploid cell in two rounds of chromosome segregation. Here, we analyze the role of Arabidopsis thaliana SUPPRESSOR WITH MORPHOGENETIC EFFECTS ON GENITALIA7 (SMG7), THREE DIVISION MUTANT1 (TDM1), and TARDY ASYNCHRONOUS MEIOSIS (TAM) in meiotic progression. SMG7 is a conserved nonsense-mediated mRNA decay factor that is also, in Arabidopsis, essential for completion of meiosis. Examination of activating CYCLIN DEPENDENT KINASE A;1 phosophorylation at Thr-161 suggests that the meiotic arrest observed in smg7 mutants is likely caused by a failure to downregulate cyclin-dependent kinase (CDK) activity at the end of the second meiotic division. Genetic analysis indicates that SMG7 and TDM1 act in the same pathway to facilitate exit from meiosis. We further demonstrate that the cyclin TAM is specifically expressed in meiosis I and has both stimulatory and inhibitory effects on progression to meiosis II. TAM knockouts skip the second meiotic division producing unreduced gametes, but inactivation of SMG7 or TDM1 alleviates TAM's requirement for entry into meiosis II. We propose a model that meiotic progression in Arabidopsis pollen mother cells is driven by a yet to be identified cyclin-CDK activity that is modulated by regulatory interactions between TDM1, SMG7, and TAM.

show abstract

Section: Smg7 Deficiency Suppresses Premature Meiotic Exit Of Tam Mutsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 88%

See 1 more Smart Citation

Meiotic Progression inArabidopsisIs Governed by Complex Regulatory Interactions between SMG7, TDM1, and the Meiosis I–Specific Cyclin TAM

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Two mutants provoke premature exit before meiosis II – osd1 and cyca1;2/tam [15]–[17]. In contrast, tdm mutation prevents exit from meiosis and provokes entry into a third round of division (meiosis III) after meiosis II [13], [20], [21] (Figure 5).…”

Section: Resultsmentioning

confidence: 99%

OSD1 Promotes Meiotic Progression via APC/C Inhibition and Forms a Regulatory Network with TDM and CYCA1;2/TAM

et al. 2012

View full text Add to dashboard Cite

Cell cycle control is modified at meiosis compared to mitosis, because two divisions follow a single DNA replication event. Cyclin-dependent kinases (CDKs) promote progression through both meiosis and mitosis, and a central regulator of their activity is the APC/C (Anaphase Promoting Complex/Cyclosome) that is especially required for exit from mitosis. We have shown previously that OSD1 is involved in entry into both meiosis I and meiosis II in Arabidopsis thaliana; however, the molecular mechanism by which OSD1 controls these transitions has remained unclear. Here we show that OSD1 promotes meiotic progression through APC/C inhibition. Next, we explored the functional relationships between OSD1 and the genes known to control meiotic cell cycle transitions in Arabidopsis. Like osd1, cyca1;2/tam mutation leads to a premature exit from meiosis after the first division, while tdm mutants perform an aberrant third meiotic division after normal meiosis I and II. Remarkably, while tdm is epistatic to tam, osd1 is epistatic to tdm. We further show that the expression of a non-destructible CYCA1;2/TAM provokes, like tdm, the entry into a third meiotic division. Finally, we show that CYCA1;2/TAM forms an active complex with CDKA;1 that can phosphorylate OSD1 in vitro. We thus propose that a functional network composed of OSD1, CYCA1;2/TAM, and TDM controls three key steps of meiotic progression, in which OSD1 is a meiotic APC/C inhibitor.

show abstract

“…Genomic instability refers to the extensive structural changes of the chromosomal DNA that typically take place in the first few generations after polyploidization, such as fusions, fissions, duplications, inversions, translocations and eliminations [77], often coupled to mitotic and meiotic abnormalities [78,79]. Gene expression typically changes markedly [80], in conjunction with widespread epigenetic repatterning [81], in the first few generations after polyploidization.…”

Section: Both Neutral and Adaptive Processes Most Likely Contribute Tmentioning

confidence: 99%

Tangled up in two: a burst of genome duplications at the end of the Cretaceous and the consequences for plant evolution

Vanneste

Maere

Peer

2014

Phil. Trans. R. Soc. B

152

118

View full text Add to dashboard Cite

Genome sequencing has demonstrated that besides frequent small-scale duplications, large-scale duplication events such as whole genome duplications (WGDs) are found on many branches of the evolutionary tree of life. Especially in the plant lineage, there is evidence for recurrent WGDs, and the ancestor of all angiosperms was in fact most likely a polyploid species. The number of WGDs found in sequenced plant genomes allows us to investigate questions about the roles of WGDs that were hitherto impossible to address. An intriguing observation is that many plant WGDs seem associated with periods of increased environmental stress and/or fluctuations, a trend that is evident for both present-day polyploids and palaeopolyploids formed around the Cretaceous–Palaeogene (K–Pg) extinction at 66 Ma. Here, we revisit the WGDs in plants that mark the K–Pg boundary, and discuss some specific examples of biological innovations and/or diversifications that may be linked to these WGDs. We review evidence for the processes that could have contributed to increased polyploid establishment at the K–Pg boundary, and discuss the implications on subsequent plant evolution in the Cenozoic.

show abstract

Polyploidy‐associated genomic instability in Arabidopsis thaliana

Cited by 22 publications

References 32 publications

Meiotic Progression inArabidopsisIs Governed by Complex Regulatory Interactions between SMG7, TDM1, and the Meiosis I–Specific Cyclin TAM

Meiotic Progression inArabidopsisIs Governed by Complex Regulatory Interactions between SMG7, TDM1, and the Meiosis I–Specific Cyclin TAM

OSD1 Promotes Meiotic Progression via APC/C Inhibition and Forms a Regulatory Network with TDM and CYCA1;2/TAM

Tangled up in two: a burst of genome duplications at the end of the Cretaceous and the consequences for plant evolution

Contact Info

Product

Resources

About