Polyprenols from Leaves of Rhus coriaria

Mamatkulova, N. M.; Хидырова, Н. К.; Mamadrakhimov, A.; Shakhidoyatov, Kh. M.

doi:10.1007/s10600-014-1094-6

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…The amounts of PP were determined relative to chromatograms of a standard sample from the ratio of peak areas using the Agilent ChemStation software. The standards were PP from Rhus coriaria leaves [7].…”

Section: Methodsmentioning

confidence: 99%

Polyprenols from Vitis vinifera Leaves and Their Hepatoprotective Activity

et al. 2015

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Polyprenols from leaves of Vitis vinifera L. (Vitaceae) growing in Surkhandarcya Oblast were studied. It was shown that V. vinifera was richer in polyprenols than cultivated varieties. A study of the biological activity of the total polyprenols identified their hepatoprotective activity.The grape Vitis vinifera L. (Vitaceae) is cultivated in the Caucasus, Ukraine, Moldova, Crimea, and Central Asia. The variety V. vinifera is subdivided into three principal ecological-geographical groups. The east-Asian group includes over 40 species of poorly studied grape varieties [1], in the leaves of which sugar, quercetin, inositol, tanning agents, carotene, betaine, tocopherol, ascorbic acid, and organic acids (tartaric, malic, and protocatechoic) and the macroscopic and trace elements K, Ca, Na, Mg, Fe, Al, Zr, Si, P, S, Cl, etc. were detected [2, 3]. The preparation Vivisan for treating varicose veins was developed based on the EtOH extract of cultivated V. vinifera leaves. Tincture of dry grape leaves is used for rinsing and treating several skin diseases. The cold tincture of the leaves is used to strengthen eyes and to treat rheumatism. The decoction of the leaves makes urine basic and gradually dissolves urate kidney stones. Powder of dried grape leaves is inhaled for nose bleeds. The EtOH extract of grape leaves possesses antioxidant activity [4].We studied previously polyprenols (PP) from leaves of the white grape variety Buvaki growing in Tashkent Oblast and developed the optimum extraction method [5]. Herein we present data on the constituent composition of total neutral substances (NS) from leaves of wild red grape and their biological activity.Raw material (200 g) was extracted with EtOH (96%). PP were isolated by condensing the EtOH extract to 1/3 the volume and treating it with weak NaOH solution to remove free acids. The precipitate was saponified by the literature method [6] to afford total NS [10.4 g, 5.2% of air-dried mass (ADM)]. The PP content in total NS was determined by HPLC. The standards were Rhus coriaria PP [7]. Table 1 presents the data.The remaining constituents of total NS were determined by GC/MS analysis without fractionation. According to the HPLC analysis, the PP content in total NS was 45.7% (2.35% of ADM). Other constituents of total NS (GC/MS analysis) included hydrocarbons, phytol, farnesol, geraniol, linalool, ethyl palmitate, etc. (Table 2).The selected conditions did not allow the constituents to be well separated. The main constituent of the total NS was phytol (Table 2), the content of which was 66.15%. Minor constituents were volatile terpenes and hydrocarbons, the contents of which were 2.87%. PP were isolated from the total NS by column chromatography. The yield of the PP fraction was 4.48 g (44.8% of total NS) with PP content 75.2%. IR, PMR, and 13 C NMR spectra were used for the identification. The spectral characteristics of the isolated PP agreed with those in the literature [5,8].PP exhibit various biological activities. The abilities to stimulate regenerative processes in ...

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Section: Methodsmentioning

confidence: 99%

Polyprenols from Vitis vinifera Leaves and Their Hepatoprotective Activity

et al. 2015

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“…Furthermore, hydrocarbon, triterpenoid, and phytol fractions were isolated. These were identified by preparative TLC as before [9]. PP fractions were analyzed using an Agilent 1100 chromatograph with an Eclipse XDB-C-18 column (0.46 u 150 mm) with UV detection at 210 nm.…”

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“…The analysis time was 30 min. The mobile-phase gradient consisted of systems A (MeOH-H 2 O, 9:1, v/v) and B (MeOH-hexane-i-PrOH, 2:1:1) [9]. PP from cotton leaves with a known composition were used as standards [10].…”

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Neutral Constituents of Alcea rosea Leaves

et al. 2015

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Plants of the family Malvaceae are distinguished among leafy plants by high contents of polyprenols in the aerial parts [1][2][3][4]. In continuation of research on polyisoprenoids from plants of this family, we report the polyisoprenoid compositions of two varieties of Alcea rosea L. (rose hollyhock, 1, and ruby hollyhock, 2) growing in Samarkand Province. Plant material was collected on Aug. 26, 2011 during fruiting in Pai Aryk District.Alcea rosea L. is a beautiful garden flower that numbers ~60 varieties distributed in the eastern Mediterranean region, the Middle East, and Central Asia [5]. Flowers of the plant are used in medicine to ameliorate coughing. Various aqueous decoctions and infusions of the plant are used as mucosal, softening, binding, and anti-inflammatory agents [6].Alcohol extracts of the dried and ground plant leaves were obtained by the literature method [7] in yields of 22.5 and 23.2%, respectively. The alcohol extracts were condensed to 1/3 the volume and worked up with weak NaOH solution in order to remove free acids. The residue was saponified as before [8] to isolate total neutral substances (NS) in yields of 8.5% [1, with polyprenol (PP) content 84.7%] and 8.25% (2, with PP content 80.7%) of the air-dried mass (ADM). Then, column chromatography separated the obtained total NS from A. rosea leaves. The adsorbent was KSK silica gel (60-100 mesh); mobile phase, petroleum ether-Et 2 O mixtures (50:1, 30:1, 25:1). Fractions (100 mL) were collected and monitored by TLC. Identical fractions were combined. PP fractions were isolated. The PP yield for 1 was 1.88% (of ADM); for 2, 1.76%. Furthermore, hydrocarbon, triterpenoid, and phytol fractions were isolated. These were identified by preparative TLC as before [9]. PP fractions were analyzed using an Agilent 1100 chromatograph with an Eclipse XDB-C-18 column (0.46 u 150 mm) with UV detection at 210 nm. The mobile phase was a gradient (%B); 0-20 min, 0-75; 20-25 min, 75-100; 25-30 min, 100-0 at flow rate 0.75 mL/min. The analysis time was 30 min. The mobile-phase gradient consisted of systems A (MeOH-H 2 O, 9:1, v/v) and B (MeOH-hexane-i-PrOH, 2:1:1) [9]. PP from cotton leaves with a known composition were used as standards [10]. Table 1 presents the HPLC analytical data. Table 1 shows the constituent ratio of the rose and ruby hollyhock leaf fractions in addition to the PP in each sample. It can be seen that undecaprenol (n = 11) and dodecaprenol (n = 12) dominated the PP-homologs. The decaprenol (n = 10) content in rose hollyhock was 9.4%; in ruby, 8.2%. The tridecaprenol fractions were identical at 8.6%.The other constituents of NS from A. rosea (rose hollyhock) were identified on an Agilent 7890 GC-5975 without additional separation by comparing their retention times with those of known compounds and comparing the mass spectra with hose in the NIST 02MS electronic library. The accuracy of identification was 75-90%. Table 2 presents the results. Table 2 shows that the principal components of the NS were E-sitosterol (20.9%), phytol (20.55%),...

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