Polypyrrole-coated poly(l-lactic acid-co-ε-caprolactone)/silk fibroin nanofibrous membranes promoting neural cell proliferation and differentiation with electrical stimulation

Abstract

Conductive nanofiber membranes were developed by coating Ppy on PLCL/SF nanofibers via in situ oxidative polymerization.

Conductive Scaffolds for Cardiac and Neuronal Tissue Engineering: Governing Factors and Mechanisms

“…The incorporation of conductive polymer elements for neuronal tissue engineering has become quite common. Sun et al prepared an electrospun poly( l ‐lactic acid‐ co ‐e‐caprolactone)/silk fibroin (PLCL/SF) coated with in situ polymerized PPy nanoparticles. Addition of PPy improved scaffold hydrophilicity and conductivity up to 0.1 mS cm −1 .…”

“…These facts demonstrate the necessity of the conductive element in the system to augment neuronal cell development via electrical stimulation. Further emphasizing the promise of conductive polymers for peripheral nerve treatment, Sun et al performed follow up experiments to their 2016 study by using their PLCL/SF/PPy scaffold in vivo . Results of rat sciatic nerve treatment showed matching results with the gold standard autograft in most metrics, save for slightly diminished axonal diameter and myelin thickness, in which the nondoped control paled in comparison.…”

Electroactive Composite of FeCl3‐Doped P3HT/PLGA with Adjustable Electrical Conductivity for Potential Application in Neural Tissue Engineering

“…Nevertheless, the cells displayed better adhesion and spreading on the substrates with higher conductivity (>10 −4 S cm −1 ). To analyze the influence of conductivity on the differentiation of PC12 cells, NGF (100 ng mL −1 ) was introduced into the cell culture system . Under the condition of adding growth factor, some cells are no longer in the state of proliferation after 4 days of culture, and the cells show differentiation behavior and grow synapses.…”

A study on graphene composites for peripheral nerve injury repair under electrical stimulation

“…Sun et al suggested that ES could promote SC proliferation according to the membranes coated with PPY. 24,39,40 However, in other studies, Abigail N. Koppes et al suggested that ES had no signicant inuence on SC proliferation. 8 Therefore, there was discrepancy on the effect of ES on SC proliferation.…”

“…Most importantly, the resistivity of the conductive scaffolds consisting of these polymer materials is in the range of 1000-40 000 U cm. [22][23][24] Therefore, a larger ES intensity may be required, which might damage the neural cells and tissues. An ideal conductive scaffold should provide not only electrical properties but also biocompatibility cues for guiding neural cell growth.…”