Polypyrrole/copper hexacyanoferrate hybrid as redox mediator for glucose biosensors

Abstract

A copper containing Prussian Blue analogue was incorporated into a conducting polypyrrole film. The modified electrode was synthesized through an electrochemical two-step methodology leading to very stable and homogeneous hybrid films. These electrodes were proved to show excellent catalytic properties towards H 2 O 2 detection, with a performance higher than those observed for Prussian Blue and other analogues. Electrochemical impedance spectroscopy experiments demonstrated that the excellent performance of these hybrid films is strongly related to the electronic conductivity of the polymeric matrix that is wiring the copper hexacyanoferrate sites. A glucose biosensor was built-up by the immobilization of glucose oxidase; the sensitivity obtained being higher than other biosensors reported in the literature even in Na + containing electrolytes.

Electrodeposition of a Nanostructured Hybrid Copper Hexacyanoferrate/Polypyrrole Film Using Lyotropic Liquid Crystals and Colloidal Particles Templates. Application to H2O2 Detection.

“…Copper hexacyanoferrate/polypyrrole (CuHCNFe/Ppy) was synthesized in a two step procedure (10). In the first one, the ferricyanide was incorporated in a polypyrrole matrix by cyclic voltammetry.…”

“…Some metal hexacyanoferrates, such as nickel hexacyanoferrate and copper hexacyanoferrate, have already been introduced in a conducting polypyrrole matrix creating hybrid materials of nickel hexacyanoferrate/polypyrrole (NiHCNFe/Ppy) and copper hexacyanoferrate/polypyrrole (CuHCNFe/Ppy) with better mechanical and electrochemical characteristics (9,10).…”

Enzyme-based Electrochemical Biosensors

“…In biosensor construction, PPy is often used as a conducting matrix and thus other organic/inorganic materials could be introduced into the matrix to further improve the performance of the biosensor. For example, stable and homogenous hybrid films consisting of PPy and copper hexacyanoferrate by electrochemical method were synthesized, aiming to obtain an electrocatalyst for H 2 O 2 reduction in the presence of either Na + or K + ions (Fiorito et al, 2006). The constructed biosensor shows excellent catalytic properties towards H 2 O 2 detection, with a performance higher than those observed for Prussian Blue and other analogues due to the electronic conductivity of the polymeric matrix (Fiorito et al, 2006).…”

“…For example, stable and homogenous hybrid films consisting of PPy and copper hexacyanoferrate by electrochemical method were synthesized, aiming to obtain an electrocatalyst for H 2 O 2 reduction in the presence of either Na + or K + ions (Fiorito et al, 2006). The constructed biosensor shows excellent catalytic properties towards H 2 O 2 detection, with a performance higher than those observed for Prussian Blue and other analogues due to the electronic conductivity of the polymeric matrix (Fiorito et al, 2006). In practical, it is important to find ways to obtain PPy polymers with desirable properties for biosensor applications by introducing various dopants.…”

Nanostructured thin films obtained by electrodeposition over a colloidal crystal template: applications in electrochemical devices

“…In this case, the CuHCNFe/ Ppy hybrid compound achieved a sensitivity of 181.10 µA cm -2 mmol -1 L towards H 2 O 2 detection, which is about 1300 times higher than the value achieved without the conducting polymer. 24 The sensitivities presented above are referred to bulk materials and the goal of this work was to produce macroporous CuHCNFe/Ppy mediators with the purpose of making H 2 O 2 detection more efficient. The colloidal particle template method was used to achieve a hybrid inorganic/ organic film with close-packed arrays of interconnected spherical pores, with around 460 nm diameter.…”