Polysaccharide-based hemostats: recent developments, challenges, and future perspectives

Biranje, Santosh; Sun, Jianzhong; Shi, Yifei; Yu, Sujie; Jiao, Haixin; Zhang, Meng; Wang, Qianqian; Jin, Wang; Liu, Jun

doi:10.1007/s10570-021-04132-x

Cited by 22 publications

(14 citation statements)

References 280 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When Cs comes in contact with blood, its cationic amine group interacts with the erythrocyte anionic membrane, assisting Cs in sealing the bleeding site. Controlling hemorrhage with Cs in the form of gauze is not dependent on the coagulation cascade. − It operates by attaching to the tissue surface due to its mucoadhesive feature. Additionally, it has been reported that Cs causes platelet activation and aggregation that would aid in platelet aggregation, while ions such as Ti + , V + , and N would aid in the activation of the coagulation cascade by soluble guanylate cyclase in NO signaling .…”

Section: Resultsmentioning

confidence: 99%

Fabrication of a Chitosan-Based Wound Dressing Patch for Enhanced Antimicrobial, Hemostatic, and Wound Healing Application

Prakash

Venkataprasanna

Vinothkumar

et al. 2023

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Wounds are a serious life threat that occurs in daily life. The complex cascade of synchronized cellular and molecular phases in wound healing is impaired by different means, involving infection, neuropathic complexes, abnormal blood circulation, and cell proliferation at the wound region. Thus, to overcome these problems, a multifunctional wound dressing material is fabricated. In the current research work, we have fabricated a wound dressing polymeric patch, with poly(vinyl alcohol) (PVA) and chitosan (Cs) incorporated with a photocatalytic graphene nanocomposite (GO/TiO2(V-N)) and curcumin by a gel casting method, that focuses on multiple stages of the healing process. The morphology, swelling, degradation, moisture vapor transmission rate (MVTR), porosity, light-induced antibacterial activity, hemolysis, blood clotting, blood abortion, light-induced biocompatibility, migration assay, and drug release were analyzed for the polymeric patches under in vitro conditions. PVA/Cs/GO/TiO2(V-N)/Cur patches have shown enhanced wound healing in in vivo wound healing experiments on Wister rats. They show higher collagen deposition, thicker granulation tissue, and higher fibroblast density than conventional dressing. A histological study shows excellent re-epithelialization ability and dense collagen deposition. In vitro and in vivo analysis confirmed that PVA/Cs/GO/TiO2(V-N) and PVA/Cs/GO/TiO2(V-N)/Cur patches enhance the wound healing process.

show abstract

Section: Resultsmentioning

confidence: 99%

Fabrication of a Chitosan-Based Wound Dressing Patch for Enhanced Antimicrobial, Hemostatic, and Wound Healing Application

Prakash

Venkataprasanna

Vinothkumar

et al. 2023

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

show abstract

“… 30 Therefore, even though many innovative hemostatic agents are being developed, the commercial product must be cost-effective, easily portable, and effectively applied at the injury site under any conditions. 31 …”

Section: Discussionmentioning

confidence: 99%

Safety and Performance of Hemostatic Powders

Szymański¹,

Gołaszewska²,

Małkowska³

et al. 2023

MDER

View full text Add to dashboard Cite

Background Hemorrhage, a sudden and severe leakage of blood due to the disruption of blood vessels, is one of the most common causes of death from injuries worldwide. Severe bleeding accounts for more than 35% of pre-hospital deaths and about 40% of deaths recorded within 24 hours of injury. One of the methods for achieving homeostasis is the use of hemostatic powders. This study compares the basic safety and performance of the most popular hemostatic powders. Methods Basic safety of commercially available products were evaluated using MTT, MEM elution assay, and endotoxin testing. The in vitro performance was evaluated using water absorption capacity, water absorption rate, and adhesion strength assays. Results 4Seal, Starsil, and 4DryField extracts did not cause cytotoxicity in MTT and MEM elution assays. PerClot and SuperClot extracts demonstrated cytotoxic potential in MTT assay, while Arista extract was cytotoxic in both MEM elution and MTT assays. 4Seal has the lowest endotoxin contamination, followed by PerClot, 4DryField, SuperClot, Arista, and Starsil. 4Seal and Starsil showed significantly highest WAR among the tested samples, followed by 4DryField, Arista, PerClot, and SuperClot. Adhesion force is highest for 4Seal, followed by Starsil, PerClot, 4DryField Arista, and SuperClot. Conclusion 4Seal is the most versatile in terms of safety and functional properties compared to 4DryField, Arista, PerClot, Starsil, and SuperClot.

show abstract

“…Because of the moderate reaction condition, TEMPO-mediated oxidation was widely explored to generate the NFC that offers enhanced solubility owing to the high content of the carboxylic acid group on the cellulose surface. The oxidized NFC offers several advantages in terms of its biocompatibility, presence of carboxylic ends allowing easier functionalization, and tunability of the mechanical property, which altogether have gathered much attention for developing next-generation biomaterials. ,,, …”

Section: Introductionmentioning

confidence: 99%

“…The oxidized NFC offers several advantages in terms of its biocompatibility, presence of carboxylic ends allowing easier functionalization, and tunability of the mechanical property, which altogether have gathered much attention for developing next-generation biomaterials. 25,28,42,43 Although the NFC hydrogel has emerged as an efficient biomaterial for various biomedical applications, it lacks biological functionality, which limits the utilization of this classical biopolymer material as an effective scaffold for tissue engineering. However, on the other hand, the supramolecular hydrogels based on bioactive peptides discussed so far have their own limitations in terms of their mechanical rigidity, but their natural design and bioactivity are the beneficial features that can be incorporated in the cellulose-based polymeric gels to enhance their biofunctionality.…”

Section: Introductionmentioning

confidence: 99%

Exploring Supramolecular Interactions between the Extracellular-Matrix-Derived Minimalist Bioactive Peptide and Nanofibrillar Cellulose for the Development of an Advanced Biomolecular Scaffold

Kaur¹,

Sharma²,

Pal³

et al. 2023

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

It has been increasingly evident over the last few years that bioactive peptide hydrogels in conjugation with polymer hydrogels are emerging as a new class of supramolecular materials suitable for various biomedical applications owing to their specificity, tunability, and nontoxicity toward the biological system. Despite their unique biocompatible features, both polymer- and peptide-based scaffolds suffer from certain limitations, which restrict their use toward developing efficient matrices for controlling cellular behavior. The peptide hydrogels usually form soft matrices with low mechanical strength, whereas most of the polymer hydrogels lack biofunctionality. In this direction, combining polymers with peptides to develop a conjugate hydrogel can be explored as an emergent approach to overcome the limitations of the individual components. The polymer will provide high mechanical strength, whereas the biofunctionality of the material can be induced by the bioactive peptide sequence. In this study, we utilized TEMPO-oxidized nanofibrillar cellulose as the polymer counterpart, which was co-assembled with a short N-cadherin mimetic bioactive peptide sequence, Nap-HAVDI, to fabricate an NFC–peptide conjugate hydrogel. Interestingly, the mechanical strength of the peptide hydrogel was found to be significantly improved by combining the peptide with the NFC in the conjugate hydrogel. The addition of the peptide into the NFC also reduced the pore size within NFC matrices, which further helped in improving cellular adhesion, survival, and proliferation. Furthermore, the cells grown on the NFC and NFC–peptide hybrid hydrogel demonstrated normal expression of cytoskeleton proteins, i.e., β-tubulin in C6 cells and actin in L929 cells, respectively. The selective response of neuronal cells toward the specific bioactive peptide was further observed through a protein expression study. Thus, our study demonstrated the collective role of the cellulose–peptide composite material that revealed superior physical properties and biological response of this composite scaffold, which may open up a new platform for biomedical applications.

show abstract

Polysaccharide-based hemostats: recent developments, challenges, and future perspectives

Cited by 22 publications

References 280 publications

Fabrication of a Chitosan-Based Wound Dressing Patch for Enhanced Antimicrobial, Hemostatic, and Wound Healing Application

Fabrication of a Chitosan-Based Wound Dressing Patch for Enhanced Antimicrobial, Hemostatic, and Wound Healing Application

Safety and Performance of Hemostatic Powders

Exploring Supramolecular Interactions between the Extracellular-Matrix-Derived Minimalist Bioactive Peptide and Nanofibrillar Cellulose for the Development of an Advanced Biomolecular Scaffold

Contact Info

Product

Resources

About