Polysaccharide from Inonotus obliquus inhibits migration and invasion in B16-F10 cells by suppressing MMP-2 and MMP-9 via downregulation of NF-κB signaling pathway

Lee, Ki Rim; Lee, Jong Seok; Kim, Young Rae; Song, In Gyu; Hong, Eock Kee

doi:10.3892/or.2014.3103

Cited by 58 publications

(38 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MMPs are partly mediated by the mitogen‐activated protein kinase (MAPK) pathway an MAPK pathway plays a central role in regulating the expression of MMP‐2 and MMP‐9 . MAPK pathway regulated various cellular activities such as proliferation, invasion, metastasis, and death . NK and c‐Jun are the factors of MAPK, here, results indicated that TET decreased p‐JNK and p‐c‐Jun in GBM 8401 cells (Figure C) which means TET suppressed MAPK signal pathway in GBM 8401 cells.…”

Section: Discussionmentioning

confidence: 76%

Tetrandrine inhibits human brain glioblastoma multiforme GBM 8401 cancer cell migration and invasion in vitro

Jiang

Cheng

Kuo

et al. 2018

Environmental Toxicology

View full text Add to dashboard Cite

Tetrandrine (TET) has been reported to induce anti-cancer activity in many human cancer cells and also to inhibit cancer cell migration and invasion. However, there are no reports to show TET inhibits cell migration and invasion in human brain glioblastoma multiforme GBM 8401 cells. In this study, we investigated the anti-metastasis effects of TET on GBM 8401 cells in vitro. Under sub-lethal concentrations (from 1, 5 up to 10 μM), TET significantly inhibited cell mobility, migration and invasion of GBM 8401 cells that were assayed by wound healing and Transwell assays. Gelatin zymography assay showed that TET inhibited MMP-2 activity in GBM 8401 cells. Western blotting results indicated that TET inhibited several key metastasis-related proteins, such as p-EGFR (Tyr1068) , SOS-1, GRB2, Ras, p-AKT (Ser473) and p-AKT (Thr308) , NF-κB-p65, Snail, E-cadherin, N-cadherin, NF-κB, MMP-2 and MMP-9 that were significant reduction at 24 and 48 hours treatment by TET. TET reduced MAPK signaling associated proteins such as p-JNK1/2 and p-c-Jun in GBM 8401 cells. The electrophoretic mobility shift (EMSA) assay was used to investigate NF-κB and DNA binding was reduced by TET in a dose-dependently. Based on these findings, we suggested that TET could be used in anti-metastasis of human brain glioblastoma multiforme GBM 8401 cells in the future. K E Y W O R D S GBM 8401 cells, migration and invasion, MMP-2, NF-κB, Tetrandrine (TET) 1 | INTRODUCTION Malignant glioblastoma multiforme (GBM) are the most common and aggressive malignant primary brain tumor in humans and can be a fatal tumor because of difficulties in treating the related metastasis due to the high proliferation rate and invasiveness. 1-5 Currently, the treatment of GBM is the local irradiation, and conventional chemotherapy with temozolomide (TMZ) or surgical resection in combination with radiotherapy and chemotherapy but the median survival rate is only 8-15 months. 4-6 About 20% of patients after treated with TMZ have shown the clinical toxicity. 7 Due to the side effects of TMZ chemotherapy that leading to the limited efficiency. Thus, bases on the Yun-Lian Lin and Jing-Gung Chung contributed equally to this study.

show abstract

Section: Discussionmentioning

confidence: 76%

Tetrandrine inhibits human brain glioblastoma multiforme GBM 8401 cancer cell migration and invasion in vitro

Jiang

Cheng

Kuo

et al. 2018

Environmental Toxicology

View full text Add to dashboard Cite

show abstract

“…In this study, inhibition of p-p38, p-ERK1/2 and p-JNK1/2 all markedly attenuated MMP-9 expression in 2-CE exposed astrocytes, suggesting that all three kinds of MAPK signal pathways were activated, and collectively involved in modulation of MMP-9 expression in 2-CE exposed astrocytes. Until now, there is a large body of evidence to suggest that MMP-9 expression is regulated by p38, ERKs and JNKs in different cell types (Lee et al, 2014; Kim et al, 2015; Lian et al, 2015). However, as far as we know, this is the first time to discuss the molecular mechanisms of MMP-9 expression regulation in astrocytes exposed to 2-CE.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of Matrix Metalloproteinase-9 in Primary Cultured Rat Astrocytes Induced by 2-Chloroethanol Via MAPK Signal Pathways

Wang

Liao

Sun

et al. 2017

Front. Cell. Neurosci.

View full text Add to dashboard Cite

2-Chloroethanol (2-CE) is one of the reactive metabolites of 1,2-DCE in vivo, which might contribute to brain edema formation induced by 1,2-dichloroethane (1,2-DCE) poisoning. Thus, the purpose of this study was to explore the roles of mitogen-activated protein kinase (MAPK) signal pathways in upregulation of matrix metalloproteinase-9 (MMP-9) in 2-CE exposed rat astrocytes. Expression of p38 MAPK (p38), extracellular signal regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK) and MMP-9 at both protein and gene levels in rat astrocytes were determined using western blot and real-time RT-PCR methods. The results showed that both protein and mRNA levels of MMP-9 in 2-CE exposed astrocytes significantly increased. Meanwhile, protein levels of phosphorylated p38 (p-p38), ERK1/2 (p-ERK1/2) and JNK1/2 (p-JNK1/2) in 2-CE exposed astrocytes also significantly increased. In addition, both protein and mRNA levels of MMP-9 significantly decreased in response to reduced protein levels of p-p38, p-ERK1/2 and p-JNK1/2 achieved by supplement with their specific inhibitors, indicating that activation of MAPK signal pathways might play an important role in upregulation of MMP-9 expression at the transcriptional level in 2-CE exposed astrocytes. Furthermore, since pretreatment of n-acetyl-l-cysteine (NAC), a powerful antioxidant amino acid, could attenuate the elevated levels of MMP-9, p-p38, p-ERK2 and p-JNK1/2 in 2-CE exposed astrocytes, activation of MAPK signal pathways in 2-CE exposed astrocytes could be mediated partially by reactive oxygen species (ROS), which was most likely generated in the metabolism of 2-CE.

show abstract

“…Curcumin was also observed to inhibit the invasion of EC cells at non-cytotoxic concentrations. MMP-2 and -9 play a significant role in the metastasis of tumor cells (14)(15)(16). By degrading the ECM, MMP-2 and -9 make the metastasis of tumor cells possible.…”

Section: Discussionmentioning

confidence: 99%

Curcumin suppresses migration and invasion of human endometrial carcinoma cells

et al. 2015

View full text Add to dashboard Cite

Abstract. Curcumin, a widely used Chinese herbal medicine, has historically been used in anti-cancer therapies. However, the anti-metastatic effect and molecular mechanism of curcumin in endometrial carcinoma (EC) are still poorly understood. The purpose of this study was to detect the anti-metastatic effects of curcumin and the associated mechanism(s) in EC. Based on assays carried out in EC cell lines, it was observed that curcumin inhibited EC cell migration and invasion in vitro. Furthermore, following treatment with curcumin for 24 h, there was a decrease in the expression levels of matrix metalloproteinase (MMP)-2 and -9 as well as proteinase activity in EC cells. Moreover, curcumin treatment significantly decreased the levels of the phosphorylated form of extracellular signalregulated kinase (ERK) 1/2. MEK1 overexpression partially blocked the anti-metastatic effects of curcumin. Combined treatment with ERK inhibitor U0126 and curcumin resulted in a synergistic reduction in MMP-2/-9 expression; the invasive capabilities of HEC-1B cells were also inhibited. In conclusion, curcumin inhibits tumor cell migration and invasion by reducing the expression and activity of MMP-2/9 via the suppression of the ERK signaling pathway, suggesting that curcumin is a potential therapeutic agent for EC.

show abstract

Polysaccharide from Inonotus obliquus inhibits migration and invasion in B16-F10 cells by suppressing MMP-2 and MMP-9 via downregulation of NF-κB signaling pathway

Cited by 58 publications

References 29 publications

Tetrandrine inhibits human brain glioblastoma multiforme GBM 8401 cancer cell migration and invasion in vitro

Tetrandrine inhibits human brain glioblastoma multiforme GBM 8401 cancer cell migration and invasion in vitro

Upregulation of Matrix Metalloproteinase-9 in Primary Cultured Rat Astrocytes Induced by 2-Chloroethanol Via MAPK Signal Pathways

Curcumin suppresses migration and invasion of human endometrial carcinoma cells

Contact Info

Product

Resources

About