Polysaccharide from Plantago asiatica L. attenuates hyperglycemia, hyperlipidemia and affects colon microbiota in type 2 diabetic rats

Arabinoxylan Attenuates Type 2 Diabetes by Improvement of Carbohydrate, Lipid, and Amino Acid Metabolism

“…Venn diagram was also performed to obtain the optimal dose of arabinoxylan in protecting T2D . Moreover, the arabinoxylan possessed high viscosity, high number of branches, and single xylose unit, so it would likely be tolerable and completely fermentable in colon to exert anti‐diabetic effects compared with other polysaccharides . Treatment with arabinoxylan improved host metabolism, such as TCA cycle, lipid, and amino acid metabolism in diabetic rats.…”

“…Seven‐week‐old male inbred Wistar rats (180–200 g) were obtained from Vital River Laboratories (VRL, Beijing, China). All animals were acclimatized under conditions at 22 ± 2 °C and 55 ± 10% relative humidity, 12/12 h light/dark cycle for 1 week before commencement of the animal experiments …”

“…After acclimation, 20 rats were fed a standard normal chow diet and 40 rats were fed a high‐fat diet (HFD). After 8 weeks of dietary manipulation, all the rats were fasted for 12 h, and the rats fed on HFD were injected with streptozotocin (STZ, 30 mg kg –1 body weight [BW]) into the tail vein to induce type 2 diabetes and those fed on basal diet received an equivalent volume of saline . Twenty‐four qualified rats were considered diabetic with the levels of fasting blood glucose higher than 16.7 mmol L –1 .…”

“…Our research group has isolated and purified a novel polysaccharide from the seeds of P. asiatica L. with a molecular weight of 1894 kDa, which was an arabinoxylan characterized by high viscosity and rich in xylose (60%) and arabinose (32%) . The arabinoxylan was found to have notable influence on slowing down glucose diffusion and inhibiting α‐amylase activity in vitro, and hypoglycemic and hypolipidemic effect in vivo . In order to maximize the potentially effective interventions of the arabinoxylan, it is important to fully understand the complicated mechanisms of diabetes on the basis of metabolomics.…”

Investigação do microbioma intestinal em camundongos deficientes em CRAMP submetidos à sepse experimental

“…(27) Condições ambientais, como dieta alimentar, idade e exposição precoce aos microrganismos, também tem sido apontadas como determinantes para a composição do microbioma. (37,38) Sugere-se que a colonização primária prediga a ecologia microbiana e a imunidade tardias. (27)…”