Polysaccharide isolated from Sarcodon aspratus induces RAW264.7 activity via TLR4-mediated NF-κB and MAPK signaling pathways

Wang, Dandan; Pan, Wen-Juan; Mehmood, Shomaila; Cheng, Xiao-Du; Chen, Yan

doi:10.1016/j.ijbiomac.2018.08.147

Cited by 69 publications

(42 citation statements)

References 33 publications

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“…Zhou et al (2017) found that Aps stimulated the key nodes in the TLR4-MyD88-dependent signaling pathway, including TLR4, MyD88, TRAF-6, and NF-κB, both in vitro and in mice. Wang et al (2018) confirmed that the NF-κB mRNA and protein levels were significantly increased in macrophages treated with Aps. These findings were similar to ours, in that both Aps and Gps increased expression of jejunal TLR4, MyD88, and NF-κB proteins, which means that the plant polysaccharides promoted the expression of proteins involved in the TLR4 signaling pathway.…”

Section: Discussionsupporting

confidence: 58%

Astragalus and Ginseng Polysaccharides Improve Developmental, Intestinal Morphological, and Immune Functional Characters of Weaned Piglets

et al. 2019

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Antibiotic resistance is a major issue in animal industries and antibiotic-free alternatives are needed to treat infectious diseases and improve performance of pigs. Plant extracts have been suggested as a potential solution. The present study was conducted to investigate the effects of Astragalus polysaccharides (Aps) and ginseng polysaccharide (Gps) on growth performance, intestinal morphology, immune function, volatile fatty acids (VFAs), and microfloral community in weaned piglets. A total of 180 weaned piglets were randomly divided into three treatment groups during a 28-days feeding experiment, including a basal diet (Con), basal diet supplemented with 800 mg/kg Aps (Aps), and basal diet supplemented with 800 mg/kg Gps (Gps). Results showed that both Aps and Gps increased body weight, average daily gain and feed conversion rate, and reduced the rate of diarrhea. Gps also decreased aspartate aminotransferase compared to the Con piglets after 14 days. No significant effects on alanine aminotransferase were observed. Both Aps and Gps piglets exhibited higher serum immunoglobulin M levels after 14 and 28 days, and also decreased jejunal crypt depth, increased jejunal villus length and villus height/crypt depth ratio, and increased expression of toll-like receptor 4, myeloid differentiation primary response 88, nuclear factor-kappa B proteins in the jejunum. Aps and Gps piglets also had higher concentrations of acetic acid, isobutyric acid, and butyrate in their colon. Data of high-throughput sequencing revealed that Aps and Gps affected bacterial quantity and diversity in the colon. Species richness and evenness were higher in both Aps and Gps piglets than the control piglets. Aps and Gps piglets also had a higher relative abundance of Lachnospiraceae and Anaerostipes , and the Aps piglets had a higher relative abundance of Lactobacillus gasseri and L. amylovorus . Therefore, dietary supplementation with Aps and Gps could be beneficial for optimizing the performance of industry pigs and reducing dependence on antibiotics. Furthermore, Plant polysaccharides play a great role in promoting the sustainable development of animal husbandry.

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Section: Discussionsupporting

confidence: 58%

Astragalus and Ginseng Polysaccharides Improve Developmental, Intestinal Morphological, and Immune Functional Characters of Weaned Piglets

et al. 2019

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“…TLR4 is critical in immune responses and involved mainly in inflammation responses (90). TLR4 can be recognized and activated by many stimuli such as polysaccharides, and its expression increases, and then activates PI3K/Akt and MAPKs pathways, with introduction of a pool of inflammatory mediators (30, 34, 43, 87, 91). In addition, NF-κB involves in macrophage activation as well (24, 34, 92, 93).…”

Section: Discussionmentioning

confidence: 99%

“…A preliminary yeast two-hybrid experiment showed that rFIP-glu did not interact with an extracellular part of TLR2 (Data not shown), suggesting TLR2 possibly was not a receptor of rFIP-glu as well. It is noteworthy that some active substances can activate all of these pathways (34, 57, 88), while some can only activate one or more (30, 87, 94). We next used specific PI3K/Akt and MAPKs pathway inhibitors to clarify whether these signaling pathways were involved in macrophage activation induced by rFIP-glu.…”

Section: Discussionmentioning

confidence: 99%

“…Macrophages can be activated to an inflammation-promoting phenotype through members of the Toll-like receptor (TLR) family such as TLR4 (20, 27, 28). Activated TLRs induce activation of specific intracellular pathways including phosphoinositide 3 kinases (PI3K/Akt), mitogen activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB) (21, 29, 30). PI3K/Akt signaling pathway participates in macrophage polarization (31–34), while MAPKs, including extracellular signal-related kinase (ERK)-1/2, p38 and c-Jun NH2-terminal kinase (JNK), and NF-κB are classic inflammation related signals and induce the expression of pro-inflammatory mediators (35–38).…”

mentioning

confidence: 99%

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Immunomodulatory activity of Ganoderma lucidum immunomodulatory protein via PI3K/Akt and MAPK signaling pathways in macrophage RAW264.7 cells

Chang

et al. 2018

Preprint

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Ganoderma lucidum, a traditional edible and medicinal fungus, holds an important status in health care systems in China and other Asian countries. Fungal immunomodulatory protein (FIP), one of the active ingredients isolated from G. lucidum, is a class of naturally occurring proteins and possesses potential biological functions. This study was conducted to explore the molecular mechanism of its immunomodulatory potency in immune responses of macrophages. In vitro assays of biological activity indicated that rFIP-glu significantly activated macrophage RAW264.7 cells, and possessed the ability of pro- and anti-inflammation the cells. RNA sequencing analysis showed that macrophage activation involved Toll-like receptors and mitogen-activated protein kinases pathways. Furthermore, qRT-PCR indicated that phosphoinositide 3 kinase inhibitor LY294002 blocked the mRNA levels of MCP-1, MEK1/2 inhibitor U0126 reduced the mRNA levels of TNF-α and MCP-1, and JNK inhibitor SP600125 prevented the up-regulation of iNOS mRNA in the rFIP-glu-induced cells. FIP-glu mediated these inflammatory effects not through a general pathway, instead through a different pathway for different inflammatory mediator. These data indicate the possibility that rFIP-glu has an important immune-regulation function and thus has potential therapeutic uses.

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“…The study about the polysaccharide from Sarcodon aspratus , for instance, showed that its immunostimulatory activity not only enhanced the phagocytic function of RAW264.7 cells, but also increased the production of nitric oxide (NO), reactive oxygen species (ROS), and cytokines (TNF-α and IL-6) [ 13 ]. Similar results were also found in a low-molecular-weight β-glucan from Durvillaea Antarctica , which could significantly activate RAW264.7 cells to release NO, ROS, TNF-α, MCP-1 and IL-1β, and exerted stronger immunostimulatory activity on RAW264.7 cells compared with lentinan, in many aspects [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Physicochemical Characteristics and Macrophage Immunostimulatory Activities of Polysaccharides from Chlamys farreri

Shi

Liu

et al. 2020

Marine Drugs

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To address the structure–activity relationship of Chlamys farreri polysaccharides on their immunostimulatory efficacy, two polysaccharides (CFP-1 and CFP-2) were extracted from Chlamys farreri by hot water extraction, and separated through column chromatography. The isolated CFPs were chemically analyzed to clarify their physicochemical characteristics and cultured with murine macrophage RAW264.7 cells, in order to evaluate their immunostimulatory efficacy. Despite the fact that both CFP-1 and CFP-2 were mainly comprised of glucose lacking the triple-helix structure, as revealed through preliminary physicochemical analyses, obvious differences in regard to molecular weight (Mw), glucuronic acid content (GAc) and branching degree (BD) were observed between CFP-1 and CFP-2. In in vitro immunostimulatory assays for macrophage RAW264.7 cells, it was demonstrated that CFP-2 with larger Mw, more GAc and BD could evidently promote phagocytosis and increase the production of NO, IL-6, TNF-α and IL-1β secretion, by activating the expression of iNOS, IL-6, TNF-α and IL-1β genes, respectively. Hence, CFP-2 shows great promise as a potential immunostimulatory agent in the functional foods and nutraceutical industry, while CFP-1, with lower molecular weight, less GAc and BD, displays its weaker immunostimulatory efficacy, based on the indistinctive immunostimulatory parameters of CFP-1.

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Polysaccharide isolated from Sarcodon aspratus induces RAW264.7 activity via TLR4-mediated NF-κB and MAPK signaling pathways

Cited by 69 publications

References 33 publications

Astragalus and Ginseng Polysaccharides Improve Developmental, Intestinal Morphological, and Immune Functional Characters of Weaned Piglets

Astragalus and Ginseng Polysaccharides Improve Developmental, Intestinal Morphological, and Immune Functional Characters of Weaned Piglets

Immunomodulatory activity of Ganoderma lucidum immunomodulatory protein via PI3K/Akt and MAPK signaling pathways in macrophage RAW264.7 cells

Comparison of Physicochemical Characteristics and Macrophage Immunostimulatory Activities of Polysaccharides from Chlamys farreri

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