Polysaccharide of Alocasia cucullata Exerts Antitumor Effect by Regulating Bcl-2, Caspase-3 and ERK1/2 Expressions during Long-Time Administration

Zhou, Qichun; Xiao, Shengnan; Lin, Ru-kun; Li, Chan; Chen, Zhijie; Chen, Yifei; Luo, Chaohua; Mo, Zhixian; Lin, Yingbo

doi:10.1007/s11655-023-3700-6

Cited by 3 publications

(1 citation statement)

References 26 publications

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“…Cleaved-Caspase3 is an active form of Caspase3, and Bcl-2 is an anti-apoptosis protein in Bcl-2 family. Overexpression of Bcl-2 may form a heterodimer with Bax, thereby playing an anti-apoptosis role (Zhou et al, 2023). The results of this study revealed that GA interference considerably downregulated the protein levels of Caspase3 and Bcl-2, elevated the cleaved-Caspase3 level, and raised the ratio of cleaved-Caspase3/ Caspase3 in cells.…”

Section: Discussionmentioning

confidence: 72%

Glycyrrhizic acid inhibits DNA damage repair and enhances cisplatin‐induced apoptosis of melanoma cells

Bian,

Niu,

et al. 2024

Chem Biol Drug Des

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This research was designed to prospect the mechanism and impact of glycyrrhizic acid (GA) on DNA damage repair and cisplatin (CP)‐induced apoptosis of melanoma cells. First, human melanoma cell SK‐MEL‐28 was stimulated using GA for 24, 48, and 72 h. Then, the optimal treatment time and dosage were selected. After that, cell counting kit‐8 (CCK‐8) was employed for testing the cell viability, flow cytometry for the apoptosis, comet assay for the DNA damage of cells, and western blot for the cleaved‐Caspase3, Caspase3, Bcl‐2, and γH2AX protein expression levels. The experimental outcomes exhibited that as the GA concentration climbed up, the SK‐MEL‐28 cell viability dropped largely, while the apoptosis level raised significantly, especially at the concentration of 100 μm. In addition, compared with GA or CPtreatment only, CP combined with GA notably suppressed the viability of melanoma cells and promoted cell apoptosis at the cytological level. At the protein level, the combined treatment notably downregulated the Bcl‐2 and Caspase3 expression levels, while significantly upregulated the cleaved‐Caspase3 and γH2AX expression levels. Besides, CP + GA treatment promoted DNA damage at the DNA molecular level. Collectively, both GA and CP can inhibit DNA damage repair and enhance the apoptosis of SK‐MEL‐28 cells, and the synergistic treatment of both exhibits better efficacy.

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Section: Discussionmentioning

confidence: 72%