Polysaccharides derived from Shenling Baizhu San improve colitis via modulating tryptophan metabolism in mice

Lv, Weijie; Ma, Yi-Mu; Huang, Jieyi; He, Shiqi; Li, Shu-peng; Lin, Jenshan; Chen, Rong; Lun, Jian-chi; Liu, Ju; Guo, Shining

doi:10.1016/j.ijbiomac.2022.09.246

Cited by 33 publications

(21 citation statements)

References 59 publications

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“…35 In addition, increased tryptophan metabolism is associated with the alleviation of DSS-inflicted colitis. 36 Our result showed that the levels of 4-(2-aminophenyl)-2,4-dioxobutanoic acid, kynurenic acid, quinolinic acid, 5-hydroxyindoleacetate, and 5-hydroxyindoleacetic acid of the ABP-2 group were 1.17-, 1.23-, 1.38-, 1.16-, and 1.24-fold higher than those of the Model group. In general, the metabolic derivatives of tryptophan metabolism might be potential aryl hydrocarbon receptor (AHR) ligands to impact colitis by regulating immune response.…”

Section: Resultsmentioning

confidence: 49%

Agaricus bisporus polysaccharides ameliorate ulcerative colitis in mice by modulating gut microbiota and its metabolism

Liu,

Wang,

Yuan

et al. 2024

Food Funct.

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Section: Resultsmentioning

confidence: 49%

Agaricus bisporus polysaccharides ameliorate ulcerative colitis in mice by modulating gut microbiota and its metabolism

Liu,

Wang,

Yuan

et al. 2024

Food Funct.

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“…To further investigate the biochemical mechanisms involved in improving UC symptoms with NEPs, we evaluated whether NEP intake increased the expression of tight-junction (TJ) proteins by immunohistochemical image analysis in colitis mice. , As shown in Figure A,B, the expression levels of colonic ZO-1 and occludin as TJ proteins in DSS-fed mice were markedly reduced relative to NC mice. Strikingly, supplementation of NEPs could increase expression of these proteins, different from EPs and DFs ( p < 0.05, p < 0.01), indicating that NEPs could improve intestinal barrier damage.…”

Section: Resultsmentioning

confidence: 99%

Colon-Targeted Release of Turmeric Nonextractable Polyphenols and Their Anticolitis Potential via Gut Microbiota-Dependent Alleviation on Intestinal Barrier Dysfunction in Mice

Yang

et al. 2023

J. Agric. Food Chem.

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Solid evidence has emerged supporting the role of nonextractable polyphenols (NEPs) and dietary fibers (DFs) as gut microbiota modulators. This study aims to elucidate gut microbiota-dependent release of turmeric NEPs and examine the possible anti-inflammatory mechanism in the dextran sulfate sodium-induced ulcerative colitis (UC) model. 1.5% DSS drinking water-induced C57BL/6J mice were fed a standard rodent chow supplemented with or without 8% extractable polyphenols (EPs), NEPs, or DFs for 37 days. The bound curcumin, demethoxycurcumin, and bisdemethoxycurcumin in NEPs were released up to 181.5 ± 10.6, 65.2 ± 6.0, and 69.5 ± 7.6 μg/mL by in vitro gut microbiota-simulated fermentation and released into the colon of NEP-supplemented mice by 5.7-, 11.0-, and 7.8-fold higher than pseudo germ-free mice, respectively (p < 0.05). NEPs also enhanced the colonic microbiota-dependent production of short-chain fatty acids in vitro and in vivo (p < 0.05). Interestingly, NEP feeding significantly improved the DSS-caused gut microbiota disorder, epithelial barrier damage, and inflammation of UC mice better than EPs or DFs (p < 0.05). Meanwhile, the pseudo germ-free mice supplemented with NEPs failed to ameliorate UC symptoms. These findings manifest that turmeric NEPs as macromolecular carriers exert the target delivery of polyphenols into the colon for regulating gut microbiota to restore the impaired gut barrier function for alleviation of inflammation.

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“…As IL-10 is reported to be produced by Tregs [ 12 ], the reason for the increase in IL-10 in the DSS + Treg group may have been the induction of Tregs by Trp. It has also been reported that compounds that modulate the metabolism of Trp by the gut microbiota induced IL-10 and suppressed inflammation in a mouse colitis model [ 35 ]. In addition, TNF-α is one of the most important cytokines involved in inflammation in IBD, and TNF-α inhibitors such as infliximab [ 36 ] and adalimumab [ 37 ] have shown high remission rates in both UC and CD.…”

Section: Discussionmentioning

confidence: 99%

Immunoregulatory Effects of Elemental Diet and Its Ingredient, Tryptophan, via Activation of the Aryl Hydrocarbon Receptor in Mice

Kubota,

Imai,

Aoyagi

et al. 2024

IJMS

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Inflammatory bowel disease (IBD) is characterized by chronic intestinal inflammation and its treatment varies widely; however, when inflammation is high, a complete nutrient containing pre-digested elemental diet (ED) is used to preserve the intestinal tract. In this study, we investigated the mechanisms underlying the effectiveness of EDs for IBD using mice. C57BL/6 mice were orally treated with the ED (5 mL/day) and its ingredient L-tryptophan (Trp) (1–100 mg/kg), respectively. Flow cytometry analysis revealed that treatment with the ED and Trp (10 and 100 mg/kg) significantly increased the percentage of splenic CD4+-/CD25+-/Foxp3+ regulatory T cells (Tregs). In the 2% DSS-induced colitis-mouse model, Trp administration (100 mg/kg) led to a significant decrease in TNF-α and increase in IL-10 in the serum as well as a significant decrease in the inflammation score. Furthermore, the aryl hydrocarbon receptor (AhR) agonistic activity, which is a key function of Treg induction, of Trp and 15 Trp metabolites was characterized using a highly sensitive DR-EcoScreen cell assay. Five Trp metabolites, including L-kynurenine, acted as AhR agonists, while Trp did not. Taken together, these results suggest that the ED treatment has a Trp-dependent immunoregulatory effect, and several Trp metabolites that activate the AhR might contribute to induction of remission in patients with IBD.

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Polysaccharides derived from Shenling Baizhu San improve colitis via modulating tryptophan metabolism in mice

Cited by 33 publications

References 59 publications

Agaricus bisporus polysaccharides ameliorate ulcerative colitis in mice by modulating gut microbiota and its metabolism

Agaricus bisporus polysaccharides ameliorate ulcerative colitis in mice by modulating gut microbiota and its metabolism

Colon-Targeted Release of Turmeric Nonextractable Polyphenols and Their Anticolitis Potential via Gut Microbiota-Dependent Alleviation on Intestinal Barrier Dysfunction in Mice

Immunoregulatory Effects of Elemental Diet and Its Ingredient, Tryptophan, via Activation of the Aryl Hydrocarbon Receptor in Mice

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